The present disclosure relates to the field of making high molecular weight adenosine-based coenzymes available on a large scale. In particular, it relates to a method for purifying high molecular weight adenosine-based coenzymes by implementing a tangential diafiltration, or even dia-ultrafiltration step. This method is, for example, applicable to the purification of coenzyme A disulfide ((CoAS).sub.2), coenzyme A (CoA), nicotinarnide adenine dinucleotide (NAD+), nicotinarnide adenine dinucleotide phosphate (NADP.sup.+) or flavin adenine dinucleotide (FAD).

The present invention provides a process for the production of a protein powder having an improved solubility and taste profile from brewer's spent grain. The process comprises nanofiltration at a specified applied pressure. The present invention also provides a protein powder produced from brewer's spent grain, a process for producing food or beverage products incorporating the protein powder, and food or beverage products comprising the protein powder.

A process for purifying a liquid feedstock comprising a monoclonal antibody and impurities, the process comprising passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified monoclonal antibody and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v) which include a multiple inlet flow-controller comprising two or more variable flow inlet valves for in situ production of a bioprocessing liquid by combining at least two liquids in a desired ratio. One of the units performs chromatography and another performs viral inactivation. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

A crystalline pigment or colorant composition having high color intensity and/or low sugar content, and methods and processes of preparation. The composition may comprise purified fruit and/or vegetable color juices.

A multiple tangential flow filtration (TFF) apparatus includes a plurality of tangential flow filtration (TFF) systems, a support frame defining a plurality of stations for supporting individual (TFF) systems and a single controller for controlling the plurality of tangential flow filtration systems. Each of the systems include at least a product vessel, a tangential flow filtration (TFF) filter and a pump for circulating a flow of liquid between said product vessel and said filter.

The present invention provides compact spiral-wound filter elements having cassette-like performance. The invention further provides filtration systems (e.g., TFF systems) and processes (e.g., SPTFF processes) employing compact spiral-wound filter elements having cassette-like performance.

The present invention provides a method to isolate native tuber protein using a pretreatment of tuber processing water and diafiltration against a salt solution. This sequence of steps has the advantage that protein is stabilized during diafiltration, increasing process efficiency and protein quality and yield.

Disclosed is method for the purification of an oligosaccharide of interest from a fermentation broth, the method comprises providing a cell-free fermentation broth to a first filtration step using a nanofiltration membrane, thereby providing a filtrate which contains the oligosaccharide of interest; subjecting the filtrate to a second filtration step using a nanofiltration membrane, thereby providing a retentate which contains the oligosaccharide of interest; and removing salts from the retentate thereby providing a purified preparation of the oligosaccharide of interest.

The present invention relates to processes for obtaining a highly concentrated antibody solution. In particular, to processes for obtaining a highly concentrated therapeutic antibody solution that may be used for highly concentrated therapeutic antibody formulations, e.g. suitable for subcutaneous administration.

A method for degassing a cross-flow diafiltration unit, a cross-flow diafiltration method and a cross-flow diafiltration unit. The degassing method includes (i) providing a cross-flow diafiltration unit having a diafiltration channel, a retentate channel and a permeate channel, a first filter material that delimits the diafiltration channel from the retentate channel, and a second filter material that delimits the retentate channel from the permeate channel; and thereafter (ii) feeding a liquid into the retentate channel so that the liquid flows in a flow direction through the retentate channel and penetrates through the first filter material into the diafiltration channel, whereby the retentate channel and the diafiltration channel are filled with and degassed by the liquid