The present invention relates to chromatography beads, production and use thereof. More closely the invention relates to small, rigid and nan-permeable agarose beads suitable for example as stationary phase in high performance liquid chromatography (HPLC) for analyses of biomolecules, such as, peptides and proteins; and methods for producing such beads.

A conductive composition comprising a conductive polymer (A) having an acidic group, and a basic compound (B), wherein: an area ratio (X/Y) is 0.046 or less as calculated by an specific method, which is a ratio an area (X) of a region corresponding to molecular weight (M) ranging from 300 to 3300, relative to an area (Y) of an entire region ascribed to the conductive polymer (A); or a ratio, ZS/ZR, is 20 or less, wherein the ZS is a maximum value of fluorescence intensity in a wavelength region of 320 to 420 nm when a fluorescence spectrum is measured using a spectrofluorometer at an excitation wavelength of 230 nm with respect to a measurement solution obtained by diluting the conductive composition with water so as to adjust solids content of the conductive polymer (A) to 0.6% by mass, and the ZR is a maximum value of Raman scattering intensity in a wavelength region of 380 to 420 nm when a fluorescence spectrum of water is measured using a spectrofluorometer at an excitation wavelength of 350 nm.

A belt drier arrangement for drying an aqueous polymer gel and for comminuting the dried polymer gel to give dried polymer particles, having: a belt drier having a drier setup and a conveyor belt for receiving and for drying the aqueous polymer gel to give a dry cake or fragments on a receiving surface of the conveyor belt, and a comminution arrangement, disposed downstream of the drier setup in product flow direction, for comminution of the dried polymer gel of the dry cake or fragments to give dried comminuted polymer particles. The comminution arrangement has a comminutor in the form of a milling drum which is disposed with an upper working line of a working edge of the milling drum at the level of or above a receiving surface of a conveyor belt, for mill processing of the dry cake or of the fragments, where the milling drum is designed to eject comminuted dried polymer particles of the dry cake or of the fragments in the downward direction.

A belt drier arrangement for drying an aqueous polymer gel and for comminuting the dried polymer gel to give dried polymer particles, having: a belt drier having a drier setup and a conveyor belt for receiving and for drying the aqueous polymer gel to give a dry cake or fragments on a receiving surface of the conveyor belt, and a comminution arrangement, disposed downstream of the drier setup in product flow direction, for comminution of the dried polymer gel of the dry cake or fragments to give dried comminuted polymer particles. The comminution arrangement has a comminutor in the form of a milling drum which is disposed with an upper working line of a working edge of the milling drum at the level of or above a receiving surface of a conveyor belt, for mill processing of the dry cake or of the fragments, where the milling drum is designed to eject comminuted dried polymer particles of the dry cake or of the fragments in the downward direction.

A compound having the formula (I): ##STR00001##
is disclosed. Methods of preparing the compound of formula (I) are also disclosed. The compound of formula (I) can be prepared by reacting a compound of formula (II) with a compound of formula (III) in an organic solvent with EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide). The compound of formula (I) can also be prepared by reacting the compound of formula (II) with the compound of formula (III) in 1-ethyl-3-methylimidazolium hexafluorophosphate as a solvent with silicomolybdic acid as a catalyst.

A compound having the formula (I): ##STR00001##
is disclosed. Methods of preparing the compound of formula (I) are also disclosed. The compound of formula (I) can be prepared by reacting a compound of formula (II) with a compound of formula (III) in an organic solvent with EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide). The compound of formula (I) can also be prepared by reacting the compound of formula (II) with the compound of formula (III) in 1-ethyl-3-methylimidazolium hexafluorophosphate as a solvent with silicomolybdic acid as a catalyst.

A chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing. The packing includes a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing. A continuous medium permeable to molecular diffusion extends between the ducts, including a porous organic gel or an organic liquid with at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated. The at least one species has a diffusive path between the ducts.

A chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing. The packing includes a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing. A continuous medium permeable to molecular diffusion extends between the ducts, including a porous organic gel or an organic liquid with at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated. The at least one species has a diffusive path between the ducts.

Disclosed are composite materials and methods of making them. The composite materials comprise a support member and a cross-linked gel, wherein the cross-linked gel is a polymer synthesized by thiol-ene or thiol-yne polymerization and cross-linking. The cross-linked gel may be functionalized by a thiol-ene or thiol-yne grafting reaction, either simultaneously with the polymerization or as the second step in a two-step procedure. The composite materials are useful as chromatographic separation media.

Disclosed are composite materials and methods of making them. The composite materials comprise a support member and a cross-linked gel, wherein the cross-linked gel is a polymer synthesized by thiol-ene or thiol-yne polymerization and cross-linking. The cross-linked gel may be functionalized by a thiol-ene or thiol-yne grafting reaction, either simultaneously with the polymerization or as the second step in a two-step procedure. The composite materials are useful as chromatographic separation media.