The method of manufacturing a mold includes the steps of: forming a silicone resin film on a surface of a model having a protrusion pattern; pressing a side of a base plate with which a separation sheet is in close contacts on a gap adjustment mechanism and the silicone resin film; heating and curing the silicone resin film to form the mold; releasing the base plate; and releasing the separation sheet from the mold, after releasing the mold from the model, wherein the gap adjustment mechanism has a height at which a tip of the protrusion pattern sticks into the separation sheet when the base plate is pressed on the gap adjustment mechanism. The method of manufacturing a pattern sheet uses the mold.

A unitary elastic mold and cutter combination configured to mold and cut moldable material is disclosed herein. The mold and cutter combination comprises an elastic mold cavity having an elastic bottom wall with an embossed inner surface and an elastic sidewall extending from and unitary with a perimeter of the bottom wall. An elastic cutting blade extends from and is unitary with the sidewall of the mold cavity and is configured to cut moldable material and maintain a substantially consistent perimeter upon cutting. The cutting blade comprises a cutting edge disposed between an inner cutting blade surface and an outer cutting blade surface. The mold and cutter combination may have an elasticity sufficient to release molded material therefrom by hand stretching, wherein the cutting edge, inner cutting blade surface, and the inner surface of the sidewall of the mold cavity are stretched by an amount sufficient to release the molded material.

A method includes providing a mold defining a mold cavity for receiving material to be molded into a molded part, positioning an insert at least partially in the mold such that a portion of the insert defines a portion of the mold cavity, and injecting the material into the mold cavity to substantially fill the mold cavity to form the molded part. The molded par can then be removed from the mold and the insert can be removed from the molded part. The mold can include silicone and the insert can include polyoxymethylene.

A method of forming a three-dimensional object, is carried out by (a) providing a carrier and a build plate, the build plate comprising a semipermeable member, the semipermeable member comprising a build surface with the build surface and the carrier defining a build region therebetween, and with the build surface in fluid communication by way of the semipermeable member with a source of polymerization inhibitor; (b) filling the build region with a polymerizable liquid, the polymerizable liquid contacting the build surface, (c) irradiating the build region through the build plate to produce a solid polymerized region in the build region, while forming or maintaining a liquid film release layer comprised of the polymerizable liquid formed between the solid polymerized region and the build surface, wherein the polymerization of which liquid film is inhibited by the polymerization inhibitor; and (d) advancing the carrier with the polymerized region adhered thereto away from the build surface on the build plate to create a subsequent build region between the polymerized region and the build surface while concurrently filling the subsequent build region with polymerizable liquid as in step (b). Apparatus for carrying out the method is also described.

A sterile enclosure intended for a printing of a biological three-dimensional structure. This enclosure includes: a support, a covering cap including a rigid part and a flexible part extending in the prolongation of the rigid part, the covering cap being sealed to the support to delimit an interior chamber, at least one portion of the rigid part being a septum adapted to be punctured by a printing needle for printing a biological three-dimensional structure in the interior chamber. Also, a biological printing device and a method of printing.