A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the spinning membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib or ridge associated with the spinning membrane that decreases the gap between the spinning membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously-collected whole blood into selected blood components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed.

Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.

The present invention directs to a centrifugal filtration device, for separating living cells, including a spindle (28), a rotary arm (211) which is connected vertically to the spindle (28), a microporous membrane filter (31A;31B) which is mounted on the rotary arm (211), and a liquid distributor (4) arranged above the spindle (28). The microporous membrane filter (31A;31B) includes an inlet (311A;311B), an outlet (312A;312B), a front cavity (313A;313B) having the inlet (311A;311B) formed thereon, a rear cavity having the outlet (312A;312B) formed thereon, and a filter membrane (315A;315B) arranged between the front cavity and the rear cavity; the diameter of each filter pore formed in the filter membrane is smaller than that of the cell which needs to be separated. The present invention also discloses a cell separation system.

A method of operating a high velocity cross flow dynamic membrane filtration includes feeding a fluid stream into a pressure vessel, in which the vessel defines a treatment chamber containing a disc membrane assembly having a first support shaft and a second support shaft, each support shaft defining a longitudinal axis about which is positioned a plurality of axially spaced membrane discs. The method further includes distributing the fluid stream over at least a portion of the disc membrane assembly. The method also includes discharging a first portion of the fluid stream from the vessel and discharging a second portion of the fluid stream from the vessel. The method additionally includes rotating the first support shaft and the second support shaft in a first direction. The rotating includes modulating a rotation rate in response to the flow rate of the second portion of the fluid stream.

A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the spinning membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib or ridge associated with the spinning membrane that decreases the gap between the spinning membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously-collected whole blood into selected blood components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed.

A membrane separation apparatus includes: a shell, wherein an inner surface of the shell is an arc surface, and at least one medium inlet and at least one medium outlet used for discharging a medium that is separated are arranged on the shell; a rotor arranged inside the shell, wherein at least two contact ends that are always in slidably contact with the inner surface of the shell are arranged on an outer surface of the rotor, the outer surface of the rotor and the inner surface of the shell form sealed separate cavities between the adjacent contact ends, and an empty part inside the rotor is used as a medium storage chamber; and separation chambers arranged inside the rotor.

Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.

A mitochondria extraction apparatus provided in the present invention includes a container used for holding a mixture fluid and a diafiltration assembly disposed in the container and used for isolating mitochondria. A basement of the mitochondria extraction apparatus is disposed in the container. During a circular motion of the mitochondria extraction apparatus, a centrifugal force in the mitochondria extraction apparatus acts on the mixture fluid in a hole-shaped microfluidic channel included in the diafiltration assembly, so as to provide a pushing force for the mixture fluid, enabling the mixture fluid to rapidly flow in the microfluidic channel for diafiltration.

A cell filtration assembly adapted to capture cells from a biological sample during centrifugation includes a centrifugation tube, a cell collection device adapted to be secured within a tapered end of the centrifugation tube and a funnel structure adapted to direct fluid into the cell collection device. The cell collection device includes a rectilinear structure that is adapted to permit fluid to flow through the rectilinear structure and a cell capture surface that is secured relative to the rectilinear structure. The cell collection device may be sectioned in either a horizontal or vertical orientation.

The present invention relates to a filtration device comprising: at least one enclosure (1; 1A, 1B; 1A, 1B, 1C, 1D) defining a longitudinal axis, said enclosure being obstructed at each end by at least one sealing plate (2A, 2B; 2C, 2D), at least one filtration disc (4) that is rotated and at least one spacer (10) placed between each filtration disc (4), said spacer (10) defining an inter-disc space (10A), at least one hollow rotation shaft (3; 3A) that rotates said at least one filtration disc (4), said shaft having at least one port (33) adapted to collect filtrate (11A, 11B), said filtration disc (4) and said spacer (10) being arranged on said at least one rotation shaft (3; 3A, 3B) inside said enclosure (1; 1A, 1B; 1A, 1B, 1C, 1D), characterised in that said enclosure (1; 1A, 1B; 1A, 1B, 1C, 1D) is passed through by said at least one rotation shaft (3; 3A), and said rotation shaft (3; 3A) is driven by at least one separate rotation means (5, 5A, 5B, 5C) on at least one of the ends of said shaft, said rotation means and said rotation shaft being coaxial, and in that the device comprises at least two separate discharge means (13A, 13B) for the filtrate (11A, 11B), said discharge means being located on said rotation shaft outside said enclosure.