A porous membrane for use in an electroosmotic pump for pumping a fluid by electroosmotic transport, the porous membrane comprising: first and second opposite surfaces and a net fluid flow direction extending in the porous membrane between said opposite surfaces, wherein when a given amount of charge flows through the porous membrane from the first to the second opposite surface more electroosmotic transport of the fluid will occur than when the same amount of charge flows through the porous membrane from the second to the first, opposite surface.

A vacuum assembly includes a housing, a movable assembly positioned within the housing, and a biasing mechanism coupling the movable assembly to the housing. The movable assembly is movable between a first position and a second position within the housing to create a low pressure area between the housing and a first portion of the movable assembly.

A microporous structure includes an array of nano wires and a coating about the nano wires of the array. The coating defines pores between the nano wires.

A cassette comprising: a first reservoir arranged to couple to a first actuator for imparting flow to a cell-containing solution in a first direction through a filter; a second reservoir arranged to couple to a second actuator for imparting flow to a cell-containing solution in a second direction through the filter; the filter being in fluid communication with the first and second reservoirs, and when connected to a filtration unit is in further fluid communication to a sample inlet, and a sample outlet; wherein, the cassette is disposable and housed by a connector being removeably adjoinable to the filtration unit, the filtration unit comprising the first and second actuators and one or more valves.

A compact hydraulic manifold for transporting shear sensitive fluids is provided. A channel network can include a trunk and branch architecture coupled to a bifurcation architecture. Features such as tapered channel walls, curvatures and angles of channels, and zones of low fluid pressure can be used to reduce the size while maintaining wall shear rates within a narrow range. A hydraulic manifold can be coupled to a series of microfluidic layers to construct a compact microfluidic device.

Devices and methods integrate nanopore and microfluidic technologies for recording molecular characteristics of individual molecules such as, for example, biomolecules. Devices comprise a first substrate comprising a microchannel, a second substrate comprising a microchannel, the second substrate positioned below the first substrate, and a membrane having a thickness of about 0.3 nm to about 1 nm and comprising at least one nanopore, the membrane positioned between the first substrate and the second substrate, wherein a single nanopore of the membrane is constructed and arranged for electrical and fluid communication between the microchannel of the first substrate and the microchannel of the second substrate. To mitigate the effect of errors that occur during de novo DNA synthesis, longer DNA molecules are typically synthesized from shorter oligonucleotides by polymerase construction and amplification (PCA), or by other methods.

A micro fluid filtration system (100) preferably for increasing the concentration of components contained in a fluid sample has a fluid circuitry (1). The fluid circuitry (1) comprises the following elements: A tangential flow filtration element (7) capable for separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid, an element for pumping (3) for creating and driving a fluid flow through the fluid circuitry (1) and at least one element for obtaining information about the properties of the fluid sample within the circuitry. The circuitry further comprises a plurality of conduits (24) connecting the elements of the fluid circuitry (1) through which a fluid stream of the fluid sample is conducted. The circuitry (1) has a minimal working volume of at most 5 ml, which is the minimal fluid volume retained in the elements and the conduits (24) of the circuitry (1) such that the fluid can be recirculated in the circuitry (1) without pumping air through the circuitry (1). An integrated microfluidic element (20) of the circuitry (1) contains the functionality of at least two elements of the group of elements of the circuitry (1).

A method for automated processing of a blood product, the method comprising providing a reusable separation apparatus controlled by a microprocessing unit, said apparatus configurable with settings and configured to associate with a disposable circuit comprising a separator and in communication with a source blood product having a first concentration and first volume. The apparatus and disposable circuit are configured to flow the source blood product into an inlet of the separator and separate supernatant of the source blood product from a first outlet of the separator into a filtrate container. The apparatus and disposable circuit are also configured to separate platelets and remaining supernatant from a second outlet of the separator into a retentate container, wherein the platelets and remaining supernatant in the retentate container have a second concentration greater than the first concentration and second volume less than the first volume.

The present invention discloses a method to continuously manufacture micro- and/or nanoparticles of single component particles or multi-component particles such as particulate amorphous solid dispersions or particulate co-crystals. The continuous method comprises the steps of 1. preparing a first solution comprising at least one component and at least one solvent and a second solution comprising at least one anti-solvent of the at least one component comprised in the first solution, 2. mixing said first solution and said second solution by means of microfluidization to produce a suspension by precipitation or co-precipitation, 3. feeding said suspension to a filtration system to obtain a concentrate stream, 4. feeding said concentrate stream to a spray dryer, 5. atomizing said concentrate stream using at least one atomization nozzle, 6. drying said atomized concentrate stream to obtain particles, and 7. collecting said particles. Single component particles or multi-component particles, particulate amorphous solid dispersions, particulate co-crystals and pharmaceutical compositions are also disclosed.

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel (30, 60) of an upstream microfluidic device (1). Target cells present in the biological sample are captured in cell channels (20) of the upstream microfluidic device (1). Once at least a minimum number of target cells are captured in the cell channels (20), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device (1) to release the target cells captured in the cell channels (20) of the upstream microfluidic device (1) and enable transfer the target cells into cell channels (120) of a downstream microfluidic device (100).