[Object] To provide a method for isolating and purifying a minute useful substance. [Solution] Disclosed is a method for isolating and purifying a minute useful substance. The method includes filtering a liquid containing a minute useful substance through a hollow fiber membrane. The hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100000 to 1000000. The filtering includes a first filtration process of press-fitting the liquid containing the minute useful substance from a first opening on one end side of the hollow fiber membrane and filtering the liquid to separate the liquid into a permeate and a first concentrate, and a second filtration process of press-fitting the first concentrate from a second opening on the other end side of the hollow fiber membrane and filtering the first concentrate to separate the first concentrate into a permeate and a second concentrate. A concentrate is produced in which a concentration of the minute useful substance is increased by filtration in which the first filtration process and the second filtration process are alternately performed a plurality of times at a membrane surface velocity of 0.3 m/sec to 2 m/sec.

Hollow fiber cartridges and components and methods of their construction.

A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the spinning membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib or ridge associated with the spinning membrane that decreases the gap between the spinning membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously-collected whole blood into selected blood components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed.

A filtration device includes features for robust filtering capability. In particular, the filtration device includes a filter medium having mixed matrix membranes that include hollow fiber membranes embedded with adsorbents such that the filter medium provides both mechanical and adsorption capability.

A water filtration system utilizes a housing configured to utilize various types of filter media and having a press-sealed closing system. The closing system includes a head portion defining a head opening, a lock member, and a lid member. The lock member releasably engages the head portion such that an abutment section of the lock member projects into the head opening. The lid member is received by the head portion to cover the head opening, and has an outward-facing press section configured to abut an inward facing portion of the abutment section of the lock member when the lid member is covering the head opening. Pressure generated within the housing during water filtration presses the lid member in an outward direction and causes the lid member press section to press against the abutment section of the lock member to substantially prevent disengagement of the lock member from the head portion.

A hollow fiber membrane module includes a housing having an inlet formed one end thereof and an outlet formed on the other end thereof, in which hot and humid humidifying fluid flows in through the inlet and the humidifying fluid after humidifying a cool and dry fluid flowing out of the housing through the outlet; at least one hollow fiber membrane bundle which is inserted into the housing in the longitudinal direction; a partitioning part for supporting the hollow fiber membrane bundle and partitioning an inflow space, in which the humidifying fluid flowing into the housing momentarily remains, and an outflow space, in which the humidifying fluid momentarily remains before being discharged through the outlet; and a potting part for potting both ends of the hollow fiber membrane bundle to the housing.

A mitochondria extraction apparatus provided in the present invention includes a container used for holding a mixture fluid and a diafiltration assembly disposed in the container and used for isolating mitochondria. A basement of the mitochondria extraction apparatus is disposed in the container. During a circular motion of the mitochondria extraction apparatus, a centrifugal force in the mitochondria extraction apparatus acts on the mixture fluid in a hole-shaped microfluidic channel included in the diafiltration assembly, so as to provide a pushing force for the mixture fluid, enabling the mixture fluid to rapidly flow in the microfluidic channel for diafiltration.

A hollow fiber module (1, 1) is made by inserting a bundle of hollow fibers (3) into a housing (2, 2) that has an end closed by a cap (17). A fluid first component (27) that can assume a solid state is introduced into a space adjacent the cap (17) and forms a spacer into which ends of the hollow fibers (6, 7) project. A fluid curable second component (4) is introduced before the first component (27) via a second inflow (28) and is cured to form a sealing layer (9) that embeds the bundle of hollow fibers (3) and seals it with respect to the adjacent housing wall (29, 29). The cap (17) is removed from the module housing (2, 2) along with the first component (27) and ends of the hollow fibers (6) embedded in the first component (27).

Systems and methods using: a membrane unit to treat fluids recovered from an oil and gas well are provided. The membrane unit may include a membrane having a porous substrate at. least partially coated with graphene oxide, making the membrane hydrophilic. The membrane separates water from other components within a fluid stream. The membrane unit may include an inlet to receive a fluid stream into the membrane unit. The fluid stream may be pretreated prior to reaching the membrane unit The membrane unit may also include a first outlet in fluid communication with one side of the membrane and a second outlet in fluid communication with the opposite side of the membrane.

A purifier for removing metal, such as chromium, from an organic solvent is disclosed. The purifier comprises a housing having a fluid inlet and a fluid outlet in fluid communication with the fluid inlet; a volume of ion-exchange resin disposed within the housing downstream of the fluid inlet; and a filtration member downstream of the volume of ion-exchange resin, the filtration member comprising at least one microporous membrane having a substantially neutral surface and a microporous polytetrafluoroethylene membrane downstream of the at least one microporous membrane. A method for removing metal, including chromium, from an organic solvent using a purifier of the invention is also disclosed.