Methods for stimulating in-vitro Paecilomyces hepiali or Hirsutella sinensis growth, and nutritional supplement formulations containing such fungal strains are disclosed and described. In some embodiments, such a method may include providing a an effective amount of a P. hepiali fungus strain for use as a stimulating agent on a culture medium for which H. sinensis is grown and regulating the temperature to induce and maintain the growth of the H. sinensis fungus. Alternatively, a P. hepiali fungus may be grown in a similar manner using H. sinensis as the stimulating agent. In some aspects, the growth of one fungus may be sequentially switched with the other in order to improve fungi potency.

The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.