A subsurface soil purification method including: warming an activator liquid, for stimulating decomposer microorganisms that decompose a contaminant in subsurface soil, to a higher temperature than a groundwater temperature, and feeding the activator liquid into the subsurface soil by injecting the activator liquid into an in-ground injection well; warming an activator liquid, for stimulating decomposer microorganisms that decompose a contaminant in subsurface soil, the decomposer microorganisms being infused in the activator liquid, to a higher temperature than a groundwater temperature, and feeding the activator liquid into the subsurface soil by injecting the activator liquid into an in-ground injection well, or warming a purification liquid for decomposing a contaminant in subsurface soil, to a higher temperature than a groundwater temperature, and feeding the purification liquid into the subsurface soil by injecting the purification liquid into an in-ground injection well. The subsurface soil purification method also includes forcing air into the injection well, and feeding the air into the subsurface soil from a position in the injection well that is lower than a position in the injection well for feed-in of the activator liquid or the purification liquid.

The present disclosure provides a method of preparing a carbonaceous material capable of fixing arsenic and an application thereof. Through biomass pretreatment, biomass pyrolysis and arsenite fixation, a biochar activated by potassium carbonate and an arsenic-containing wastewater containing sulfur-containing substances are mixed and deoxidized, and an anaerobic culture is carried out, to fix arsenic by the biochar activated by potassium carbonate. The present disclosure solves the problems that arsenic is released from the soil and groundwater under anaerobic conditions, the ability of conventional passivating agents to fix arsenic under anaerobic conditions is weakened, and the conventional carbonaceous materials not only cannot fix arsenic, but also accelerate the release of anaerobic arsenic.

The present disclosure provides a method of preparing a carbonaceous material capable of fixing arsenic and an application thereof. Through biomass pretreatment, biomass pyrolysis and arsenite fixation, a biochar activated by potassium carbonate and an arsenic-containing wastewater containing sulfur-containing substances are mixed and deoxidized, and an anaerobic culture is carried out, to fix arsenic by the biochar activated by potassium carbonate. The present disclosure solves the problems that arsenic is released from the soil and groundwater under anaerobic conditions, the ability of conventional passivating agents to fix arsenic under anaerobic conditions is weakened, and the conventional carbonaceous materials not only cannot fix arsenic, but also accelerate the release of anaerobic arsenic.

Compositions and uses involving L. monocytogenes PssZ, as well as homologs, variants, and fragments thereof, are described.

A method of remediation of soil and groundwater containing hydrocarbons and halogenated compounds. The method includes introducing a remediation composition into the soil that includes: (a) a first bioremediation material including a first blend of organisms capable of degrading the hydrocarbons; (b) a second bioremediation material including a second blend of organisms differing from the first blend of organisms that is chosen for degrading the halogenated compounds; (c) an organic compound such as a complex carbohydrate (e.g., food grade starch); and (d) a third blend of organisms degrading the organic compound. The degrading of the organic compound breaks the complex carbohydrate into smaller molecules that are utilized by the microorganisms of at least one of the first and second bioremediation materials during the degrading of the hydrocarbons and the halogenated compounds. The first bioremediation composition typically includes activated carbon capable of adsorbing the hydrocarbons and the halogenated compounds.

A method of remediation of soil and groundwater containing hydrocarbons and halogenated compounds. The method includes introducing a remediation composition into the soil that includes: (a) a first bioremediation material including a first blend of organisms capable of degrading the hydrocarbons; (b) a second bioremediation material including a second blend of organisms differing from the first blend of organisms that is chosen for degrading the halogenated compounds; (c) an organic compound such as a complex carbohydrate (e.g., food grade starch); and (d) a third blend of organisms degrading the organic compound. The degrading of the organic compound breaks the complex carbohydrate into smaller molecules that are utilized by the microorganisms of at least one of the first and second bioremediation materials during the degrading of the hydrocarbons and the halogenated compounds. The first bioremediation composition typically includes activated carbon capable of adsorbing the hydrocarbons and the halogenated compounds.

A method of treating or remediating contaminated material, such as water or soil, comprises contacting such material with asphaltenes. The asphaltenes are preferably produced as a by-product of petroleum refining and, in particular, a by-product of vacuum residua. An adsorbent material comprising such asphaltenes is also provided.

A method of treating or remediating contaminated material, such as water or soil, comprises contacting such material with asphaltenes. The asphaltenes are preferably produced as a by-product of petroleum refining and, in particular, a by-product of vacuum residua. An adsorbent material comprising such asphaltenes is also provided.

Methods for bioremediation of environmental media contaminated with at least one perchlorate compound. A Pseudomonas consortium of P. putida strain B, P. putida strain E, and P. fluorescens strain G was provided to contaminated water, soil, etc. under conditions to result in bioremediated water, soil, etc. In embodiments, the method is used ex-situ, e.g., in a reactor vessel, or is used in-situ.

Methods for bioremediation of environmental media contaminated with at least one perchlorate compound. A Pseudomonas consortium of P. putida strain B, P. putida strain E, and P. fluorescens strain G was provided to contaminated water, soil, etc. under conditions to result in bioremediated water, soil, etc. In embodiments, the method is used ex-situ, e.g., in a reactor vessel, or is used in-situ.