Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.

Provided is a foam discharger including: a mixing portion for mixing a liquid agent and a gas to foam the liquid agent; a discharge opening for discharging the foamed liquid agent; and a flow path in communication with the discharge opening, and for supplying the foamed liquid agent from the mixing portion to the discharge opening. The discharge opening is provided with a first porous member. On an upstream side of the first porous member, a cross-sectional area of the flow path on a cross section orthogonal to a supply direction in which the foamed liquid agent is to be supplied increases along the supply direction. The cross-sectional area of the flow path at the discharge opening is at least 1.2 times the minimum cross-sectional area of the flow path.

A substrate processing apparatus includes a chamber, a manifold including a tubular portion above the chamber, first and second introduction pipes provided on a side surface of the tubular portion, and a gas guide portion to guide, in a direction opposite the chamber, gases introduced from the first and second introduction pipes into the tubular portion, and then introduce the gases into the chamber. The gas guide portion does not contact a top of the manifold, and the manifold includes a space above the gas guide portion to allow the gases to flow from between the gas guide portion and the tubular portion into a space surrounded by the gas guide portion. The gas guide portion advantageously enables the gases to broadly diffuse and uniformly mix, increasing the quality of a film formed on a substrate inside the chamber.

A nozzle for spouting a liquid phase, in which one liquid phase in a two-liquid phase system can be stably jetted as highly dispersible droplets while suppressing coalescence of droplets, and accumulation of fine solid components in the nozzle hardly occurs. The nozzle has a structure in which capillary tubes or pores are assembled. Further, the capillary tubes or the pores are formed using a suitable material having a low affinity for organic or a low affinity for water, or a material subjected to appropriate surface treatment.

Provided is a substance mixing apparatus including two or more flow paths in which orifices, from which a fluid that flows therethrough is externally discharged, are formed, oscillation devices that form droplets of the fluid discharged from each of the orifices by oscillating at least the orifice part of the flow paths at a predetermined oscillation frequency and discharge the droplets, and means for causing the droplets discharged from the orifices of the flow paths to collide with one another.

A system and method for producing an oil-in-water (“O/W”) emulsion performs or includes the steps of: a) providing an oil phase and a water phase, b) premixing the oil phase and the water phase to form an O/W pre-emulsion, and c) homogenizing the O/W pre-emulsion to form an O/W emulsion by at least one counter-jet disperser.

A process and device to create access to low gravity solids (LGS) of about 2 to 20 microns for removal from a fluid material/LGS emulsion having the steps of: flowing the emulsion into high pressure tubing; separating the emulsion into at least two high pressure streams; forcing the emulsion through high pressure nozzles at a terminus of each of the at least two high pressure tubing streams at a speed in the range of about 10 ft/sec to 200 ft/sec or at a force in a range of about 10 to 100 PSI; and colliding the streams of emulsion exiting the high pressure nozzle within a pressure drop chamber, wherein the pressure drop is in a range of about 5% to 50% of the back pressure of the nozzles; wherein a cavitation effect is realized from a collision force of the high pressure streams within the pressure drop chamber.

A gas mixing system for semiconductor fabrication includes a mixing block. The mixing block defines a gas mixing chamber, a first gas channel fluidly coupled to the gas mixing chamber at a first exit location, and a second gas channel fluidly coupled to the gas mixing chamber at a second exit location, wherein the first exit location is diametrically opposite the second exit location relative to the gas mixing chamber and the second gas channel has a bend of 90 degrees or less between an entrance of the second gas channel and the second exit location.

To condition an operating medium in a test object circuit (PK) of a test object (P) on a test bench to a desired temperature as quickly as possible, the invention proposes providing a mixing unit (3), there being provided, in the mixing unit (3), a mixing region (28) in which operating medium of the test object circuit (PK) can be mixed with preconditioned operating medium from a conditioning circuit (KK) in order to condition the operating medium in the test object circuit (PK) to the predefined setpoint temperature (T_SOLL), there being provided, on the mixing unit (3), for the fluidic integration of the mixing unit (3) in the test object circuit (PK), at least one test object circuit supply connection (26a) and at least one test object circuit outlet connection (26b) which are fluidically connected to one another via the mixing region (28) in order to form a part of the test object circuit (PK), there being provided, on the mixing unit (3), for connection of the mixing unit (3) to a conditioning unit (2) of the conditioning system (1), at least one conditioning unit supply connection (27a) and at least one conditioning unit return connection (27b) which are fluidically connected to one another via the mixing region (28) in order to form a part of the conditioning circuit (KK) for the operating medium.

To condition an operating medium in a test object circuit (PK) of a test object (P) on a test bench to a desired temperature as quickly as possible, the invention proposes providing a mixing unit (3), there being provided, in the mixing unit (3), a mixing region (28) in which operating medium of the test object circuit (PK) can be mixed with preconditioned operating medium from a conditioning circuit (KK) in order to condition the operating medium in the test object circuit (PK) to the predefined setpoint temperature (T_SOLL), there being provided, on the mixing unit (3), for the fluidic integration of the mixing unit (3) in the test object circuit (PK), at least one test object circuit supply connection (26a) and at least one test object circuit outlet connection (26b) which are fluidically connected to one another via the mixing region (28) in order to form a part of the test object circuit (PK), there being provided, on the mixing unit (3), for connection of the mixing unit (3) to a conditioning unit (2) of the conditioning system (1), at least one conditioning unit supply connection (27a) and at least one conditioning unit return connection (27b) which are fluidically connected to one another via the mixing region (28) in order to form a part of the conditioning circuit (KK) for the operating medium.