The present invention aims to provide a meniscus regeneration material having high meniscus regeneration ability. The present invention provides a meniscus regeneration material including a protein (A), wherein the protein (A) contains at least one of a polypeptide chain (Y) and a polypeptide chain (Y′); a total number of the polypeptide chain (Y) and the polypeptide chain (Y′) in the protein (A) is 1 to 100; the polypeptide chain (Y) includes 2 to 200 tandem repeats of at least one amino acid sequence (X) selected from the group consisting of an amino acid sequence VPGVG (1) set forth in SEQ ID No: 1, an amino acid sequence GVGVP (4) set forth in SEQ ID No: 4, an amino acid sequence GPP, an amino acid sequence GAP, and an amino acid sequence GAHGPAGPK (3) set forth in SEQ ID No: 3; the polypeptide chain (Y′) includes the polypeptide chain (Y) in which 5% or less of amino acid residues are replaced by at least one of a lysine residue and an arginine residue, and a total number of the lysine residue and the arginine residue is 1 to 100; the protein (A) has a total percentage of β-turns and random coils of 60 to 85% as determined by circular dichroism spectroscopy; and when the amino acid sequence (X) in which 60% or less of amino acid residues are replaced by at least one of a lysine residue and an arginine residue is denoted as an amino acid sequence (X′), a ratio of a total number of amino acid residues in the amino acid sequence (X) and the amino acid sequence (X′) in the protein (A) to the number of all amino acid residues in the protein (A) is 50 to 70%.

The present invention relates to a method of manufacturing an animal cartilage-derived injectable composition, an injectable composition manufactured by the method, and a use thereof.

The injectable composition of the present invention includes a collagen-containing biomaterial in a formulation injectable into joint cavity and may induce cartilage tissue regeneration by repairing tissue via direct injection to a target region without surgical incision. In addition, the injectable composition may be used as a therapeutic agent for arthritis by alleviating osteoarthritis not only because the animal cartilage-derived extracellular matrix contained in the injectable composition protects articular cartilage tissue but also because an environment capable of regenerating damaged articular tissue is created by inducing differentiation of intra-articular stem cells into chondrocytes.

An object of the invention is to provide a cartilage regenerative material that suppresses infiltration of fibrous soft tissue and brings about satisfactory cartilage regeneration, and a method for producing the cartilage regenerative material. Provided is a cartilage regenerative material including a porous body of a biocompatible polymer and a biocompatible polymer film, in which the porous body contains chondrocytes and cartilage matrix, and the cartilage matrix exists in a region of 10% or more of a region extending from the surface of the transplant face of the porous body to a depth of 150 μm along the thickness.

The method of treating osteoarthritis of the knee includes replacing the entire cartilage of the femoral condyle, proximal tibia, and two menisci using allograft, while retaining the native cruciate ligaments. The method includes resection of the proximal tibia and the femoral condyle. A first allograft is configured to replace the femoral condyle and includes whole cartilage with about 5 mm to about 7 mm of cancellous bone attached. The first allograft can have a slot or opening through which native cruciate ligaments may pass. A second allograft can replace the upper end of the tibia and menisci. The second allograft can include a slot or opening through which native cruciate ligaments may pass. The first and second allografts can be fixed using cancellous screws. The procedure can provide patients with full knee flexion, and thereby enable kneeling, e.g., as required in the Islamic prayers, full kneeling, or any other activity which requires full flexion of the knee and kneeling.

A pre-sutured allograft construct and method of manufacture for repairing, replacing, reconstructing, or augmenting a hip or shoulder labrum may include a folded tissue portion extending from a first end to a second end and forming top, middle, and bottom folds. A stitched pattern secures the folded tissue portion into a graft roll having an overall length extending from a first adjustable region, through a central region, and through a second adjustable region. A continuous series of whip stitches extends from the first adjustable region, through the central region, and through the second adjustable region. A series of triple circumferential stitches overlays the whip stitches in the first and the second adjustable regions, while a series of circumferential stitches alternates with the whip stitches in the central region. The construct is pre-manufactured as an allograft product, but is adjustable during the surgical procedure within the body. Other embodiments are also disclosed.

The present invention relates to a method of manufacturing collagen for restoring cartilage tissue usable for a joint in an injection manner, and a usage method thereof. The present invention provides a method of obtaining a high-concentration collagen-solution injection by aseptically filling an injection container with collagen separated from pig skin tissue, and a method of using the high-viscosity collagen loaded into the injection container for the purpose of cartilage restoration. Cartilage tissue regeneration is effectively induced when tissue restoration is implemented with respect to a cartilage-deficient portion using collagen, which is a biocompatible material, in a form that is capable of being injected into an application site with an injection needle without a surgical incision. Accordingly, restoration and regeneration of cartilage are easily and quickly induced in an animal, excluding a human, while relieving the burden related to surgery.

Provided herein are meniscus implant compositions, as well as method for making and using the same. The subject meniscus implants find use in repairing and/or replacing damaged or diseased meniscal tissue in a mammalian subject.

The present invention relates to a monolithic multi-layered material having at least a first layer, from which anisotropic pores originate, and a second layer, in which the anisotropic pores continue. The present invention further relates to a monolithic medical material having at least a first layer, from which anisotropic pores originate, and a second layer, in which the anisotropic pores continue. The present invention further relates to a process for the production of a multi-layered material having anisotropic pores. It further relates to a multi-layered material which can be produced by the process according to the invention.

Disclosed is a method for differentiating induced pluripotent stem cells (iPSCs) into chondrocytes and integrating them into a matrix/scaffold to provide a cartilage implant. The method comprises the steps of seeding a surface of a substrate with iPSCs. The surface is coated with nanoparticles in a particle density of at least 500 particles/μm2, and parts of the surface in between said nanoparticles are coated with a coating agent. Growth differentiation factor 5 (GDF5) molecules are attached to the nanoparticles. The method further comprises the steps of adding a first differentiation medium to the seeded iPSCs and allowing the seeded iPSCs to differentiate at least into chondrocyte progenitor cells on the surface in the presence of the first differentiation medium. The obtained differentiated cells are integrated into a matrix/scaffold.

Methods of treating a complex wound by administering to a complex wound in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex wound. Methods of treating a complex lower extremity ulcer by administering to a complex lower extremity ulcer in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex lower extremity ulcer. Methods of reducing or preventing scar formation from granulation tissue by administering a fetal support tissue product to granulation tissue. Methods of repairing a spina bifida defect by administering to the defect in the individual a therapeutically effective amount of an umbilical cord product.