A string of reactant chambers configured for inserting a reactant into each reactant chamber, in the string of reactant chambers, while maintaining the reactant chamber being filled in the string of reactant chambers is provided. The string of reactant chambers has a first reactant chamber, a second reactant chamber, and a system configured to maintain the reactant chamber being filled in the string of reactant chambers. The system has a first removable end cap on each reactant chamber and a coupler on each reactant chamber configured for the removal of the first removable end cap and maintaining the reactant chamber in the string of reactant chambers. A process for charging a reactant chamber is also presently disclosed.

A string of reactant chambers configured for inserting a reactant into each reactant chamber, in the string of reactant chambers, while maintaining the reactant chamber being filled in the string of reactant chambers is provided. The string of reactant chambers has a first reactant chamber, a second reactant chamber, and a system configured to maintain the reactant chamber being filled in the string of reactant chambers. The system has a first removable end cap on each reactant chamber and a coupler on each reactant chamber configured for the removal of the first removable end cap and maintaining the reactant chamber in the string of reactant chambers. A process for charging a reactant chamber is also presently disclosed.

A composition remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

A composition remediation of soil and groundwater containing halogenated compounds. The remediation composition includes an elemental iron-based composition, which may include activated carbon capable of absorbing the halogenated compounds with numerous pores impregnated with elemental iron. The remediation composition further includes a first bioremediation material including a blend of one-to-many organisms capable of degrading the halogenated compounds. The remediation composition includes an organic compound or polymeric substance and a second bioremediation material including a blend of one-to-many organisms capable of degrading the organic compound or polymeric substance over time (e.g., 20 to 365 or more days to provide a time release substrate-creating material or platform) into smaller molecules or compounds used by the organisms in the first bioremediation material while degrading the halogenated compounds. The organic compound may be a complex carbohydrate such as food grade starch, chitin, or other complex carbohydrate such as one with low water solubility.

A process in which a waste stream containing microbes and organic constituents is passed through a process environment comprising a solid media, microbes, and higher animals, such that some of the microbes and/or organic constituents within the waste stream are removed from the waste stream and some of the removed microbes are destroyed or consumed by the higher animals. The process environment may include an irrigated environment, a submerged environment, or a combined environment.

A process in which a waste stream containing microbes and organic constituents is passed through a process environment comprising a solid media, microbes, and higher animals, such that some of the microbes and/or organic constituents within the waste stream are removed from the waste stream and some of the removed microbes are destroyed or consumed by the higher animals. The process environment may include an irrigated environment, a submerged environment, or a combined environment.

A method for removing pollutants by using a mixed microbial solution of beneficial microorganisms to enhance electrokinetic force. It includes a mixed microbial solution; an anode tank and a cathode tank are connected to an external power supply. A soil tank contains contaminant soil and the soil tank is positioned between the anode tank and the cathode tank which are parallel to each other. A soil-retaining net is disposed over the soil. The external direct current is used to allow the mixed microbial solution to transfer in the soil tank.

The present disclosure relates to a device and method for remedying a heavy-metal-polluted soil with a microorganism, and relates to a device for remedying a metal-polluted soil and a remediation method thereof. The present disclosure solves the problem that a remediation microorganism cannot be extracted from the soil. The device comprises a main plate and a plurality of microorganism-extracting chambers, wherein the main plate is connected to the microorganism-extracting chambers; the microorganism-extracting chamber are filled with a filler; a tail end of the microorganism-extracting chambers is spiky, and openings are separately provided on a side wall of the microorganism-extracting chambers. The method comprises: I, adding a nutrient medium to the soil, and then inoculating a remediation microorganism; and II, inserting the above device into the soil for 6˜8 days, and then taking it out. The method can remedy heavy-metal-polluted soil in a short time with a high removal rate of the heavy metal.

The present disclosure relates to a device and method for remedying a heavy-metal-polluted soil with a microorganism, and relates to a device for remedying a metal-polluted soil and a remediation method thereof. The present disclosure solves the problem that a remediation microorganism cannot be extracted from the soil. The device comprises a main plate and a plurality of microorganism-extracting chambers, wherein the main plate is connected to the microorganism-extracting chambers; the microorganism-extracting chamber are filled with a filler; a tail end of the microorganism-extracting chambers is spiky, and openings are separately provided on a side wall of the microorganism-extracting chambers. The method comprises: I, adding a nutrient medium to the soil, and then inoculating a remediation microorganism; and II, inserting the above device into the soil for 6˜8 days, and then taking it out. The method can remedy heavy-metal-polluted soil in a short time with a high removal rate of the heavy metal.

A phage and use thereof in soil remediation are disclosed. The phage φYSZPK has been deposited at the China Center for Type Culture Collection on Aug. 1, 2018 under Accession No. CCTCC M 2018516, and its taxonomic designation is Pseudomonas aeruginosa and Klebsiella phage φYSZPK. Biochar and the screened phage are combined and returned into contaminated soil to synergistically control and deeply track and inactivate transmission and spread of antibiotic resistance pathogenic bacteria and resistance genes in a soil-vegetable system. The combination of the biochar and the phage φYSZPK not only clearly improves the functional stability of microbial community in the soil-vegetable system, but also significantly alleviates the dissemination of the antibiotic resistance pathogenic bacteria in the soil-vegetable system to prevent secondary pollution, thereby providing a new solution for biological remediation and control of farmland soil contaminated by antibiotic resistance pathogenic bacteria in China.