The present invention generally relates to cosmetic compositions comprising serum derived from cultured keratinocyte and fibroblast cells. The invention also relates to methods of producing serum for use in the cosmetic compositions through novel cell culture techniques.

A multi-purpose moisturizer composition. In one embodiment, the multi-purpose moisturizer has the form of a cream and is composed of a plurality of butter compounds such as, for example, rhea butter, cocoa butter, and jojoba butter, combined with a plurality of essential oils such as, for example, sweet almond oil, castor oil, argan oil, coconut oil, grape seed oil, and the like. In another embodiment, the multi-purpose moisturizer has the form of an oil and is composed of a plurality of essential oils such as, for example, hemp seed oil, vitamin E oil, chamomile oil, and the like. The cream or the oil embodiments of the multi-purpose moisturizer may be applied to an area of dry skin in order to alleviate itching and irritation while returning the skin to a healthy moisture level.

Hair cleansing rinses which contain based on the weight of the total compositions: a) from about 0.10 to about 30% by weight of at least one amphoteric surfactant, b) from about 0.10 to about 15% by weight of at least one cationic surfactant of the quaternary ammonium compound type, c) from about 0.01 to about 15% by weight of at least one hydrophobically modified starch compound and d) from about 0.01 to about 2.00% by weight of at least one protein hydrolyzate have an excellent cleaning and conditioning effect.

Hair treated with these hair cleansing rinses has luster and resilience even after repeated use. There is no buildup effect on the hair. In addition, hair treated in this way has allows better distribution and rinse out than traditional hair conditioning compositions.

A biological hair shape change composition including a macromolecular component, a small molecular component and an alkali agent component is provided. The macromolecular component includes a protease belonging to the class of alkaline proteases, and the small molecular component includes a peptide with reduction activity. Both the macromolecular component and the small molecular component are obtained from a fermentation product of Bacillus licheniformis and a keratin and/or keratin polymer-containing medium. Molecular weights of ingredients in the macromolecular component are greater than or equal to 3 kDa and are 3-1000 kDa, and molecular weights of ingredients in the small molecular component are less than 3 kDa and are 0.01-2.99 kDa.

There is provided a topical skin composition directed to one or more desired benefits. The composition includes one or more proteins selected based on the desired skin benefit to be derived by application of the topical skin composition. In one embodiment, each protein is from one donor group having two or more individuals. In another embodiment, the protein is from two or more donor group.

The present invention relates to a method for culturing skin-derived stem cells and uses of the same, particularly to a method for culturing skin-derived stem cells; skin-derived stem cells prepared by the method; and a pharmaceutical composition for skin regeneration or wound treatment; a quasi-drug composition; and a cosmetic composition, which contain the prepared skin-derived stem cells, a culture of the skin-derived stem cells, or cells differentiated from the skin-derived stem cells as an active ingredient.

According to the culture method of the present invention, stem cells can be effectively isolated and obtained from skin tissue, and the obtained skin-derived stem cells have excellent pluripotency and self-replication ability as well as excellent skin regeneration and wound treatment effects and can be usefully utilized for skin regeneration and wound treatment or improvement.

The present invention relates to a method for culturing hair follicle-derived stem cells and uses thereof, particularly to a method for culturing hair follicle-derived stem cells; hair follicle-derived stem cells prepared by the method; and a pharmaceutical composition for hair loss treatment or hair growth promotion; a quasi-drug composition; and a cosmetic composition, which contains the prepared hair follicle-derived stem cells, a culture of the hair follicle-derived stem cells, or cells differentiated from the hair follicle-derived stem cells as an active ingredient.

According to the culture method of the present invention, stem cells can be effectively isolated and obtained from hair follicle tissue, and the obtained hair follicle-derived stem cells have excellent pluripotency and self-replication ability as well as excellent hair loss treatment and hair growth promotion effects and can be usefully utilized for hair loss improvement, prevention, or treatment.

A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.

Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

The present invention refers to a method of obtaining a composition comprising a cell suspension comprising hair progenitor cells (HPCs) from a human subject and plasma derived growth factors, which comprises the following steps: a. Incubating an isolated tissue sample comprising at least one complete hair follicle from the subject, preferably from the occipital region of the head of the subject, in a suspension of plasma derived growth factors, preferably obtained from the subject; for a period of time between 15 minutes and 1 hour at a temperature of approximately 37 C.; and b. Removing the tissue fragments from the product of step a); preferably by filtration; wherein in this method the cells from the tissue obtained from the sample of step a) are not enzymatically digested. The present invention also refers to a cell suspension or composition produced by the method thereof as well as the uses of such composition or cell suspension.