In an embodiment, a tubing module for a bioprocessing system includes a first tubing holder block configured to receive at least one pump tube and hold the at least one pump tube in position for selective engagement with a peristaltic pump, a second tubing holder block configured to receive a plurality of pinch valve tubes and hold each pinch valve tube of the plurality of pinch valve tubes in position for selective engagement with a respective actuator of a pinch valve array, and wherein the first tubing holder block and the second tubing holder block are interconnected.

A bioreactor vessel includes a bottom plate, a vessel body coupled to the bottom plate, the vessel body and the bottom plate defining an interior compartment therebetween, and a plurality of recesses formed in the bottom plate, each recess of the plurality of recesses being configured to receive a corresponding alignment pin on a bed plate for aligning the bioreactor vessel on the bed plate.

An apparatus for bioprocessing includes a housing, a drawer receivable within the housing, the drawer including a plurality of sidewalls and a bottom defining a processing chamber, and a generally open top, the drawer being movable between a closed position in which the drawer is received within the housing, and an open position in which the drawer extends from the housing enabling access to the processing chamber through the open top, and at least one bed plate positioned within the processing chamber and configured to receive a bioreactor vessel.

A mixing apparatus, comprising a drive unit (1) with a drive (10) and a shaft (11) which is pivotable about a pivot axis (A) and which is operatively connected to the drive (10), a pivot receptacle (2) which is arranged on the shaft (11), wherein the pivot axis (A) is substantially horizontally aligned, wherein the pivot receptacle (2) comprises a base (20) on which receptacles (21, 22) are provided which extend away from the base (20) and into which holders (3) for containers (7) are insertable.

The present invention relates to a novel bioreactor system for cell cultivation. More specifically, the invention relates to a compact bioreactor system which has several integrated functions and enables small scale static culture as well as scale-up rocking culture in the same bioreactor. The bioreactor system comprises tray for positioning of a cell culture bag having adjustable volume, a lid covering the cell culture bag and provided with heating function, an integrated perfusion unit, an integrated cell loading unit, and an integrated unit for automatic cell culture sampling, wherein the bioreactor system is controlled by a single control unit. The invention also relates to a method of cell culture using the bioreactor system for culture of therapeutic cells.

Layering of initially mixed granular dissimilar material components deposited by surface flow is realized by varying the relative angle of the flowing layer periodically with respect to the bulk of the pile formed. Layer thickness and extent can be controlled by varying the timing and extent of the surface variations. Variation in surface flow rates may be realized by varying the feed rate from an inlet to a collection base at a fixed feed location, varying the feed location relative to the collection base, tilting the collection base that is supporting the material, and reducing the pile repose angle using vibration or fluid flow through the base.

A system for bioprocessing includes a tray having plurality of sidewalls and a bottom surface defining an interior compartment, and a generally open top, and an opening formed in the bottom surface of the tray, the opening having a perimeter. The perimeter of the opening is shaped and/or dimensioned such that a bioreactor vessel can be positioned above the opening and supported by the bottom surface of the tray while a portion of the bioreactor vessel is accessible through the opening in the bottom surface. The tray is receivable within a processing chamber such that the bed plate extends through the opening in the bottom surface of the tray to support the bioreactor vessel.

A mixing machine includes a mixing head and at least one connection means for connecting a mixing container containing material to be mixed to said mixing head for forming a closed mixing container. The mixing head, as part of a pivotable assembly, is pivotably mounted with respect to a frame such that the closed mixing container formed from the mixing head and the mixing container can be pivoted relative to the frame for performing the mixing process. The mixing head carries at least one rotationally-driven mixing tool. The mixing head comprises a head plate having a connecting flange molded thereon which is configured as an annular disc and comprises a planar contact surface. At least two ring seals of differing diameters are arranged in said contact surface of the connecting flange at a spacing between each other, such that mixing containers with different connection diameters of their mixing head side can be connected to said mixing head. The at least one connection means is configured for gripping mixing containers which differ in the diameter of their connection sides.

A method for enhancing mixing and aeration of a liquid reaction medium in a toroidal bioreactor vessel includes dispensing a liquid reaction medium into an interior of a toroidal bioreactor vessel, the interior being bounded by an inner surface, a textured surface being arranged on at least a portion of the inner surface, the textured surface having a plurality of upstanding protuberances. The toroidal bioreactor vessel is rotated in an orbital motion such that there is a resonant frequency traveling wave of the fluid orbiting in one direction in the interior of the toroidal bioreactor vessel when a particular orbital speed is imparted to the toroidal bioreactor vessel.

A non-transitory computer readable medium includes instructions configured to adapt a controller to maintain a first target environment in a bioreactor vessel containing a population of cells for a first incubation period to produce a population of genetically modified cells from the population of cells, initiate a flow of media to the bioreactor vessel, maintain a second target environment in the bioreactor vessel for a second incubation period to produce an expanded population of genetically modified cells.