An apparatus for sorting macromolecules includes a first chip including a channel formed in a first side of the first chip and having at least one monolithic sorting structure for sorting macromolecules from the sample fluid. A first set of vias formed in the first chip has openings in a second side of the first chip, the sample fluid being provided to the sorting structure through the first set of vias. A second set of vias formed in the first chip has openings in the second side for receiving macromolecules in the sample fluid greater than or equal to a prescribed dimension sorted by the sorting structure. A third set of vias formed in the first chip has openings in the second side for receiving macromolecules in the sample fluid less than the prescribed dimension. The apparatus includes first and second seals covering the first and second sides, respectively.

The invention relates to a method of forming a sensing device for supporting a plurality of nanopores upon an array of wells. The method involves providing a substrate, said substrate having a surface having an array of electrodes located thereon for connecting to or for configuring upon an electronic circuit. Separately, a well array structure is provided, which has an array of walls defining through-holes for defining wells. The substrate and well array structures are aligning said array of electrodes define, at least in part, a portion of the bases of respective wells at the bottom of the through holes. The resulting sensing device overcomes problems with known sensing devices by employing a substrate and/or well array structure, or hybrid thereof, that employs alternative materials or manufacturing processes.

Disclosed herein, inter alia, are nanoarrays and methods of use thereof.

A fluidic device (1) comprises a flow chamber (2) for accommodating a biological specimen on a carrier portion (3) and at least one flow channel (4a, 4b, 4c, 4d) fluidly connected to the flow chamber (2), the fluidic device (1) having a layered structure comprising a bottom plate (5), a cover plate (6) and an insert (7) in between, the insert (7) comprising the carrier portion (3) and a frame portion surrounding the carrier portion (3), and being elastomeric in order to be able to clamp a biological specimen between an incision in the carrier portion (3).

In a method for creating hydrophilic surfaces or surface regions on one or more silicon surfaces of a substrate, a vapour phase of hydrogen peroxide is generated in a reactor by heating an aqueous hydrogen peroxide solution. The substrate having the silicon surface or surfaces to be treated is exposed to the vapour phase, whereby a hydrophilisation of the silicon surfaces in achieved.

In a method for producing a compound of at least two polymer substrates, two polymer substrates each having a connecting surface are provided. At least one of the polymer substrates is coated with a self-assembling polypeptide, at least in the area of the connecting surface. The two polymer substrates are connected by pressing together the connecting surfaces under pressure and at a temperature corresponding to at least the glass transition temperature of the material of one of the polymer substrates at the connecting surface, wherein a diffusion of polymer chains takes place between the connecting surfaces by the self-assembling polypeptide and a solid connection is formed between the two connecting surfaces. A sealed microfluidic cartridge includes a polymer cartridge and a sealing film connected by such a method.

An apparatus, a system, sensor, and a method for determining dissolved oxygen content in air and aqueous medium are disclosed herein. The dissolved oxygen content may be determined by irradiating the sensor comprising at least a photo-oxidizable compound by a light irradiation source, wherein the irradiation enables the photo-oxidizable compound to change its luminescent properties based upon photo-oxidation thereby enabling the quantification of the dissolved oxygen content in the medium. The dissolved oxygen content may be captured via the impedance response generated by interdigitated conducting electrode patterns included in the sensor. The dissolved oxygen content registered by the interdigitated conducting electrode patterns may be transmitted to a user device via a short range or long-range communication via an electronic circuit embedded within the sensor.

Disclosed is an apparatus and method of forming, including a supporting structure, a sensor on the supporting structure, a pair of columns on the supporting structure at opposite sides of the sensor, the pair of columns having a column height relative to a top surface of the supporting structure, the column height being higher than a height of the active surface of the sensor relative to the top surface of the supporting structure, and a lidding layer on the pair of columns and over the active surface, the lidding layer being supported at opposite ends by the pair of columns. The active surface of the sensor, the lidding layer and the pair of columns form an opening above at least more than about half of the active surface of the sensor, and the supporting structure, the sensor, the lidding layer and the pair of columns together form a flow cell.

A functional material for testing a liquid sample includes a based material in a sheet shape and a channel part provided on a mounting surface of the base material wherein the channel part is composed with water-permeable fibers having permeability, and water-impermeable fibers having impermeability. The water-permeable fibers and the water-impermeable fibers are arranged along the longitudinal direction of the channel part, forming voids wherein the voids are in a mesh structure in which one of the voids connects to another of the voids such that the empty spaces are linked from a base end to a tip end of the channel part. A thickness of the channel part is ranged from 20 μm mm to 5 mm, and a width of the voids is ranged from 10 μm to 200 μm, allowing the liquid sample to move from the base end to the tip end due to capillarity.

A device for imaging sensitive biological samples is provided. The device can include a plastic frame and a glass coverslip, each can be comprised of a biologically compatible material. The device can then be configured such that a biological sample placed therein can only be in contact with biologically compatible materials and, when imaged, provide optimal imaging characteristics by allowing imaging through the glass coverslip.