The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.

The present invention relates to: a method for preparing a stem cell culture solution which contains growth factors and cytokines and is obtained by culturing, in a serum-free medium, mesenchymal stem cells cultured in a medium containing human platelet lysates (hPLs); and a cosmetic composition using the stem cell culture solution. When the method for preparing a mesenchymal stem cell culture solution obtained from mesenchymal stem cells cultured in an hPL-containing medium is used, it is possible to obtain a mesenchymal stem cell culture solution which has a higher amount of cytokines and growth factors than that of a mesenchymal stem cell culture solution obtained from mesenchymal stem cells cultured in an FBS-containing medium, and has excellent efficacy in the promotion of human dermal fibroblast proliferation, collagen synthesis, elastin synthesis, inhibition of melanin synthesis, and hair growth. The mesenchymal stem cell culture solution can be used to produce cosmetics having effects of skin regeneration, wrinkle improvement, skin elasticity enhancement, skin whitening, and hair growth, and thus can be widely used in the cosmetic field and the pharmaceutical industry.

The disclosure provides a salivary gland (SG) cell sheet comprising one or more layers of confluent SG cells (e.g. submandibular gland (SMG) cells). Methods of treating a wound in a salivary gland (SG), treating hyposalivation, and treating irradiation damage in an SG are also provided. The disclosure also provides a method for producing SG cell sheets comprising culturing SG cells in culture solution on a temperature-responsive polymer which has been coated onto a substrate surface of a cell culture support, wherein the temperature-responsive polymer has a lower critical solution temperature in water of 0-80° C.; adjusting the temperature of el the culture solution to below the lower critical solution temperature, whereby the substrate surface is made hydrophilic and adhesion of the cell sheet to the surface is weakened; and detaching the cell sheet from the culture support.

A skin care moisturizing, exfoliating and light reflecting oil includes a plant extract base, for example a composition of coconut oil and hibiscus oil which accounts for less than half of the total volume of the skin care oil. The moisturizing skin care oil also includes one or more essential oils disposed in the plant extract base which essential oils contain essential fatty acids while providing at least one skin softening emollient. Importantly, the moisturizing skin care oil includes an exfoliating and light reflecting powder dispersed within the essential oils. Finally, the moisturizing skin care oil includes a stabilizer, such as vitamin E.

A composition with exogenous mitochondria as active ingredients, and a use thereof and a cell repairing method therefor. The composition includes exogenous mitochondria and at least one pharmaceutically or cosmetically acceptable carrier. The composition may further include an adjuvant, and the adjuvant is selected from a group consisting of serum, plasma, complement and at least the above two ingredients. The exogenous mitochondria are obtained from cells by a centrifugal purification method.

A method of culturing an allogeneic immune cell according to an embodiment of the present disclosure efficiently amplifies and activates natural killer cells (NK cells), which are effective in treating malignant tumors, by culturing lymphocytes derived from the blood of healthy donors rather than patients to whom an immune cell therapeutic agent is to be administered.

The present invention relates to a method for differentiating from human adipose-derived mesenchymal stem cells to dermal papilla cells using a differentiation induction medium composition in a cell culture plate for inducing differentiation, including gelatin, and to the medium composition. The plate comprising the gelatin of the present invention can exhibit the effect of inducing direct cross-differentiation from human adipose-derived mesenchymal stem cells to dermal papilla cells, and the effect of efficiently enabling mass cultivation ex vivo at low cost by using an economical material. In addition, the dermal papilla cells differentiated from human adipose-derived mesenchymal stem cells of the present invention can be used as a cell therapy composition for preventing or treating hair loss.

The present invention relates to the fields of medicine, cell biology, molecular biology and genetics. In particular, the present invention provides methods to isolate and purify microvesicles from cell culture supernatants and biological fluids. The present invention also provides pharmaceutical compositions of microvesicles to promote or enhance wound healing, stimulate tissue regeneration, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth and/or mobility, or alter normal cell growth and/or mobility. The present invention also provides compositions of microvesicles to be used as diagnostic reagents, and methods to prepare the compositions of microvesicles.

A composition for alleviating facial redness containing stem cell-derived exosomes as an active ingredient and a method of reducing facial redness are provided. When a facial skin is treated with the composition for alleviating facial redness containing stem cell-derived exosomes as an active ingredient, the composition exhibits an excellent effect on skin care by reducing the redness of the face and improving the appearance of the face.

A method for producing exosomes is provided. The method includes culturing animal cells in a medium containing TNF-α and interferon-γ. This method is possible not only to increase production of exosomes to be isolated from a cell-derived conditioned medium, but also to exhibit an excellent effect of enhancing bioactivity of exosomes, such as increasing elastin synthesis of exosomes.