The present disclosure provides a static mixer for mixing two or more fluids together. The static mixer comprises a tubular body defining a mixing chamber in which spheroidal bodies are positioned. The spheroidal bodies are arranged sequentially within the mixing chamber, and their size and shape is selected so that they maintain a staggered formation. The tubular body can receive a flow of the two or more fluids at one end and convey them towards an opposite end where a mixed product can be provided. The arrangement of the spheroidal bodies can induce turbulence in the flow of the two or more fluids to mix them as they meander around the spheroidal bodies. The present disclosure further provides a method for mixing two or more fluids by the static mixer.

Methods for mixing fluids including etanercept include providing a tank having an impeller with a 10 inch blade diameter disposed therein, filling a tank with a liquid composition comprising etanercept and one or more buffers to a level within the tank to cover the impeller, and operating the impeller to mix the liquid composition to a homogeneous state while maintaining a maximum shear stress imparted on the mixture of fluids at 125,000 s.sup.1 or below.

Methods for mixing fluids including etanercept include providing a tank having an impeller with a 10 inch blade diameter disposed therein, filling a tank with a liquid composition comprising etanercept and one or more buffers to a level within the tank to cover the impeller, and operating the impeller to mix the liquid composition to a homogeneous state while maintaining a maximum shear stress imparted on the mixture of fluids at 125,000 s.sup.1 or below.

A simulation device for generating a supersaturated total dissolved gas (TDG) in a flood discharge and energy dissipation region of a dam is provided. The simulation device includes a reactor, a water inlet system, a gas inlet system, a venting system, a water drainage and gas exhaust system, a monitoring system, and a control system. An observation window is defined on the reactor, and a magnetic stirring assembly is installed inside the reactor. The cooperation of the water inlet system and the gas inlet system quantitatively controls a mass flow rate of water and gas entering and exiting the reactor, controls the change and variation of a temperature and a pressure inside the reactor, simulates the process of generating supersaturated TDG in the flood discharge and energy dissipation region by injecting water flow with varying air entrainment concentrations, and simulates changes in supersaturated TDG concentrations under varying pressure conditions.

Disclosed herein are an agitation structure and an agitation method. The agitation structure includes an agitation inlet pipe and an agitation tank. The agitation tank includes a buffer pool and an agitation container. The agitation container is communicably installed below the buffer pool. The agitation inlet pipe is communicably installed on a side wall of the buffer pool. The agitation tank is provided with an agitator.

Disclosed herein are an agitation structure and an agitation method. The agitation structure includes an agitation inlet pipe and an agitation tank. The agitation tank includes a buffer pool and an agitation container. The agitation container is communicably installed below the buffer pool. The agitation inlet pipe is communicably installed on a side wall of the buffer pool. The agitation tank is provided with an agitator.

Miniature stirred tank bioreactor and system; the bioreactor configured for single use culturing, providing unobstructed culturing volume with sensors, probes and ports using minimally invasive placement in-wall or across the wall methods; the bioreactor further capable of containing scaffolds and features for culturing cells and tissue, including structures providing surfaces for cell growth, and screens for retaining microparticles during media exchange.