The present application relates to the manipulation and handling of biological materials and, in one form, provides an apparatus for micromanipulation of biological material, including a channel for accommodating biological material and allowing for passage of liquid treatment solutions. The apparatus may include a two part construction wherein two portions of the apparatus are adapted to be heat sealed with a secondary material intermediate the two portions prior to a vitrification process step. A system for vitrification of a biological specimen is also provided including a software operable means for controlling the temperature environment, a software operable means for controlling fluid dispense volume and velocity and aspiration volume and velocity for the application of liquid treatment solutions to the biological specimen, and a software operable means for controlling protocol time.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.

The invention relates to a machine for labeling “blank-labeled” cryogenically-frozen vials or ampoules, which contain heat-labile biological materials, and to which a laser-light sensitive material had been applied prior to freezing. Accordingly, the machine has been designed to maintain the integrity of the biological materials throughout all phases of the labeling process. The machine generally comprises a master control system; a programmable user interface; a frame; cryogenic freezer assemblies, for keeping the vials at the required low temperatures; an infeed assembly, configured to receive and position blank-labeled cryogenic vials; a cryostatic labeling/quality control tunnel, wherein the vials are maintained at the required temperature, labeled by laser ablation, and checked for quality; and, an outfeed assembly. The machine further comprises a means for transporting the vials from the infeed assembly to the tunnel, and from the tunnel to the outfeed assembly. Vials labeled according to the instant disclosure are ultimately manually or automatically loaded into cryogenic shipping containers.

Containers (100) for cryopreserved biological samples (102) may include an insulated housing including a cavity (108) for containing at least one cryopreserved biological sample; and a sealed reservoir (106) at least partly surrounding the cavity, the sealed reservoir including liquified gas (120) such as liquified air, the gas being kept largely liquified by a heat transfer engine (112) such as a Stirling cryocooler. A valve (114) may be provided to function as both a pressure relief valve and an inlet valve. The inlet valve may be coupled to a sensor (122) for sensing a volume of liquified gas within the sealed reservoir. The container may further include a heat exchanger (116) coupled to the heat engine and extending into the sealed reservoir.

A high pressure freezing cartridge (300, 400, 500) for use in vitrification of a biological sample (10) is provided, the cartridge (300,400, 500) being adapted to fixedly hold a sample chamber (216, 218, 220) at a sample chamber position (320, 420, 520) in the cartridge (300, 400, 500), a refrigerant channel arrangement comprising at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) being provided in the cartridge (300, 400, 500) and extending from a surface of the cartridge (300, 400, 500) to the sample chamber position, and the cartridge (300, 400, 500) comprising a baffle structure (350, 450, 550, 571) at a baffle position being adapted to interact with a refrigerant stream in the refrigerant channel arrangement before the refrigerant of the refrigerant stream reaches the sample chamber position in the cartridge (300, 400, 500). A corresponding method in which the high pressure freezing cartridge (300, 400, 500) is used is also part of the present disclosure.

A freezer includes a plurality of shelves in an insulated payload bay; a plurality of evaporators coupled to the payload bay with a multiplicity of coolant tubes in each evaporator, wherein each tube enters and then exits the payload bay, further comprising one or more cryogenic valves coupled to the coolant tubes; a pump to force coolant flowing through the evaporators with a pressure of at least 90 psi to supply the coolant at each evaporator with at least 20 gallons per hour of coolant; and a plurality of fans to circulate cooled air in the payload bay.

This invention relates to a novel kit, transportable apparatus and method for generating in-situ CO2 snow block within the apparatus. An item such as a biological sample can be stored and transported within the same apparatus that is employed for creating the CO2 snow block. The apparatus is capable of preserving the sample during transport. The invention also includes a specially designed CO2 snow charger system including a charger and meshed conduit. The charger system is operated in accordance with the methods of the present invention to create the in-situ CO2 snow block within a container that can be also used for transport.

An object of the present invention is to provide a freezing transport container which can store large samples such as bag shapes and allows easy removal and installation of a cryogenic liquefied gas absorber which is impregnated with cryogenic liquefied gas such as liquid nitrogen, and a cryogenic liquefied gas absorber case which can be installed in the freezing transport container, and the present invention provides a freezing transport container (1) including a main body (3) which has a heat-insulating structure, an opening (3a) at the top, and a bottomed tubular shape, and a lid (5) which is installed at the opening (3a) of the main body (3) so as to open and close the opening (3a) of the main body (3), wherein the main body (3) has the same diameter at the opening (3a) at the top and the bottom, the freezing transport container (1) further includes a cryogenic liquefied gas absorber case (7) which is installed removably at the bottom of the main body (3), and the cryogenic liquefied gas absorber case (7) includes a case portion (11) which has a bottomed tubular shape and a cryogenic liquefied gas absorber (13) which is installed replaceably inside the case portion (11).

A cryogenic storage system provides automated storage and retrieval of samples in a cryogenic environment, as well as automated transfer of individual samples between cryogenic environments. Stored samples are maintained under a cryogenic temperature threshold, while also enabling access to the samples. The samples are organized and tracked by scanning a barcode of each sample. Embodiments may also comprise multiple storage vaults and provide for transfer of individual samples between the storage vaults, as well as between a storage vault and a removable cryogenic storage device.

The present disclosure provides systems and methods for use in freezing liquid mixtures or suspensions containing sensitive substances, such as biopharmaceutical materials, under sterile conditions and in small-volume containers. The disclosed device enables the control of ice nucleation of the solution minoring the layer of volume that freezes, while controlling the ice growth rate in a bottom up geometry, and comprises a heat transfer surface (101) with means to control temperature, a holder (102) for multiple containers (109), pressing means (103) to press the holder against the heat transfer surface and optionally a contact promoting material. The disclosed method comprises pre-cooling the device to a temperature substantially below the solution nucleation temperature, placing a container into the holder, contacting the container with the heat transfer surface until a fraction of 10% of the total sample volume is frozen; interrupting the contact between the container and the heat transfer surface; contacting the container with the heat transfer surface at a predefined freezing rate, such that the freezing of the biological solution is homogeneous; until all the volume of the solution is frozen.