Single-use diagnostic consumables for use in performing multiple analyses on a fluid sample are provided. The diagnostic consumables include a first sensing region configured for analysis of at least one analyte in a fluid sample that has been received by the diagnostic consumable. The diagnostic consumable further includes a fluid transport material configured to flow a portion of the fluid sample into a second sensing region fluidically connected to the fluid transport material and configured for performing a second analysis of the fluid sample. Methods for performing multiple analyses of a fluid sample on a single-use diagnostic consumable are also provided.

A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

A microfluidic group includes a female connector and a male needle connector. The female connector has a connector chamber in a containment body; a duct extending in the containment body to a duct opening on a first face of the connector chamber; a needle entry hole extending from a lateral face of the containment body to a second face, not facing the first face of the connector chamber; and a gasket arranged in the connector chamber. The gasket has a side wall internally delimiting a cavity and extending in part adjacent to the second face of the connector chamber. The cavity of the gasket faces the first face of the connector chamber.

A microfluidic group includes a female connector and a male needle connector. The female connector has a connector chamber in a containment body; a duct extending in the containment body to a duct opening on a first face of the connector chamber; a needle entry hole extending from a lateral face of the containment body to a second face, not facing the first face of the connector chamber; and a gasket arranged in the connector chamber. The gasket has a side wall internally delimiting a cavity and extending in part adjacent to the second face of the connector chamber. The cavity of the gasket faces the first face of the connector chamber.

The present invention provides an extraction cassette, which includes an extraction module and a liquid receiving module. The liquid receiving module communicates with the extraction module. The liquid receiving module includes a receiving module body, a sample compartment, a sealing member, an alcohol compartment and a first path. The receiving module body includes a first side, a second side and a sample feeding hole. The sample compartment is formed on the receiving module body. The sample compartment communicates with the sample feeding hole, the sample compartment includes a first sample compartment connection hole and a second sample compartment connection hole. The sealing member is adapted to seal the sample feeding hole. The alcohol compartment is formed on the receiving module body. The alcohol compartment communicates with the first sample compartment connection hole of the first sample compartment via the first path.

Microfluidic devices and methods of quantifying fatty acids and/or specialized pro-resolving mediators and/or fatty acid metabolites present in a fluid sample on a microfluidic device are described herein. The methods include extracting fatty acid esters containing fatty acids from the fluid sample, combining the extracted fatty acid esters with a hydrolyzing agent to cleave the fatty acids from the extracted fatty acid esters and form free fatty acids, and quantifying the free fatty acids by performing a bioassay specific to the free fatty acids. Microfluidic devices and methods of quantifying fatty acid metabolites present in a fluid sample on a microfluidic device are also described herein.

Devices that may couple two or more apparatuses, such as an organ-on-a-chip device and a microfluidic device. Devices that include an organ-on-a-chip device, a microfluidic device, and a cap that couples the organ-on-a-chip device and the microfluidic device. Systems that include the devices and a detection unit. Methods for quantitation of insulin.

The present disclosure relates to a sampling and sensing device and a method of using the sampling and sensing device for collecting and sensing chemical or biological analytes. The sampling and sensing device comprises a handle and a foldable head having a sampling region and a sensing region. A sensor chip may be disposed in the sensing region and a sampling wipe may be disposed in the sensing region. The sampling region is configured to collect an analyte. After the analyte is collected on the sampling region, the sampling and sensing device can be folded to make the sampling region in contact with or face the sensing region to transfer the analyte to the sensing region for analysis.

A microfluidic chip orients and isolates components in a sample fluid mixture by two step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

An automatic nucleic acid extraction cartridge and an automatic nucleic acid extraction system including the same are described herein. The cartridge having a housing that includes a sample port, a cell processing chamber, a wash fluid chamber, a filter assembly comprising a filter member, and a diverter valve having a first and a second reversibly sealable output, wherein each of the sample port and the cell processing chamber, the cell processing chamber and the filter assembly, and the wash fluid chamber and the filter assembly are in one-way fluid communication, and the filter assembly is in fluid communication with the diverter valve and (i) a waste conduit when the diverter valve is biased to the first reversibly sealable output and (ii) a pathogen nucleic acid conduit when the diverter valve is biased to the second reversibly sealable output. The present disclosure further describes methods of using the same.