The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

An electronic driving circuit for a microfluidic device, having a number of synchronized driving stages to generate a respective driving signal for each electrode or group of electrodes of the microfluidic device, the driving signals having a desired amplitude, frequency and phase-shift. Each driving stage has a switching-mode amplifier stage to receive a clock signal and a target signal and to generate, at an output thereof, an output signal defining a respective driving signal. The amplifier stage has: a switching module, coupled to a first internal node and controlled by the clock signal for selectively bringing the first internal node to a control signal; a filter module, coupled between the first internal node and the output, to provide the output signal; and a feedback module.

The disclosure relates to a device for dielectrophoretic capture of particles. The device includes at least one electrical contact and at least one layer. The at least one layer includes a top layer side, a bottom layer side, and a barrier structure. The barrier structure is configured such that a fluid comprising the particles can flow through the barrier structure which is disposed on the top layer side. The barrier structure spaces the top layer side apart from the bottom layer side of at least one of the same layer and a second of the at least one layer.

Embodiments of the present invention relate to a device comprising a platform comprising a layer of a 2-dimensional material. The device further comprises a plurality of electrodes and one or more molecules arranged on the platform. The device is configured to apply control signals to the plurality of electrodes to position the molecules by means of an electric field. Embodiments of the invention further concern a corresponding method for fabricating such a device and a method for positioning molecules by such a device.

A biosensor of the present invention comprises: a first microelectrode and a second microelectrode arranged to intersect in a comb shape on a substrate; and a plurality of receptors fixed in a space between the microelectrodes to specifically react with a target biomaterial. In particular, a micropattern of a conductive material is formed in the space between the microelectrodes. Accordingly, greater electric field intensity can be obtained compared to a biosensor without micropatterns, thereby concentration of the target biomaterial using dielectric electrophoretic forces can be performed more efficiently. In addition, damage to biomolecules can be prevented by lowering the intensity of a voltage for a dielectric electrophoresis phenomenon and the biosensor can be easily commercialized as a health care sensor for diagnosing diseases.

Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.

Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

The present technology relates to improved device and methods of use of insulator-based dielectrophoresis. This device provides a multi-length scale element that provides enhanced resolution and separation. The device provides improved particle streamlines, trapping efficiency, and induces laterally similar environments. Also provided are methods of using the device.

A dielectrophoretic (DEP) sensor includes a graphene electrode adjacent a channel for confining a target particle in a liquid, a surface probe attached to a surface of the graphene electrode, the surface probe having a selective reaction with the target particle, and a voltage source electrically connected to the graphene electrode and configured to apply a voltage to the graphene electrode to cause DEP trapping of the target particle at the graphene electrode.

Devices, systems, and methods for applying a dielectrophoretic force on a particle include: a cell defining at least one channel for confining the particle; and a first electrode and a second electrode electrically isolated from the first electrode, at least one of the first and second electrodes being formed from a two-dimensional (2D) material providing an atomically sharp edge. The first and second electrodes are arranged sufficiently close to one another and sufficiently close to the channel such that application of a sufficient voltage across the first and second electrodes generates an electric field in at least part of the channel, the electric field having an electric field gradient sufficient to apply the dielectrophoretic force on the particle in the channel.