The present invention an apparatus and method of injecting a liquid borne sample into a field flow fractionator and a method of forming a top plate and spacer. In an embodiment, the field flow fractionation unit includes a top plate including a sample injection inlet port, a sample injection outlet port, and a spacer including a separation channel cavity defining at least a portion of the separation channel, a sample injection inlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection inlet port, a sample injection outlet cavity configured to be in fluid contact with the separation channel and located substantially beneath the sample injection outlet port, such that the injection inlet and outlet paths are configured to define an injection channel that is essentially perpendicular to the length of the separation channel spanning the width of the separation channel cavity.

The present invention relates to a filtration unit with a filtration membrane, nutritive cardboard disc and, if necessary, a support structure, wherein the nutritive cardboard disc and/or the support structure comprises a solid, water-soluble nutrient medium and a water-soluble and/or water-swellable polymer, a method for producing the filtration unit, a method for detecting microorganisms in a fluid, wherein the filtration unit is used, and the use of the filtration unit for detecting microorganisms in a fluid.

A filter holder for liposome extrusion includes a housing having an inlet configured to receive a material to be extruded and an outlet, and a filter support member disposed within the housing between the inlet and the outlet. The filter support member includes an upstream side having a filter support surface configured to support a membrane filter assembly, a downstream side opposite the upstream side, and a plurality of passages extending through the filter support member from the filter support surface to the downstream side. The filter holder also includes an outlet cavity in fluid communication with the outlet, and the filter holder is configured such that the material to be extruded flows through the membrane filter assembly and into the outlet cavity via the plurality of passages before being discharged through the outlet.

A filtration assembly for filtering nanoparticles includes a filter having pores that can retain nanoparticles likely to be found in an air flow passing through the filter and a filter support including two parts. A lower base-forming part of the filter support includes a peripheral bearing surface on which the filter can rest. An upper ring-shaped part of the filter support is designed to be mounted around the bearing surface of the base. By mounting the ring around the bearing surface of the base it is possible to tension the filter radially to the direction of mounting. The mounting clearance between the ring and the bearing surface of the base is dimensioned such as to maintain the filter resting on the bearing surface under mechanical stress by means of pinching, in a direction radial to the mounting direction.

A method for exosome separation and extraction by stacked centrifugal filtration. It is used in molecular biology and clinical examination and comprises an exosome separation and extraction kit consisting of the stacked centrifugal filtration device, an incubation buffer and a protease K. The sample to be tested is incubated at room temperature using the incubation buffer and an appropriate amount of protease K, followed by centrifugation in a centrifuge matching the stacked centrifugal filtration device. After mixing thoroughly, the retained liquid in the ultrafiltration tube is collected to obtain the exosomes. The method needs no large experimental equipments except for a centrifuge, which has a low cost and which is convenient and fast, with short operation time and the possibility of carrying out parallel operations with a large number of samples. The high purity exosomes obtained by the method can meet the demand of large-scale clinical applications.

A cell-capturing device includes: one or more introduction channels for introducing test liquid or treatment liquid; a discharge channel for discharging the test liquid or the treatment liquid to the outside; a filter having a plurality of through-holes, and being disposed in a channel so that the test liquid or the treatment liquid passes through the through-holes; an introduction region formed between the filter and the introduction channel in a channel; a discharge region formed between the filter and the discharge channel; and a housing part accommodating at least a part of the introduction channel, the introduction region, and the discharge region therein, wherein the cell-capturing device further includes a pre-treatment part formed at a position apart from a connection part with the introduction region on at least one of the introduction channels, and formed by a spatial region having a diameter larger than that of the introduction channel.

A filtration system, a filtration assembly, and a method for using the filtration assembly to determine the presence or absence of microorganisms in a fluid filtered by the filtration assembly, are disclosed.

The present invention relates to a method for impregnating a filter having pores suitable for retaining particles within them that may be present in a flow of air suitable for passing through the filter, according to which the filter made up of a polymer membrane is impregnated with one or more organometallic salts by applying a treatment using supercritical CO.sub.2, the metal M of each salt being chosen from among the group of rare earths, yttrium, scandium, chromium, or a combination thereof. The invention also relates to the obtained filter and an associated method for the collection and quantitative analysis of nanoparticles.

A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.

A method of vacuum membrane filtration including placing a membrane filter between a filtration base and a pouring funnel, an upper side of the filtration base having a membrane bearing area with a bearing structure and a supporting contour surrounding the bearing structure and the supporting contour having at least one notch in flow connection with a bottom side of the membrane bearing area, detachably mounting the pouring funnel on the filtration base thereby clamping the membrane filter between the filtration base and the pouring funnel, applying suction to the filtration base such that the membrane filter is pulled against the bearing structure and comes into contact with the supporting contour, and dismounting the pouring funnel from the filtration base while still applying the suction, causing an outer rim of the membrane filter to bulge upward from the supporting contour and uncover the at least one notch.