A system for TFF filtration of a feedstock includes a feed vessel containing a solution comprising a target biomolecule, a filter membrane for filtering the solution, a feed pump for moving the solution from the feed vessel to the filter membrane, a buffer vessel coupled to the feed vessel by a buffer supply line, and a diafiltration pump disposed in the buffer supply line for delivering a buffer solution to the feed vessel. A variable path-length instrument is coupled to the feed line for determining, in real time, a concentration of the target biomolecule in the solution. A controller is coupled to the variable path-length instrument, the feed pump, and the diafiltration pump to control operation of the system based on the received information.

The present invention provides an improved process for isolating 2,4-bis-(2,4-dihydroxyphenyl)-6-(4-methoxyphenyl)-1,3,5-triazine (DIOPAT) from an aqueous alkaline mixture M having a pH of 10 or more and comprising the 2,4-bis-(2,4-dihydroxyphenyl)-6-(4-methoxyphenyl)-1,3,5-triazine, 2,4-dihydroxybenzophenone, and aluminum salts, wherein the process comprises a nanofiltration step, a precipitation step, and a further filtration step.

A sealing case for a filtration cassette that reliably prevents leaks after assembly and installation into a filtration system. The sealing case fits on the filtration cassette and provides a fluid-tight seal between the sealing case and around at least one port in the filtration cassette when installed in a filtration system. The sealing case may comprise two halves that mate together, and each half may be identical. The internal surface of the sealing case is flat or substantially flat.

Embodiments of the present application relate to commercial manufacturing processes for making bupivacaine multivesicular liposomes (MVLs) using independently operating dual tangential flow filtration modules.

This specification describes a membrane aerated biofilm media and reactor (MABR) having a discontinuous layer of a porous material applied to the outer surface of a gas-transfer membrane. The porous material may have a void fraction of 50% or more. The porous material may have a thickness of up to about 500 microns and a pattern on the same order of magnitude as its thickness. The media may be used to carry on a deammonification reaction. In use, ammonia oxidizing bacteria (AOB) and annamox bacteria grown in or on the media, with the annamox bacteria located primarily in the porous material. The supply of oxygen through the gas-transfer membrane is limited to suppress the growth of nitrite oxidizing bacteria (NOB). Excess biofilm is removed, for example by coarse bubble scouring. The media may be placed in an anoxic zone of an activated sludge plant, which may be upstream of an aerobic zone.

The present invention relates to a process for conditioning a raw suspension comprising at least one metal-organic framework and at least one suspension medium by means of at least one membrane filtration to obtain a product suspension. The invention relates also to a method, wherein said product suspension is coated to at least part of the surface of a substrate.

The proposed prefabricated filtration module is provided for a modular filtration system, in particular a cross-flow filtration system, for low-volume screening applications. The prefabricated filtration module includes fluid ports and a plurality of components adjusted to low-volume screening applications, which are firmly integrated into the filtration module. The entire filtration module is designed as a single-use filtration module.

This invention relates to a method for the enzymatic synthesis of oligosaccharides, preferably human milk oligosaccharides (HMOs) The method comprises the enzymatic transfer of a glycosyl moiety and subsequent removal of by-products, such as lactose, by nanofiltration using a membrane comprising an active polyamide layer.

A chromatography device is provided comprising a filter housing having an inlet and an outlet and defining a fluid flow path between the inlet and the outlet; a porous filter arranged in the filter housing across the fluid flow path, the filter comprising first porous filter element; and a second porous filter element in contact with the first porous filter element, wherein the first porous filter element comprises at least one anionic exchange (AEX) layer, and the second porous filter element comprises at least one hydrophobic interaction (HIC) layer. A method of purifying nucleic acid using the device is also provided.

Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.