A container system includes a flexible bag bounding a chamber. A probe port includes an elongated tubular member having an interior surface bounding a first passageway and extending between a first end and an opposing second end, the first passageway being closed at the first end and open at the second end; and a flange encircling and radially outwardly projecting from the tubular member at or toward the second end thereof, the flange being secured to the flexible bag so that the first end of the elongated tubular member projects into the chamber of the flexible bag while the open second end of the elongated tubular member is accessible from outside of the flexible bag. A probe is received within the first passageway of the probe port.

A system for receiving a single-use vessel containing a mixing device has a support structure adapted to receive the single-use vessel and a drive-unit adapted to power, to control, or to power and control a mixing of contents of the single-use vessel when the single-use vessel is arranged in the support structure. The support structure and the drive-unit are provided as separate components that may be arranged both in an operating position, where the drive-unit is connectable to the mixing device of the single-use bag in the support structure, and in a separated position, where the drive-unit is separated from the support structure. The drive-unit comprises a tag reader that, in the operating position, is arranged to read a vessel tag of the single-use vessel arranged in the support structure.

A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.

A system for mixing content of a container comprises a container includes an interior mixing element, an exterior drive system and a mixing device comprising an exterior bearing for coupling the exterior drive system to the interior mixing element of the container. The exterior bearing provides a driven non-rotating, nutating motion that drives the interior mixing element of the container so that it performs a mixing motion inside the container.

The present invention relates to a mixing device having a stirring element that comprises: a container for receiving fluids and/or solids; and at least one rotatable stirring element for mixing the fluids and/or solids; wherein the stirring element comprises a first bearing element and a second bearing element which are arranged at or near opposite ends of the stirring element; wherein the first bearing element is mounted on a first face of the container and the second bearing element is mounted on an opposite second face of the container; wherein the first bearing element comprises at least one non-permanently magnetized element such that it can be moved in rotation by externally induced reluctance forces, and wherein the second bearing element is mounted in a contactless manner by externally induced magnetic forces. The invention also relates to a mixing device system.

A sparger assembly for a bioprocessing system includes a first layer having a plurality of pores of a first size, and a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size. The pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.

An impeller is usable in a variable diameter bioreactor having multiple vessel sections of successively increasing or decreasing volume. The impeller includes an impeller blade extending along an impeller blade axis between first and second axial ends and having opposed impeller blade faces, an impeller blade leading edge, and an impeller blade trailing edge. Each of the leading and trailing edges defines a helix or spiral between the axial ends of the impeller blade. In certain arrangements, the impeller blade is one of at least two impeller blades joined together along an impeller shaft extending axially along the impeller blade axis mentioned, and the helix or spiral has a pitch approximately equal to one half of a length between the first and second axial ends.

A bioprocess vessel includes a flexible bag or substantially rigid container that defines an interior volume and having a bottom surface, the bottom surface being open or containing an aperture therein for the passage of fluid. A pump is secured to the bottom surface of the flexible bag or substantially rigid container. In some embodiments is secured directly to the flexible bag or substantially rigid container. In other embodiments, the pump is secured indirectly the flexible bag or substantially rigid container using, for example, a port that extends through the aperture on the bottom surface. The port or flanged surface may also be integrated into the pump, which is secured to the vessel. An optional mixing adaptor may be provided inside the interior volume of the flexible bag or substantially rigid container and at least partially covers the inlet that leads to the pump.

Provided herein are apparatuses and systems for mixing liquids and suspensions that include vessels with structures that improve mixing while not contacting liquid delivery components. The apparatuses and systems can include a motor drive that allows speed and directional control of rotation of the vessel. The apparatuses and systems can include one or more magnets for separating magnetic beads in a suspension. Also provided are methods using said apparatuses and systems for mixing and separation processes.

A microscale bioreactor system for and method is disclosed for providing improved cell culture growth conditions in a small-volume vessel. For example, a microbioreactor system is provided that may include a small-volume vessel and wherein the small-volume vessel may include a field of actuatable surface-attached microposts. Further, the microbioreactor system may include an actuation mechanism for actuating the surface-attached microposts into movement. In some embodiments, the surface-attached microposts may be functionalized with, for example, activation signals for converting standard T-cells in a growth media to activated T-cells. Further, a method of using the microbioreactor system for providing cell culture growth conditions including enhanced oxygenation and nutrients distribution in a small-volume vessel is provided.