A continuous flow centrifuge bowl includes a rotatable outer body, and a top and bottom core that are rotatable with the outer body. The bottom core has a wall extending proximally from a bottom wall. The proximally extending wall is radially outward from at least a portion of the top core and, together with the top core, defines a primary separation region in which initial separation of the whole blood occurs. The bowl may also have a secondary separation region located between the top core and the outer body, and a rotary seal that couples an inlet port and two outlet ports to the outer body. The inlet port may be connected to an inlet tube that extends distally into a whole blood introduction region. Additionally, one of the outlet ports may be connected to an extraction tube that extends into a region below the bottom core.

A method and a device for monitoring the rotational speed of an element are disclosed. The element may be part of a centrifuge, e.g. a motor, drive shaft, or rotor of a laboratory centrifuge. The rotational speed of the element is not determined directly, but rather clock cycles are counted while the element rotates by a predetermined rotational angle. Exceeding a predetermined rotational speed is determined by comparing a number of counted cycles with a predetermined value. The method and device can be implemented in hardware without requiring a microcontroller with corresponding software, thereby eliminating a need to software certification.

A method is provided for controlling separation of solid and liquid phases in a centrifugal basket of a centrifugal. The method includes the steps of monitoring at least associated values of the rotational speed of the centrifugal basket and a purging of the liquid phase from the centrifugal basket, wherein the purging of the liquid phase from the centrifugal basket is monitored using an accelerometer, determining a safe temporal range within which temporal range a wash water can be safely applied to the content of the centrifugal basket, and applying a wash water to the content of the centrifugal basket during at least a portion of the safe temporal range. A computer program product and a centrifugal system are provided for carrying out the method.

Systems and methods are provided for separating blood into two or more components. A blood separation system includes a blood separation device and a fluid flow circuit configured to be mounted to the blood separation device. The blood separation device includes a centrifugal separator and a spinning membrane separator drive unit incorporated into a common case, which allows for fluid separation by two different methods. Depending on the separation procedure to be carried out, the fluid flow circuit paired with the blood separation device may include only one separation chamber configured to be mounted to the centrifugal separator or spinning membrane separator drive unit or two separation chambers, with one being mounted to the centrifugal separator and the other to the spinning membrane separator drive unit. The system may be used to separate and collect any combination of red blood cells, plasma, and platelets.

A centrifuge (10), comprising a rotor (12), a drive shaft (14), on which the rotor (12) is supported, a motor (18), which drives the rotor (12) via the drive shaft (14), a supporting unit (30) having damping elements (36), each of which comprises a spring axis (36a), which supporting unit supports a rotational unit (19), which comprises the motor (18) together with the drive shaft (14) and the rotor (12), a sensor unit (82, 84) for sensing the rotational speed, a distance sensor (80) for determining imbalances of the rotational unit (19), which rotational unit rotates about an axis of rotation (14a), an acceleration sensor (88) for determining imbalances of the rotational unit (19), and a control and evaluation unit (90), which evaluates the data of the sensors (80, 82, 88), wherein the distance sensor (80) senses distance changes in an operative axis (36b). The invention is characterized in that the operative axis (36b) is oriented in relation to the axis of rotation (14a) in such a way that an angle between the operative axis (36b) and the axis of rotation (14a) of less than 90 including 0 results, at least in a projection onto a plane parallel to the operative axis (36b) and through the axis of rotation (14a).

The present invention relates to a method for separating lymphocytes and/or stem cells from whole blood in an automated blood separation system, wherein the quality of the collected lymphocytes and/or stem cells fractions is increased and the cell collection procedure is further automated by use of an optical sensor comprised in a detector device to measure turbidity and colour in the claimed method and in a cell separator, which can be used to perform the claimed method. The method of the invention is particularly useful to collect lymphocytes and/or stem cells fractions from whole blood, wherein the contamination of the collected cell fractions by platelets, red blood cells and granulocytes is reduced.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder, a pump and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A centrifuge rotor having a curved shape is offset on a spinning rotor base and creates contiguous areas of low to high centrifugal force depending on the distances from the axis of the rotor base and a method of separating components in a fluid based upon a difference in density of the components, the method comprising the steps of providing to a rotor as described herein the fluid containing the mixed together components to be separated based upon the difference in density of the mixed together components; continuously flowing the components in the fluid to the rotor through an input tube connected to the input port while the rotor is spinning about a centrifugal axis of rotation; separating the components in the fluid into fractions based upon the difference in density of the mixed together components with the use of centrifugal force when the rotor is spinning; collecting components having i) a first density via a first tube connected to the output port at the first end on the rotor, ii) a second density via a second tube connected to the output port at the second end on the rotor, iii) a third density via a third tube connected to the output port at the junction on the rotor and iv) a fourth density via a fourth tube connected to the output port between the input port and the output port at the first end.

A continuous flow centrifuge bowl includes a rotatable outer body, and a top and bottom core that are rotatable with the outer body. The bottom core has a wall extending proximally from a bottom wall. The proximally extending wall is radially outward from at least a portion of the top core and, together with the top core, defines a primary separation region in which initial separation of the whole blood occurs. The bowl may also have a secondary separation region located between the top core and the outer body, and a rotary seal that couples an inlet port and two outlet ports to the outer body. The inlet port may be connected to an inlet tube that extends distally into a whole blood introduction region. Additionally, one of the outlet ports may be connected to an extraction tube that extends into a region below the bottom core.

A system for concentrating particles in an air stream includes an air channel having a first open end and a second open end. The air channel may be enclosed by a channel wall extending from at least the first open to the second open end. Two or more heater elements may be positioned between the first open end and the second open end. The heater elements may be positioned near a periphery of the air channel and cooperatively configured to force particles in the air stream away from the periphery and towards an interior region of the air channel. Particles in the air stream may be thermophoretically forced towards the interior region of the air channel when the heater elements are heated and thermal gradients emanating from the heater elements are generated.