Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.

An assembly includes a housing with opposite first and second layers. The first and second layers are spaced apart to define a confined interior space. A semi-permeable membrane is attached to the first layer, the semi-permeable membrane covering a porous area portion of the first layer. An outlet port and an inlet port are in fluid communication with the interior space. The assembly includes a first circulator for circulating a first fluid between the outlet port and the inlet port, and a second circulator for circulating a second fluid along an exterior surface of the semi-permeable membrane. The second circulator includes a fluid duct attached to or integrated within the housing. The fluid duct is isolated from the interior space and is porous to provide fluid access to an exterior surface of the semi-permeable membrane. The semi-permeable membrane forms a barrier allowing exchange of compounds across the membrane.

An assembly includes a housing with opposite first and second layers. The first and second layers are spaced apart to define a confined interior space. A semi-permeable membrane is attached to the first layer, the semi-permeable membrane covering a porous area portion of the first layer. An outlet port and an inlet port are in fluid communication with the interior space. The assembly includes a first circulator for circulating a first fluid between the outlet port and the inlet port, and a second circulator for circulating a second fluid along an exterior surface of the semi-permeable membrane. The second circulator includes a fluid duct attached to or integrated within the housing. The fluid duct is isolated from the interior space and is porous to provide fluid access to an exterior surface of the semi-permeable membrane. The semi-permeable membrane forms a barrier allowing exchange of compounds across the membrane.

A system for growing and harvesting algae biomass includes a photo-bioreactor suitable for algae growth in water and a filter unit in fluid communication with the photo-bioreactor. An algae slurry, when at least partially contained within the photo-bioreactor, generates hydrostatic fluid pressure that exclusively drives the algae slurry to the filter unit and discharges a permeate.

A filtration module for filtering a process medium in a bioprocess including a process flow path for a process medium, a filter membrane coupled to the process flow path, and a sampling membrane coupled both to the process flow path and to a sampling flow path for extracting a sample from the process medium. The sampling flow path guides the extracted sample to a sampling outlet of the filtration module or to an analyzer integrated into the filtration module. A filtration assembly including the filtration module and an analyzer coupled to the sampling outlet of the filtration module. A method of sampling during a bioprocess including providing such a filtration module, urging a process medium through the process flow path, filtering the process medium by using the filter membrane, extracting a sample from the process medium by using the sampling membrane, and guiding the extracted sample to a sampling outlet.

Disclosed are a polysaccharide-peptide composite and a method of preparing the same. The polysaccharide-peptide composite is prepared from a bitter melon peptide (BMP) powder, gardenia fruit oil, a soybean polypeptide powder, an oat dietary fiber powder, a konjac powder, a corn silk, a mulberry leaf extract, a Poria cocos extract, a hawthorn extract, nutritional yeast and a pancreatin. The BMP powder is prepared by temperature-controlled hydrolysis, staged enzymatic hydrolysis and multiple filtrations. The gardenia fruit oil is prepared by staged enzymatic hydrolysis, multi-step centrifugation, filtration and stratification.

A process for the efficient transfer of molecules between phases employing mesoporous poly (aryl ether ketone) hollow fiber membranes is provided. The method addresses the controlled transfer of reactants into and removal of reaction products from a reaction media and the removal and separation of target molecules from process streams by membrane-assisted liquid-liquid extraction. A number of possible modes of liquid-liquid extraction are possible according to the invention by utilizing porous poly (aryl ether ketone) hollow fiber membranes of Janus-like structure that exhibit a combination of hydrophilic and hydrophobic surface characteristics. The method of the present invention can address the continuous manufacture of chemicals in membrane reactors and is useful for a broad range of separation applications, including separation and recovery of active pharmaceutical ingredients.

The present disclosure relates to hollow fiber tangential flow filter units, including hollow fiber tangential flow depth filter units, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.

A process for recovery and purification of HMOs comprising: (a) providing an HMO-containing fermentation broth comprising biomass; (b) separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream; (c) purifying the separated HMO-containing stream; (d) concentrating the separated HMO-containing stream; and (e) drying the product of steps (a)-(d) by an indirect drying method thereby forming a purified HMO, wherein steps (c)-(d) can be performed in any order.

The oxygenator of organic fluids comprises: a container body having a longitudinal axis; a first inlet opening for the oxygen and a second outlet opening for an exhaust gas obtained in the container body; a third inlet opening for an organic fluid to be oxygenated and a fourth outlet opening for oxygenated organic fluid obtained in the container body; an oxygenation chamber of the fluid to be oxygenated that is defined inside the container body; a distribution pre-chamber of the fluid to be oxygenated fitted between the third inlet opening and the oxygenation chamber; a mass of capillary fibers that are impermeable to liquids and porous to gasses, designed to be lapped by the organic fluid and arranged inside the oxygenation chamber according with a common parallel direction; dynamic distribution means supported in the distribution pre-chamber by support means.