Provided herein are compositions, systems, and methods for high throughput screening. In particular, provided herein are microfluidic devices for high throughput analysis of multiplex chemical (e.g., drug interactions) across a wide range of concentrations.

The present invention provides an improved process for lipid nanoparticle formulation and mRNA encapsulation. In some embodiments, the present invention provides a process of encapsulating messenger RNA (mRNA) in lipid nanoparticles comprising a step of mixing a suspension of preformed lipid nanoparticles and mRNA.

An exemplary pharmaceutical compounding system and device for mixing materials from at least two distinct material sources can include a transfer set and junction structure that has a junction body, a first inlet port located at a first portion of the junction body, a second inlet port located at a second portion of the junction body, and an outlet port located at a third portion of the junction body. The junction structure can be configured to mix fluid received from both the first inlet port and second inlet port and to deliver the fluid to the outlet port. The junction structure can also include attachment structure located on the junction body and configured to attach the junction structure to the housing of the compounding device at a location downstream of a pump system.

The present invention provides a fluidic device in which solutions of different concentrations can be easily obtained. The fluidic device includes: a first substrate and a second substrate which are stacked in a thickness direction; an undiluted solution introduction flow path which has an undiluted solution introduction port and which is constituted of a groove part provided on at least one of the first substrate and the second substrate; a first circulation flow path which is constituted of a groove part having an annular shape and having a shared part that shares part of a flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; a second circulation flow path which is provided independently of the first circulation flow path and which is constituted of a groove part having an annular shape and having a shared part that shares some flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; and/or a third circulation flow path which is constituted of a groove part having an annular shape and having a shared flow path that shares part of a flow path with the first circulation flow path and a non-shared flow path which is not shared with the first circulation flow path and which is connected to a diluting solution introduction port, wherein the undiluted solution introduction flow path includes a valve at both ends of the shared part.

A microfluidic chip and a cosmetic manufacturing apparatus including the microfluidic chip are disclosed. The microfluidic chip contains an inlet portion including a first and a second fluid inlets; a dissolution portion including a first and a second micro flow paths in which a first and a second modular raw materials dissolved by the first and the second fluids are pre-loaded, respectively; a confluence including a third micro flow path in which the first and the second fluids and the first and the second modular raw materials converge; a stir portion including a fourth micro flow path which forms a mixed fluid by mixing the first and the second fluids and the first and the second modular raw materials; and a vortexing portion configured to form a cosmetic composition.

A microfluidic chip and a cosmetic manufacturing apparatus including the microfluidic chip are disclosed. The microfluidic chip contains an inlet portion including a first and a second fluid inlets; a dissolution portion including a first and a second micro flow paths in which a first and a second modular raw materials dissolved by the first and the second fluids are pre-loaded, respectively; a confluence including a third micro flow path in which the first and the second fluids and the first and the second modular raw materials converge; a stir portion including a fourth micro flow path which forms a mixed fluid by mixing the first and the second fluids and the first and the second modular raw materials; and a vortexing portion configured to form a cosmetic composition.

According to one embodiment, a fluid controller includes a fluid channel deforming portion and a mixing portion provided downstream from the fluid channel deforming portion. The fluid channel deforming portion includes an upstream end portion, a first channel, a second channel and a channel terminating portion. At least one of the first and second channels is deformed between the upstream end portion and the channel terminating portion. A region of the second channel in a second cross-section, is increased more than a region of the second channel in the first cross-section, between the upstream end portion ad the channel terminating portion. The mixing portion mixes a plurality of fluids flowing through the fluid channel deforming portion.

The present invention relates to compositions and methods for manufacturing an adjuvant comprising a saponin using a microfluidic device and to aspects thereof.

A microfluidic module for co-encapsulation in droplets of two populations of particles may include first and second modules for sorting the two populations. The modules may have their first outlets including first obstructive valves configured to at least partially obstruct the first outlets. The first outlets may be fluidly connected to a fusion module, including a fusion module means for merging at least one droplet from the first droplet population and at least one droplet from the second droplet population into a merged droplet comprising the two population of particles, and a control unit for controlling the first obstructive valves from information originating from a first and second module detection portion located upstream of the first outlets.

A microfluidic module for co-encapsulation in droplets of two populations of particles may include first and second modules for sorting the two populations. The modules may have their first outlets including first obstructive valves configured to at least partially obstruct the first outlets. The first outlets may be fluidly connected to a fusion module, including a fusion module means for merging at least one droplet from the first droplet population and at least one droplet from the second droplet population into a merged droplet comprising the two population of particles, and a control unit for controlling the first obstructive valves from information originating from a first and second module detection portion located upstream of the first outlets.