The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.

The present application includes a symbiotic reverse osmosis train system for maximizing desalinated water recovery, meanwhile yielding high salinity brine suitable for osmotic power generation or commercial salt production. The trains comprise a series of cells operating in an interrelated sequential pattern within a salinity field. Each cell forms a closed hydraulic brine loop having pumping means, power recovery means and shared semipermeable membranes between adjacent cells. Used are a semipermeable Flat Sheet or Hollow Fiber Membrane in desalination and osmotic power generation of brackish, seawater and brines of 15% salinity or more. Charging each cell in the train of cells with a formulated brine having a specified ionizable inorganic salt concentration and type, without permitting mixing of the given brines among adjacent cells. Allowing the train to achieve water recovery exceeding 85% with concentrated rejected brine of 28-30% salt content.

A membrane assembly for separating a feed liquid into a permeate and a retentate includes a semipermeable membrane and conductive members for applying a voltage effective for charging a semipermeable surface of the membrane, thereby reducing or preventing fouling or scaling of the membrane. The conductive members may be positioned adjacent to the semipermeable membrane, and may be configured as feed spacers or permeate spacers. Alternatively or additionally, the membrane may be electrically conductive. Power from an external source may be supplied to one or more of the conductive members, or also the membrane if conductive, which may be done wirelessly. One or more membrane assemblies may be provided in a container. One or more membrane assemblies may be provided in a stacked configuration, or wrapped around a tube in a spiral configuration.

A filter system (100) for biopharmaceutical processes includes: at least one filter unit (10); and at least one distributor plate (200), against which the filter unit rests. A tubeless distributor unit (202) is arranged within the distributor plate and is fluidically connected to the filter unit. The distributor unit guides the fluid to be filtered to the filter unit and/or receives and discharges the filtered fluid from the filter unit. The distributor unit includes at least one active control element (232; 238), with which a fluid flow (14) through the distributor unit) and the filter unit is controlled in an open loop flow or a closed loop flow. The fluid connection between the filter unit and the distributor plate is tubeless. Also provided are a distributor plate (200) for a filter system (100) for biopharmaceutical processes and a method for producing a filter system (100) for biopharmaceutical processes.

Stack assembly comprising a hollow external housing having a central axis, the external housing extending from a first end to a second end and enclosing a housing space, and a membrane stack comprising a plurality of membranes, wherein the membrane slack is positionable inside the external housing and a number of side plates extending substantially parallel to the central axis, wherein each side plate of the number of side plates is associated with a side of the membrane stack and extending along the associated side and a number of sealing connectors that extend substantially parallel to the central axis and adjacent with an inner surface of the external housing, wherein each sealing connector of the number of sealing connectors is configured to connect two side plates to each other, wherein the sealing connectors and the side plates cooperate to form an enclosing structure, and wherein, in use of the stack assembly, the enclosing structure encloses the membrane stack. The invention also relates to a method for assembling a stack assembly and a method for generating energy or performing an electrodialysis process.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

A disc tube reverse osmosis module is provided. In various embodiments, deflector disc assemblies and associated membranes are sequentially alternately disposed from top to bottom. The upper flange is in sealing connection with the central pull rod, the upper lock nut is in threaded connection with an upper end of the central pull rod and located above the upper flange, the yielding water collecting pipe sleeves the central pull rod and is in sealing connection with a lower side of the lower flange, the lower lock nut is in threaded connection with a lower end of the central pull rod, an end of the yielding water receiving pipe is fixedly connected to the yielding water collecting pipe, an inner surface of the shell is respectively in sealing connection with the upper flange and the lower flange, and the water inlet receiving pipe and the concentrated liquid receiving pipe are fixed to the lower flange.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

An implantable or wearable kidney enclosure that is cylindrical, ovoid, or otherwise non-angular e.g., not rectangular or cuboid), having a circular or oval hemofilter that provides a blood flow pattern from an internal, central artery source radially outwards. Due to the efficient flow of the circular filter design, the enclosure can be made in a cylindrical low profile shape, resulting in a compact enclosure highly suitable for implantable and wearable dialysis applications.

A filtration cassette including a multilaminate array of sheets including filter sheets alternating with permeate sheet members and retentate sheet members, and a cross-flow filter device comprising a multiplicity of stacked filtration cassettes of such type. The improvements associated with the filtration cassettes described herein include, but are not limited to, at least one of: reinforced inlet(s) providing for longevity and improved cleanability of the cassettes; spacer permeate screens to limit throughput restriction; stainless steel or otherwise stiffened permeate sheets to prevent movement and increase flux; and stainless steel permeate sheets that can be used with ultrasonic transmission to minimize fouling of the filter sheets and extend cleaning cycles. Advantageously, the filtration cassettes are more resistant to higher temperatures than the filtration cassettes of the prior art.