A nucleic acid amplification in-situ real-time detection system and method using a micro-fluidic chip through optical fiber sensing. The system includes a white light source, a detection optical path, a microfluidic chip and a spectrum acquisition, processing and display module, which are connected in sequence. The detection optical path is configured to transmit white light from the white light source to the micro-fluidic chip and transmit an optical signal made by the microfluidic chip to the spectrum acquisition, processing and display module. The micro-fluidic chip is configured to carry out biochemical reaction; the spectrum acquisition, processing and display module is configured to acquire the optical signal, analyze the signal and generate a visual biochemical reaction real-time dynamic-change signal curve. This microfluidic chip real-time detection device detects nucleic acid amplification information by using a white light interfered hyperspectral method, so fluorescence-labeled analyte and non-fluorescence-labeled analyte are detected.

The present disclosure, in some aspects, provides devices useful for determining a target nucleic acid profile of a sample. The device comprises an enrichment module, a reaction module and a nucleic acid sequencer. In other aspects, the present disclosure provides kits, components and compositions (such as consumables) of the devices and methods of using the devices described herein.

The present specification discloses a microfluidic analysis chip capable of adjusting movement of a specimen or a reagent by a negative pressure generation unit. A microfluidic analysis chip according to the present specification may comprise: a microtube for a main channel, which provides a space in which a specimen input through a specimen inlet formed at one end thereof reacts with a regent while the specimen moves to the other end thereof; a chip housing surrounding the microtube for the main channel; and a negative pressure generation unit which is positioned in the chip housing and connected to the microtube, so as to affect an internal pressure of the microtube for the main channel.

Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

The invention relates to a device (1) for analyzing biological samples (S) comprising a substrate (2) for receiving a biological sample (S), wherein the substrate (2) comprises or consists of a fibrous material (F) configured to form a stationary phase which retains molecules in a biological sample (S) dissolved in a liquid mobile phase depending on molecular weight and/or polarity of the molecules, a first electrode (41) and a second electrode (42), which are arranged along a first axis (A1) and configured to generate an electric field acting along the first axis (A1) when an electric potential difference is provided between the first electrode (41) and the second electrode (42), so that charged molecules contained in the biological sample (S) are movable through the substrate (2) along the first axis (A1) and/or separable by their molecular weight, their polarity and/or their charge, wherein the substrate (2) comprises a chemical lysing agent (L) capable of lysing the biological sample (S). Furthermore, a method for analyzing biological samples (S) using the device (1) is provided.

An in-vitro diagnostic analyzer, a reagent card (10), and an installation structure (200) are disclosed. The installation structure (200) includes an installation body (210). The installation body (210) includes an installation hole (212) configured to sleeve a sample tube (70), a hollow needle (220), a sealing portion (240), and an air inlet channel (230). One end of the hollow needle (220) is capable of being inserted into the sample tube (70). The sealing portion (240) is in sealing fit with an outer wall of the sample tube (70). The air inlet channel (230) includes an air outlet hole (234) and an air inlet hole (232). The air outlet hole (234) is configured for communication with the sample tube (70) provided on the installation hole (212). The reagent card (10) is integrated with the installation structure (200), and the in-vitro diagnostic analyzer is integrated with the reagent card (10).

A semiconductor sensor-based near-patient diagnostic system and related methods.

A 3D microfluidic device for use as an in vitro lymph node is described. The microfluidic device has a body with a semi-circular inner wall and a first channel located adjacent along the semi-circular inner wall, the first channel corresponding to a subcapsular sinus region of a lymph node, a second channel located adjacent the first channel, the second channel corresponding to a reticular network, and a bottom cavity and top cavity, centrally located, corresponding to a paracortex and follicle of a lymph node, respectively. The various compartments of the device are separated by circumferentially and horizontally located rows of micro-pillars. A lab-on-a-chip device incorporating the microfluidic device is also described.

The present disclosure relates to a rapid genetic screening method and device. The method includes: collecting a sample to be tested of a patient through a micro-fluidic chip, where the sample to be tested includes a whole blood or saliva or nasopharyngeal swab or wound swab sample of a patient; lysing and amplifying the sample to be tested in the micro-fluidic chip to obtain an amplified nucleic acid segment; fusing a biosensor with amplification liquid, where the biosensor is provided with a DNA probe which can only be bounded to a specific nucleic acid segment and in which an impedance may dramatically change before and after the bounding; and inputting an electrical signal to the biosensor, testing a signal of an output end, and determining whether a nucleic acid segment matched with the DNA probe exists in the sample to be tested of the patient. The DNA probe can be replaced to test whether different nucleic acid segments exist. A person only need to collect the sample to be tested of the patient, select a probe, and configure simple parameters, so that the operations are simple, without performing nucleic acid extraction and purification on the sample to be tested, and the testing efficiency is greatly improved.

A flow control and processing cartridge includes a cartridge body and a reaction chip. The cartridge body includes plural first chambers and plural first channels for storing and processing at least one of a sample, a reagent and a buffer and configured to perform nucleic acid extraction. The reaction chip is in conjunction with the cartridge body and includes plural second chambers and plural second channels configured to store and process an amplification reaction solution, and at least two fluidic networks configured to perform nucleic acid amplification and detection. One of the fluidic networks includes plural detection wells, a main fluid channel connected with the detection wells and configured to dispense the sample or control liquids into the detection wells, and a gas releasing channel connected with the detection wells and configured to release gas from the detection wells, wherein one of the fluidic networks is configured for quality control.