Described herein are RPE cells engineered to secrete a GLA protein, as well as compositions, pharmaceutical preparations, and implantable devices comprising the engineered RPE cells, and methods of making and using the same for treating Fabry disease.

A method for preparing a skin regeneration scaffold is disclosed. The method may include preparing a polymer solution by dissolving a biopolymer in a solvent, and subjecting the polymer solution to a template-assisted extrusion process with a nanoporous material as a template in order to produce polymer nanofibers. Furthermore, the method includes fabricating a multilayer composite nanofibrous scaffold using the polymer nanofibers. The composite nanofibrous scaffold may be seeded with cells. In some cases, the cells may be selected from autologous cells, allogeneic cells, or combinations thereof.

The presently disclosed subject matter provides hydrogel precursor compositions (e.g., solutions) for forming three-dimensional hydrogels that support growth of physiologically relevant tissue when at least one cell is cultured in the three-dimensional hydrogel, kits comprising the hydrogel precursor composition, three-dimensional hydrogels, methods of forming the three-dimensional hydrogels, methods of growing the physiologically relevant tissue using the three-dimensional hydrogels, physiologically relevant tissue grown in the three-dimensional hydrogels, methods of producing hormone-responsive tissue (e.g., milk-producing mammary tissue and related methods of producing milk), methods of screening for candidate agents useful for modulating hormonal responses (e.g., modulating milk production), method of screening for candidate therapeutic agents using the physiologically relevant tissue grown in the three-dimensional hydrogels (e.g., personalized cancer treatments), and related methods of treatment (e.g., administering agents identified using the methods herein, transplanting physiologically relevant tissue produced using the methods, etc.).

The present invention is directed to a method of producing a tissue graft, comprising at least steps of providing a gel, seeding the gel with cells of at least a first and/or cells of a second type, and culturing of the cells of the first and/or cells of the second type in said gel until the formation of at least one first biostructure in the gel by the cells of the first type and/or the cells of the second type.

A method of simulating a biological response of a cellular system may include removing at least some DNA-containing material from a vascular plant tissue to produce a vascularized cellulose scaffold, seeding the vascularized cellulose scaffold with cultured biological cells, growing cultured biological cells on the vascularized cellulose scaffold to produce the vascularized biological system, subjecting the vascularized biological system to an external stimulus, and measuring a response of the vascularized biological system. In some embodiments, the removing step comprises submerging the plant tissue in a fluid comprising supercritical CO2, peracetic acid and ethanol.

The specification relates to a method of manufacturing a diabetic foot patient-specific skin regeneration sheet, and a diabetic foot patient-specific skin regeneration sheet.

The present disclosure aims to provide a manufacturing method of a cell structure. The manufacturing method comprises a preparation step of preparing, on a culturing surface of a cell culture container, a first coated region coated with a temperature-responsive polymer and/or a temperature-responsive polymer composition, and a plurality of second coated regions located at an edge of the first coated region and coated with a cell adhesive substance; and a seeding and culturing step of seeding cells in the first coated region and the second coated regions and culturing the cells to produce a cell structure.

This invention is directed to structural units and bioscaffolds that comprise the same for in vivo use.

Proposed is a core-shell structure including a shell portion and a core portion, in which the shell portion includes n shells that are sequentially located from outside to inside, the core portion includes a core located inside the shell portion, n is any one of natural numbers from 1 to 30, when n is 1, the core is located adjacent to the inside of a first shell, when n is any one of natural numbers from 2 to 30, an n.sup.th shell is located adjacent to the inside of an n−1.sup.th shell, the n.sup.th shell is an empty space or is a hydrogel including at least one of an n.sup.th extracellular matrix and an n.sup.th cell, the core is an empty space or is a hydrogel including at least one of an extracellular matrix for a core and a cell for a core, two of the n shells and the core that are in contact with each other are not empty spaces simultaneously, and densities of the two of the n shells and the core that are in contact with each other are identical or different, thereby mimicking the construction of hollow organs such as the stomach, intestines, bladder, and lungs.

To provide means for efficient growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs. Provided are a culture medium for growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs, the culture medium comprising basal medium and at least (1) through (3), below: and a method for growing the epithelial cells using the culture medium: (1) at least one species of BMP inhibitor: (2) at least one species of fibroblast growth factor: and (3) at least one species of sonic hedgehog (SHH) and/or SHH agonist.