The invention relates to a chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing comprising: a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing, and a continuous medium permeable to molecular diffusion extending between said ducts, comprising a porous organic gel or an organic liquid and including at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated and opening to the ducts, so as to give said at least one species a diffusive path between said ducts. The invention also relates to a packing for the implementation of such a method and a method for manufacturing such a packing.

The invention relates to a chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing comprising: a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing, and a continuous medium permeable to molecular diffusion extending between said ducts, comprising a porous organic gel or an organic liquid and including at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated and opening to the ducts, so as to give said at least one species a diffusive path between said ducts. The invention also relates to a packing for the implementation of such a method and a method for manufacturing such a packing.

The invention relates to a curable composition, containing (A) at least one polyorganosiloxane, which has at least one hydroxy group bound to a silicon atom, (B) at least one silane of the formula (1):

Si(R.sup.1).sub.m(R.sup.2).sub.n(R.sup.3).sub.4-(m+n)(1), as defined herein, (C) at least one aminosilane, and (D) at least one tin compound,
wherein the composition is free of epoxysilane and the molar ratio of the aminosilane to the tin compound is 1:1 to 50:1, as well as to the preparation and use thereof.

Methods, compositions, devices and kits having a novel chromatographic material are provided herein for separating and identifying organic molecules and compounds, for example molecules and compounds containing electron rich functional groups such as carbon-carbon double bonds. The methods, compositions, and kits include a metal-thiolate chromatographic medium (MTCM) with a sulfur-containing functional group or a metal-selenolate chromatographic medium (MSCM) comprising a selenium-containing functional group covalently attached to a support medium, such that the sulfur-containing functional group or selenium-containing functional group is bound to at least one metal atom. The MTCM and/or MSCM has affinity and specificity to compounds having one or more carbon-carbon double bonds, and performs a highly efficient and rapid separation of samples yielding non-overlapping peaks of purified materials compared to traditional media.

Methods, compositions, devices and kits having a novel chromatographic material are provided herein for separating and identifying organic molecules and compounds, for example molecules and compounds containing electron rich functional groups such as carbon-carbon double bonds. The methods, compositions, and kits include a metal-thiolate chromatographic medium (MTCM) with a sulfur-containing functional group or a metal-selenolate chromatographic medium (MSCM) comprising a selenium-containing functional group covalently attached to a support medium, such that the sulfur-containing functional group or selenium-containing functional group is bound to at least one metal atom. The MTCM and/or MSCM has affinity and specificity to compounds having one or more carbon-carbon double bonds, and performs a highly efficient and rapid separation of samples yielding non-overlapping peaks of purified materials compared to traditional media.

Disclosed is a method for the determination of impurities in polyalkylene ethers and polyalkylene amines comprising the steps i) introducing polyalkylene ethers or polyalkylene amines as an analyte into a chromatography column containing monolithic silica gel as a stationary phase, ii) eluting the analyte with a liquid elution agent having such a polarity that the analyte is in adsorptive equilibrium with the stationary phase during chromatography, iii) detecting the components of the analyte at the exit-side end of the chromatography column, receiving a chromatogram, which shows different components of the analyte and its qualitative amount depending on the elution time of the individual components, and iv) identifying bands in the chromatogram having a low height or area compared to the band with the largest height or area as an indication of the presence of impurities in the analyte.

The method allows in an easy manner to identify impurities in the sample. The method can be used for quality control but also for the preparative cleaning of the sample.

Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.

The present invention provides a method for hydrophobization of a hydrophilic material, the method including introducing a hydrophobic group into a hydroxyl group (OH group) on a surface of the hydrophilic material. A method for hydrophobization of a hydrophilic material, the method comprising reacting a hydrophilic material to be hydrophobized with a hydrophobic group-containing silylating agent in presence of an amino acid as a reaction accelerator, to introduce a hydrophobic group-containing silyl group to a surface of the hydrophilic material. A hydrophobized silica gel column filler is produced by using the method. Further, a hydrophobized silica gel column is produced by filling a column with the hydrophobized silica gel column filler.

The present invention provides a method for hydrophobization of a hydrophilic material, the method including introducing a hydrophobic group into a hydroxyl group (OH group) on a surface of the hydrophilic material. A method for hydrophobization of a hydrophilic material, the method comprising reacting a hydrophilic material to be hydrophobized with a hydrophobic group-containing silylating agent in presence of an amino acid as a reaction accelerator, to introduce a hydrophobic group-containing silyl group to a surface of the hydrophilic material. A hydrophobized silica gel column filler is produced by using the method. Further, a hydrophobized silica gel column is produced by filling a column with the hydrophobized silica gel column filler.

A chromatography column has a retaining plug permanently fixed to an upstream end of the column and blocks one end of the bore through the column. The plug has a fluid passage therethrough. An upstream end of the passage is preferably but optionally larger in diameter than a downstream end of the passage. An upstream porous member upstream of the retaining plug is held by an upstream end cap and urged toward the plug. Chromatographic media extends from the upstream porous member, through the passage in the retaining plug, to a downstream porous member held by a downstream end cap. The media between the retaining plug and the downstream porous member are under compression to form a bed of packed media.