A tissue scaffold includes a first film having a plurality of cell openings and a second film adjacent the first film and having a plurality of cell openings larger than the cell openings of the first film. The cell openings of the first film interconnect with the cell openings of the second film to define pathways extending through the first and second films.

A shaped cartilage matrix isolated from a human or animal where the cartilage has been crafted to facilitate disinfection, cleaning, devitalization, recellularization, and/or integration after implantation. Also, a process for repairing a cartilage defect with the cartilage matrix. The matrix is in the form of an osteochondral plug including a cartilage cap ad subchondral bone, wherein one or more gaps, slats, bores, or channels extend through the tidemark at the interface between the cartilage cap and the subchondral bone.

Devices and methods for in situ drawing, filtering and seeding cells from the marrow of surrounding bone into a fusion cage without any of the challenges mentioned above. Various implants and devices with aspiration ports that enable in-situ harvesting and mixing of stem cells. These devices may include spinal fusion cages, long bone spacers, lateral grafts and joint replacement devices. Each device utilizes at least one aspiration port for harvesting of stem cell-containing marrow via aspiration from adjacent bony elements.

Devices and methods for in situ drawing, filtering and seeding cells from the marrow of surrounding bone into a fusion cage without any of the challenges mentioned above. Various implants and devices with aspiration ports that enable in-situ harvesting and mixing of stem cells. These devices may include spinal fusion cages, long bone spacers, lateral grafts and joint replacement devices. Each device utilizes at least one aspiration port for harvesting of stem cell-containing marrow via aspiration from adjacent bony elements.

A tissue scaffold includes a first film having a plurality of cell openings and a second film adjacent the first film and having a plurality of cell openings larger than the cell openings of the first film. The cell openings of the first film interconnect with the cell openings of the second film to define pathways extending through the first and second films.

A tissue scaffold includes a first film having a plurality of cell openings and a second film adjacent the first film and having a plurality of cell openings larger than the cell openings of the first film. The cell openings of the first film interconnect with the cell openings of the second film to define pathways extending through the first and second films.

Disclosed herein is a modified tissue graft (120, 200, 300, 500, 630) with improved properties for tissue integration and regeneration. The tissue graft (120, 200, 300, 500, 630) has biological properties that are enhanced by removing lipids (615) and other contaminants (e.g., blood components, micro-organisms) using enzymes, enzyme aggregates, or immobilized enzymes. This application describes the process (10) for removing bio-contaminants while maintaining optimal biological properties.

Devices and methods for in situ drawing, filtering and seeding cells from the marrow of surrounding bone into a fusion cage without any of the challenges mentioned above. Various implants and devices with aspiration ports that enable in-situ harvesting and mixing of stem cells. These devices may include spinal fusion cages, long bone spacers, lateral grafts and joint replacement devices. Each device utilizes at least one aspiration port for harvesting of stem cell-containing marrow via aspiration from adjacent bony elements.

An assembly for preparing bone stock includes a shell (52) comprising a base (54) having a support surface (56), and a lid (58). The base and lid have a void space (60) therebetween. A carriage (62) is disposed at least partially within the void space, and moveable across the support surface. The carriage comprises a wall (64), at least a portion (65) of which is resiliently deformable. The wall cooperates with the base to define a preparation chamber (66), and a removal element (68) configured to remove soft tissue from the bone stock is disposed at least partially therein. The resilient portion of the wall is configured to deform when bone stock is positioned between the removal element and the wall. The carriage is configured to receive power from a drive train to actuate movement of the carriage across the support surface, and the removal element is also configured to receive power from the drive train.

An assembly for preparing bone stock includes a shell comprising a base having a support surface, and a lid. The base and lid have a void space therebetween. A carriage is disposed at least partially within the void space, and moveable across the support surface. The carriage comprises a wall, at least a portion of which is resiliently deformable. The wall cooperates with the base to define a preparation chamber, and a removal element configured to remove soft tissue from the bone stock is disposed at least partially therein. The resilient portion of the wall is configured to deform when bone stock is positioned between the removal element and the wall. The carriage is configured to receive power from a drive train to actuate movement of the carriage across the support surface, and the removal element is also configured to receive power from the drive train.