The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

A deep sludge dewatering method using electroosmosis with filter bags, including (1) placing a filter bag on a slope on which a cathode electrode is arranged; (2) injecting sludge into the filter bag, and after the filter bag is filled with the sludge, closing an inlet of the filter bag; and (3) laying an anode electrode on the filter bag filled with the sludge, and connecting the cathode electrode and the anode electrode to a DC power supply via an electric wire, and carrying out energization for electroosmosis so that water flows down the slope. The present invention can be used for recycling of the sludge produced in underground and tunnel excavation projects, and has the advantages of large processing capacity, simple process, good treatment effect and available resource recycling.

A fixed tube of a nucleic acid extraction component and a nucleic acid extraction component. The nucleic acid extraction component includes a membrane column, which is fitted over the fixed tube. The fixed tube has a tube body, a tube opening, and a bottom. The tube body extends along a first direction. The end of the tube opening distal from the tube body has a protrusion which protrudes along a second direction, the first direction being vertical to the second direction. The bottom and the tube opening are respectively connected to two opposite sides of the tube body. The bottom has a shoulder and the shoulder of the bottom is connected to the membrane column.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

A membrane contactor includes: a cap has an internally beveled surface and a cap port; a cup body has an externally beveled surface in sealing engagement with the internally beveled surface, a side port on a side of the cup body and an end port located on an end of the cup body; and a membrane cartridge is located within the cup body, is sealed to an open end of the cup body, and is in sealed fluid communication with the end port. A method of making a membrane contactor includes the steps of: sealingly mating a perforated center of a membrane contactor with the end port of a cup body; sealingly joining an end of the membrane cartridge adjacent an open end of the cup body; and sealingly joining a beveled surface of the cap to a beveled external surface of the cup body.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

One aspect of the present disclosure is a membrane for water treatment. The membrane preferably includes at least one membrane sub-unit, and at least one membrane cartridge disposed in the at least one membrane sub-unit, the at least one membrane cartridge having a non-cylindrical profile.

The present disclosure describes a device and method to clear solutes from a patient's blood while maintaining fluid balance. In some implementations, the device and method is used to assist the filtration functions of a patient's liver or kidney. The device includes a plurality of hollow fibers that pass through a sequence of alternating filtration chambers and infusion chambers. The filtration chambers filter the patient's blood while the infusion chambers rehydrate the filtered blood.