A processes for solubilization or hydrolysis of a municipal solid waste with an Enzyme composition, comprising: (i) a cellulolytic enzyme composition, and (ii) a protease selected from the group consisting of: (a) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to amino acids 1 to 177 of SEQ ID NO: 1 or a fragment thereof having protease activity; (b) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to SEQ ID NO: 5; (c) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to SEQ ID NO: 32 or a variant thereof; (d) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to SEQ ID NO: 33; and (e) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to amino acids 199 to 564 of SEQ ID NO: 36.

A processes for solubilization or hydrolysis of a municipal solid waste with an Enzyme composition, comprising: (i) a cellulolytic enzyme composition, and (ii) a protease selected from the group consisting of: (a) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to amino acids 1 to 177 of SEQ ID NO: 1 or a fragment thereof having protease activity; (b) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to SEQ ID NO: 5; (c) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to SEQ ID NO: 32 or a variant thereof; (d) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to SEQ ID NO: 33; and (e) a protease having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% identity to amino acids 199 to 564 of SEQ ID NO: 36.

The invention relates to compositions comprising microbial strains and methods for use of the compositions in removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals. More particularly, the invention relates to compositions of isolated Bacillus strains selected from the group consisting of isolated Bacillus strains Bacillus licheniformis MDG-1000 (NRRL No. B-67888), Bacillus licheniformis MDG-1001 (NRRL No. B-67889), Bacillus subtilis/amyloliquefaciens MDG-8001 (NRRL No. B-67890), Bacillus pumilus MDG-1047 (NRRL No. B-67891), Bacillus amyloliquefaciens MDG1607 (NRRL No. B-67666), Bacillus subtilis MDGV18 (NRRL No. B-67665), Bacillus pumilus MDGV17 (NRRL No. B-67664), Bacillus subtilis MDG-1728 (NRRL No. B-67618), Bacillus megaterium MDG-2705 (NRRL No. B-67619), and strains having all of the identifying characteristics of these strains, and combinations thereof, and methods for use of these strains for removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals.

The invention relates to compositions comprising microbial strains and methods for use of the compositions in removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals. More particularly, the invention relates to compositions of isolated Bacillus strains selected from the group consisting of isolated Bacillus strains Bacillus licheniformis MDG-1000 (NRRL No. B-67888), Bacillus licheniformis MDG-1001 (NRRL No. B-67889), Bacillus subtilis/amyloliquefaciens MDG-8001 (NRRL No. B-67890), Bacillus pumilus MDG-1047 (NRRL No. B-67891), Bacillus amyloliquefaciens MDG1607 (NRRL No. B-67666), Bacillus subtilis MDGV18 (NRRL No. B-67665), Bacillus pumilus MDGV17 (NRRL No. B-67664), Bacillus subtilis MDG-1728 (NRRL No. B-67618), Bacillus megaterium MDG-2705 (NRRL No. B-67619), and strains having all of the identifying characteristics of these strains, and combinations thereof, and methods for use of these strains for removing pollutants in waste, water, or soil, such as remediating dye and lignin, reducing contaminants, degrading paper (such as flushable or disposable or non-flushable wipes), reducing odor, reducing chemical oxygen demand (COD), and combinations thereof, in the water, the soil, or the waste or for use in administration to animals in the feed or drinking water of the animals.

A system of waste management and compost generation comprising: a waste collection system wherein waste is collected in separated compostable and non compostable waste streams from at least one source in trackable bins; and wherein the trackable bins contain separated compostable and non compostable waste streams from at least one source; a processing facility which receives and processes the compostable and non compostable waste in the trackable bins; a sorting system for further processing the waste from the trackable bins into a compostable stream and a non compostable stream; a communication and data entry system for collecting the data from the sorting system and reporting the data to at least one source; a composting system which processes the compostable stream into compost material; and a processing system that processes or bundles a high percentage of the non compostable stream into useful products.

A system of waste management and compost generation comprising: a waste collection system wherein waste is collected in separated compostable and non compostable waste streams from at least one source in trackable bins; and wherein the trackable bins contain separated compostable and non compostable waste streams from at least one source; a processing facility which receives and processes the compostable and non compostable waste in the trackable bins; a sorting system for further processing the waste from the trackable bins into a compostable stream and a non compostable stream; a communication and data entry system for collecting the data from the sorting system and reporting the data to at least one source; a composting system which processes the compostable stream into compost material; and a processing system that processes or bundles a high percentage of the non compostable stream into useful products.

Methods and systems for desalination and decontamination for solid waste are disclosed. The method includes decontaminating a volume of contaminated, desalinated drill cuttings by removing at least a portion of contaminants associated with the contaminated, desalinated drill cuttings. The desalinated drill cuttings are drill cuttings that have been desalinated by a desalination unit.

An energy-saving and environment-friendly garbage disposal box capable of dispersing garbage, applicable to various areas, includes a domestic garbage disposal unit, a recyclable garbage disposal unit and a plastic garbage disposal unit. A pedal mashing device is provided to a rear portion of the domestic garbage disposal unit. The garbage disposal box is covered by solar panels and a computer display. An auto electronic weighing plate is provided to top the garbage disposal box. A set of mashing cutters is disposed inside the garbage disposal box to connect spatially with a fermentation area, and an organic fertilizer area is located under the fermentation area. The recyclable garbage disposal unit is furnished with a garbage entrance at an upper portion thereof and three external drawers corresponding to three recycle chambers. The plastic garbage disposal unit covered by solar panels is furnished thereinside a gravity compression iron and a battery set. The garbage disposal box provided by this patent disclosure and featured in flexible garbage dispersing functions can resolve environmental degradation caused by garbage piling and environmental pollution caused by the current garbage disposal means, and can also provide a possibility to maximize the garbage utilization rate.

The present disclosure relates to a non-naturally occurring microorganism that includes a gene encoding a MucK transporter protein, where the microorganism is capable of catabolizing terephthalic acid (TPA). In some embodiments of the present disclosure, the gene encoding the MucK transporter protein may contain at least one mutation, relative to a reference gene encoding a reference MucK transporter protein.

Bacillus subtilis SK01 and its application in plastic degradation. Bacillus subtilis SK01 collected by China Center for Type Culture Collection (CCTCC), CCTCC NO: M2020812 is used to prepare a degradable plastic that can protect Bacillus subtilis SK01 from the damage of high temperature and reduce the difference with polyene plastics in specific gravity. The plastic may be stored for more than 1 year to ensure that the microbial enzymes produced by Bacillus subtilis SK01 can achieve 49.11% of total degradation of organic solids after 153 days in an environment for composting or soil burial, and can be slowly degraded by sunlight (photodegradation) or immersion in water. Under an environment other than that mentioned above, the finished products of polyene plastics added with Bacillus subtilis SK01 are consistent with the non-degradable plastics in shelf life, satisfying the demand of a variety of polyene plastics.