An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.

Provided herein is technology relating to detecting one or more analytes in a sample and particularly, but not exclusively, to devices, methods, systems, and kits for detecting one or more drugs-of-abuse analytes in a biological sample.

A method includes assembling a sensor subassembly that includes a sensor, a sensor mount, a collar, a sharp, and a sensor cap. The method includes loading a sensor in a sensor mount; dispensing adhesive into a mount channel of the sensor mount; clamping a collar to the sensor mount; and curing the adhesive to fix the collar to the sensor mount. The method can also include inserting a sharp into the sensor mount over the sensor an attaching a sensor cap to the sensor and sensor sharp to provide a sealed sensor subassembly. Methods of assembling an on-body sensor puck assembly and an applicator assembly, and a sensor including a tail, a flag, and a neck that interconnects the tail and the flag and methods of configuring a sensor are also disclosed.

Method of performing a droplet-based assay. The method may include obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer, performing a reaction in the droplets while packed closely together in the monolayer; and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.

A method of manufacturing an annular insert molded article that suppresses contamination of a metal mold and generation of foreign matter without increase in material costs for an adhesive or contamination of a transfer jig, and has a high adhesion strength so that, when the annular insert molded article is attached to a rotating body, the adhesive does not peel off from the insert or remain as foreign matter in the rotating body. An annular insert molded article 1 is manufactured by injection molding in a state where a thermoset resin adhesive A is applied to a joining surface of an annular insert 2 attachable to a rotating body to an annular plastic 3 and then the annular insert 2 and the annular plastic 3 are placed in a metal mold.

A sample cell (10) for a respired gas sensor has a single-piece injection molded main body (40) defining a gas flow path including an optical sampling bore (42), a gas inlet lumen (50) connected with the inlet end (44) of the optical sampling bore, and a gas outlet lumen (52) connected with the outlet end (46) of the optical sampling bore. The gas flow path includes at least two curved walls (100, 102, 104, 106). The sample cell may be manufactured by assembling mold pins (120, 122, 124, 126, 128) for defining the gas flow path wherein at least two mold pins (122, 124) have curved surfaces for defining the at least two curved walls of the gas flow path, and injection molding the single piece injection molded main body including removing the mold pins after defining the gas flow path including the at least two curved walls.

System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

A method for manufacturing an enclosure for a utility meter is provided, wherein at least two of a main cover part, an exterior terminal cover part, and an interior terminal cover part are at least partly formed jointly as a cover assembly in an injection moulding device. Further provided is a utility meter with an enclosure manufactured by the method.

The disclosed technology includes devices and methods for detecting rare cells in whole blood samples using a microfluidic device. Such devices include a housing with a microfluidic chamber having first and second microfluidic layers, each microfluidic layer having an array of microscale structure. Other disclosed devices also include a housing including a chemically functionalized hydrogel matrix and a pump connected to the housing. Disclosed methods include constructing, with an additive manufacturing device, a microfluidic device having a microfluidic chamber, removing, by a thermal release process, at least some of the sacrificial support material deposited by the additive manufacturing device, and chemically functionalizing at least a portion of the microfluidic chamber. Other disclosed methods include chemically functionalizing a hydrogel matrix and connecting the chemically functionalized hydrogel matrix to a pump.

A vehicle trim assembly includes a first trim component having an interior facing surface and an exterior facing surface, the first trim component including an extended portion, a second trim component including an edge defining a groove configured to engage with the extended portion of the first trim component, and a single chip sensor overmolded with the second trim component such that the single chip sensor is integrated into the second trim component. The single chip sensor is formed with the second trim component in a multi-shot injection molding process.