A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a κ-casein and at least one of an αS.sub.1-casein, an αS.sub.2-casein, a β-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the κ-casein, an inner matrix of the recombinant micelle include at least one of the αS.sub.1-casein, the αS.sub.2-casein, the β-casein.

A manufacturing facility for the continuous production of biopharmaceuticals integrated with single-use disposable technology.

Provided herein are methods of processing a fluid that include the use of one or both of a circuit system including a tangential flow filtration (TFF) unit and a circuit system including a tangential flow virus filtration (TFVF) unit. Also provided are integrated and continuous processes for manufacturing a therapeutic protein drug substance that include a step of processing a fluid including the recombinant therapeutic protein using any of the methods provided herein.

A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a -casein and at least one of an S.sub.1-casein, an S.sub.2-casein, a -casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the -casein, an inner matrix of the recombinant micelle include at least one of the S.sub.1-casein, the S.sub.2-casein, the -casein.

A zero-sugar and zero-net carb dairy product includes a washed fermented dairy material. Separation technology for the removal of sugars and organic acids from a fermented dairy material to produce the zero-sugar and zero-carb dairy product includes the repetition of a wash cycle involving a dilution step and a separation step. The separation technology achieves at least an 60% reduction in total sugars, at least an 80% reduction in organic acids and at least a 60% reduction in total carbohydrates. The zero-sugar and zero-net carb dairy product has a protein to carbohydrate ratio at least double that of conventional low-carb dairy products.

A dairy product that is fruit-flavored and reduced in sugar includes a washed fermented dairy material and a washed fruit-based material. Separation technology for the removal of sugars from a fermented dairy material and a fruit-based material to produce the dairy product includes the repetition of a wash cycle involving a dilution step and a separation step. The separation technology achieves at least a 10% reduction in total sugars.

A tangential flow filtration (TFF) system and disposable TFF unit that includes an integrated pump apparatus is disclosed. Namely, a TFF system is provided that includes a clamp assembly for integrating together a disposable TFF unit, two fluid reservoirs, and an air supply assembly. Further, the disposable TFF unit includes a diaphragm pump assembly and a concentration membrane. Further, methods of using the TFF system that includes the disposable TFF unit are provided.

Provided are a plate-type filter module for water treatment and a filter assembly for water treatment comprising the same. The plate-type filter module for water treatment according to one embodiment of the present invention comprises: a plurality of filter units having a predetermined area and formed in a plate shape; a block frame to which the plurality of filter units is engaged so that the filter units are kept parallel to each other; and a collecting/receiving tube fixed on one side of the block frame and including a plurality of first fittings respectively connected to the plurality of the filter units to allow filtered water produced in the filter units to be introduced thereinto and at least one second fitting for discharging the filtered water introduced through the plurality of first fittings to the outside.

The present disclosure relates to an apparatus and an apparatus assembly for assessing the suitability of a membrane, such as an osmotic membrane in an osmotic process. Furthermore, the apparatus, and apparatus assembly provide the means to optimise the parameters of an osmotic process, including the ability to gather key data for different membrane based osmotic systems to enable osmotic process and system optimisation.

The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing applications, systems employing such filters, and methods of filtration using the same.