The present application provides a method for preparing mesenchymal stem cell-derived exosomes, the method including: obtaining a cell sample from mesenchymal stem cells and sub-culturing the cell sample, culturing the sub-cultured cells in a substrate medium containing a protein synthesis inhibitory enzyme, and then obtaining a cell culture solution, and isolating exosomes from the cell culture solution, and a cell culture solution produced therefrom. The method for preparing exosomes of the present application has an advantage in that high purity and high concentration exosomes can be isolated.

The present disclosure relates to a composition containing extracellular vesicles isolated from skeletal muscle-derived stem cells as active ingredients, which exhibits the effect of skin aging delay, wound healing, scar reduction and skin whitening, thereby improving skin condition. The present disclosure can not only utilize tissues wasted after surgical operation, thus allowing easy supply of raw materials, but also takes advantage of natural substances, thereby being free from the risk of side effects even upon long-term administration. The composition of the present disclosure promotes the secretion of collagen, reduces reactive oxygen species and promotes re-epithelialization at wound site while remarkably inhibiting melanocyte proliferation, tyrosinase activity and melanogenesis. Accordingly, it can facilitate effective recovery from skin tissue damage caused by oxidative stress, physical wound, intrinsic aging at cell or tissue levels, or hyperpigmentation.

A use of acellular adipose tissue extract in promoting hair growth and retention. The acellular adipose tissue extract contains one or more components selected from the following: an insulin-like growth factor, a brain-derived neurotrophic factor, a glia-derived neurotrophic factor, a vascular endothelial growth factor, a liver growth factor, transforming growth factor β, a basic fibroblast growth factor, a platelet-derived growth factor, an epidermal growth factor, a granulocyte colony stimulating factor, or a combination thereof.

An exosome-based skincare product containing exosomes excreted by lab cultured human umbilical mesenchymal stem cells within a solvent. Optional ingredients such as skin-conditioning agents, antioxidants, surfactants, buffering agents, viscosity decreasing agents, viscosity increasing agents, peptides, binders, and/or humectants may be included.

The present invention relates to a composition for hair regeneration comprising a macrophage-derived extracellular vesicle mimetics, and since macrophage-derived extracellular vesicle mimetics are well absorbed into dermal papilla cells, have an excellent effect in promoting the proliferation of dermal papilla cells, as well as having an activity to increase the level of hair-induced proteins, it is excellent in the effects of proliferation of dermal papilla cells and hair increase and thus a composition comprising macrophage-derived extracellular vesicle mimetics can be provided for hair regeneration.

The present disclosure methods, compositions and kits for the manufacture of extracellular matrix (ECM), wherein the methods compositions and kits comprise aspartyl alanyl diketopiperazine (DA-DKP).

Provided are a composition for ciliogenesis, containing a mesenchymal stem cell or a culture medium of mesenchymal stem cell. The mesenchymal stem cell or the culture medium of mesenchymal stem cell increases the number and promotes growth of primary cilia in cells. The mesenchymal stem cell or the culture medium of mesenchymal stem cell can be used as a pharmaceutical composition for preventing or treating diseases caused by ciliopathy or ciliary impairment. The mesenchymal stem cell or the culture medium of mesenchymal stem cell can be used as a cosmetic composition. A method for preventing or treating ciliopathy or ciliary impairment is disclosed.

Cosmetic formulations and compositions customized and/or personalized for rejuvenation of skin and/or hair are described. The cosmetic formulations are customized and/or personalized to include an extract and/or one or more molecular components in amounts and ratios naturally produced by rejuvenated cells or by cells modified toward rejuvenation. These cells are of skin and/or hair origin that have been reprogrammed, in which the skin and/or hair cells, having been rejuvenated and/or modified from the original cell, are initially obtained from a subject for which a customized cosmetic composition is for, or from a subject representative of a person for which a customized cosmetic composition is for. Reprogramming of the skin and/or hair cells provide rejuvenated cells, embryonic stem cell-like pluripotent cells, and/or transdifferentiated or differentiated young or younger cells that have been transdifferentiated from the rejuvenated or modified cells or differentiated from the embryonic stem cell-like pluripotent cells. Methods of selecting, preparing, and formulating the customized cosmetic products are also described.

A method, and formulations for the method, for the treatment of hair loss is herein described. Typically, the method involves applying an exosome-based skincare product to skin affected by hair loss, such as the skin of the scalp. The exosome-based skincare product contains exosomes produced by and excreted from human umbilical mesenchymal stem cells cultured in vitro under specific conditions and then contained within a solvent. The hair loss may be caused by androgenic alopecia, alopecia areta, or telogen effulvium.

The present invention relates to a composition for stimulating hair growth or preventing hair loss, which contains a conditioned medium or extract of neural stem cells (NSCs) isolated from the ventricular zone of the human brain, and to a preparation method thereof. The conditioned medium or extract of neural stem cells according to the present invention contains various growth factors and cytokines, and thus has an excellent effect on the stimulation of hair growth. Thus, it is useful for hair growth stimulation and hair loss prevention.