A method for separating a cell culture mixture utilizes a centrifugal separator including a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet for supply of the cell culture mixture to the separation space and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing and arranged so that the separated cell phase is discharged at the rotational axis.

A method for separating a cell culture mixture utilizes a centrifugal separator including a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet for supply of the cell culture mixture to the separation space and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing and arranged so that the separated cell phase is discharged at the rotational axis.

An industrial centrifuge for extracting one or more compounds from biomass includes a centrifuge basket disposed within a cylindrical vessel. The centrifuge basket includes a cylindrical sidewall comprising an upper perforated portion and a lower solid portion and a basket baseplate coupled to the sidewall between the upper perforated portion and the lower solid portion. The centrifuge includes a spindle coupled to the basket baseplate, a spindle bearing assembly, and a contact seal disposed between the spindle bearing assembly and the basket baseplate. The lower solid portion of the sidewall, the spindle, and the basket baseplate define a skirted volume. During operation of the industrial centrifuge, air trapped within the skirted volume provides an air seal between an extraction fluid in the cylindrical vessel and the contact seal, spindle bearing assembly, or both, to prevent extraction fluid from contacting the spindle bearings and causing damage thereto.

An industrial centrifuge for extracting one or more compounds from biomass includes a centrifuge basket disposed within a cylindrical vessel. The centrifuge basket includes a cylindrical sidewall comprising an upper perforated portion and a lower solid portion and a basket baseplate coupled to the sidewall between the upper perforated portion and the lower solid portion. The centrifuge includes a spindle coupled to the basket baseplate, a spindle bearing assembly, and a contact seal disposed between the spindle bearing assembly and the basket baseplate. The lower solid portion of the sidewall, the spindle, and the basket baseplate define a skirted volume. During operation of the industrial centrifuge, air trapped within the skirted volume provides an air seal between an extraction fluid in the cylindrical vessel and the contact seal, spindle bearing assembly, or both, to prevent extraction fluid from contacting the spindle bearings and causing damage thereto.

The invention discloses an analytical sample preparation device and an analytical sample preparation method, which comprises a base (1), an elastic connection body (5), a group of a synchronous unidirectional bearing inner ring (41) and a synchronous unidirectional bearing outer ring (42), a group of an eccentric unidirectional bearing inner ring (61) and an eccentric unidirectional bearing outer ring (62), and a synchronous fixed ring (2) and a motor (31) on the base (1). The eccentric unidirectional bearing inner ring (61) is connected with an eccentric shaft (32) extending from a motor (31). The eccentric unidirectional bearing outer ring (62) is fixed to an eccentric shaft sleeve (9). The eccentric shaft sleeve (9), the eccentric shaft (32), the eccentric unidirectional bearing inner ring (61), and the eccentric unidirectional bearing outer ring (62) have their center of mass disposed on a line extending from the center line of the motor (31). The eccentric unidirectional bearing inner ring (61) and the eccentric unidirectional bearing outer ring (62) can only rotate in direction A, while the synchronous unidirectional bearing outer ring (41) and the synchronous unidirectional bearing outer ring (42) can only rotate in a direction opposite to direction A.

The invention discloses an analytical sample preparation device and an analytical sample preparation method, which comprises a base (1), an elastic connection body (5), a group of a synchronous unidirectional bearing inner ring (41) and a synchronous unidirectional bearing outer ring (42), a group of an eccentric unidirectional bearing inner ring (61) and an eccentric unidirectional bearing outer ring (62), and a synchronous fixed ring (2) and a motor (31) on the base (1). The eccentric unidirectional bearing inner ring (61) is connected with an eccentric shaft (32) extending from a motor (31). The eccentric unidirectional bearing outer ring (62) is fixed to an eccentric shaft sleeve (9). The eccentric shaft sleeve (9), the eccentric shaft (32), the eccentric unidirectional bearing inner ring (61), and the eccentric unidirectional bearing outer ring (62) have their center of mass disposed on a line extending from the center line of the motor (31). The eccentric unidirectional bearing inner ring (61) and the eccentric unidirectional bearing outer ring (62) can only rotate in direction A, while the synchronous unidirectional bearing outer ring (41) and the synchronous unidirectional bearing outer ring (42) can only rotate in a direction opposite to direction A.

An exchangeable separation insert for a centrifugal separator includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged, and first and second stationary portions. A feed inlet supplies a fluid mixture to the separation space. The insert includes a light phase outlet and a heavy phase outlet. The feed inlet is arranged at a first axial end of the rotor casing. One of the light phase outlet and heavy phase outlet is arranged at a second axial end. A first rotatable seal seals and connects the feed inlet and a second rotatable seal seals and connects one of the light phase outlet and heavy phase outlet.

An exchangeable separation insert for a centrifugal separator includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged, and first and second stationary portions. A feed inlet supplies a fluid mixture to the separation space. The insert includes a light phase outlet and a heavy phase outlet. The feed inlet is arranged at a first axial end of the rotor casing. One of the light phase outlet and heavy phase outlet is arranged at a second axial end. A first rotatable seal seals and connects the feed inlet and a second rotatable seal seals and connects one of the light phase outlet and heavy phase outlet.

A base unit and a modular centrifugal separator are disclosed. The base unit includes a stationary frame, a rotatable member, and a drive unit. The rotatable member delimits an inner space being configured for receiving at least one part of an exchangeable separation insert therein. The rotatable member is provided with a first opening at a first axial end configured for a first fluid connection of the exchangeable separation insert to extend through the first opening. The rotatable member includes a second opening at a second axial end configured for a second fluid connection of the exchangeable separation insert to extend through the second opening.

A centrifugal separator for separation of a cell culture mixture includes a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around a vertical axis of rotation. The rotatable assembly includes a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around an axis of rotation. The rotor casing includes a mechanically hermetically sealed inlet and first and second mechanically hermetically sealed liquid outlets. The second mechanically hermetically sealed outlet is arranged at an axial end of the rotor casing opposite and arranged so that a separated cell phase is discharged at the rotational axis. The rotatable assembly includes an exchangeable separation insert and a rotatable member.