Chromatographic processes are provided which utilize fiber conduit contactors to effect separation of chemical substances from a mixture. In particular, processes are provided which constrain a substance on the fibers and move a mixture of chemical substances and another fluid through the coated fibers to effect separation of a substance from the mixture. In addition, fiber conduit contactors configured to affect such processes are disclosed. Some apparatuses include a sample injection mechanism for selectively inserting an analytical or preparative chromatography mixture into a fluid being supplied to the conduit. Additional or alternative apparatuses include fibers positioned longitudinally within a conduit with their opposing ends respectively bundled into a fluid inlet and a fluid outlet at opposing ends of the conduit. The portions of the fibers between the bundled ends are sufficiently slack such that larger spaces exist between individual fibers along the slack portions than at the bundled ends.

An apparatus for treating an aqueous sample stream includes analyte ions. The apparatus comprises an ion exchange barrier; a sample stream flow channel; an ion receiving stream flow channel adjacent to the sample stream flow channel and separated therefrom by said first ion exchange bather. Stationary flow-through ion exchange packing is disposed in the sample flow channel of the same charge as the ion exchange bather. The ion exchange packing comprises a mixture of a first ion exchange portion with strong ionizable groups and a second ion exchange portion with weak ionizable groups of the same charge. First and second electrodes are in electrical communication with the sample stream flow channel and ion receiving flow channel.

To provide a method for separating and quantifying saturated and unsaturated dialkyl ketones. (1) A method for separating and quantifying saturated and unsaturated dialkyl ketones, the method including quantifying a dialkyl ketone-containing sample by a gas chromatograph using a polar column, except a nonpolar column and a slightly polar column, the polar column having a polarity value of 440 or greater. (2) The method according to (1), wherein the polar column is a polar column with medium polarity or higher polarity, the polar column having a polarity value of 700 or greater. (3) The method of (2), wherein the polar column is a polar column with high polarity or higher polarity, the polar column having a polarity value of 1550 or greater. (4) The method according to (3), wherein a liquid phase of the polar column with high polarity or higher polarity is an ionic liquid column. (5) A method for producing a chemical transesterified oil and/or fat, including separating and quantitatively analyzing saturated and unsaturated dialkyl ketones in an oil and/or fat, using the method described in any one of (1) to (4).

To provide a method for separating and quantifying saturated and unsaturated dialkyl ketones. (1) A method for separating and quantifying saturated and unsaturated dialkyl ketones, the method including quantifying a dialkyl ketone-containing sample by a gas chromatograph using a polar column, except a nonpolar column and a slightly polar column, the polar column having a polarity value of 440 or greater. (2) The method according to (1), wherein the polar column is a polar column with medium polarity or higher polarity, the polar column having a polarity value of 700 or greater. (3) The method of (2), wherein the polar column is a polar column with high polarity or higher polarity, the polar column having a polarity value of 1550 or greater. (4) The method according to (3), wherein a liquid phase of the polar column with high polarity or higher polarity is an ionic liquid column. (5) A method for producing a chemical transesterified oil and/or fat, including separating and quantitatively analyzing saturated and unsaturated dialkyl ketones in an oil and/or fat, using the method described in any one of (1) to (4).

An analysis method for mycotoxins including a separation step, a detection step, and an identification step. In the separation step, each component contained in a liquid sample is separated in a column In the detection step, components separated in the separation step are detected by a PDA and a fluorescence detector. In the identification step, total aflatoxin is identified based on a detection signal from the fluorescence detector, and deoxynivalenol is identified based on a detection signal from the PDA.

The invention relates to a chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing comprising: a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing, and a continuous medium permeable to molecular diffusion extending between said ducts, comprising a porous organic gel or an organic liquid and including at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated and opening to the ducts, so as to give said at least one species a diffusive path between said ducts. The invention also relates to a packing for the implementation of such a method and a method for manufacturing such a packing.

The invention relates to a chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing comprising: a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing, and a continuous medium permeable to molecular diffusion extending between said ducts, comprising a porous organic gel or an organic liquid and including at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated and opening to the ducts, so as to give said at least one species a diffusive path between said ducts. The invention also relates to a packing for the implementation of such a method and a method for manufacturing such a packing.

The invention discloses a coupled peptide chain for dissolving poorly soluble polypeptides and an application thereof for separation and purification in liquid chromatography, belonging to the field of biochemistry. A special linker arm is used to link a hydrophilic polypeptide chain with a poorly soluble polypeptide chain to solve the problem that the poorly soluble polypeptide chains cannot be operated in the liquid chromatography, and optimize the combination of hydrophilic amino acids, and then the poorly soluble polypeptide chain and hydrophilic polypeptide chain are broken by hydrolyzing an ester bond, so that the target peptide chain is directly precipitated, the method has the characteristics of simplicity and high efficiency, and the poorly soluble polypeptide product obtained by the method fully meets the standards required by customers.

The invention discloses a coupled peptide chain for dissolving poorly soluble polypeptides and an application thereof for separation and purification in liquid chromatography, belonging to the field of biochemistry. A special linker arm is used to link a hydrophilic polypeptide chain with a poorly soluble polypeptide chain to solve the problem that the poorly soluble polypeptide chains cannot be operated in the liquid chromatography, and optimize the combination of hydrophilic amino acids, and then the poorly soluble polypeptide chain and hydrophilic polypeptide chain are broken by hydrolyzing an ester bond, so that the target peptide chain is directly precipitated, the method has the characteristics of simplicity and high efficiency, and the poorly soluble polypeptide product obtained by the method fully meets the standards required by customers.

In accordance with embodiments of the present invention, a terpene-rich sample is prepared for terpene analysis using liquid chromatography via an extraction method that takes little time, uses minimal external equipment, and permits direct injection of extracted terpenes into a liquid chromatography instrument for analysis. An embodiment of the invention involves preparing a terpene-containing sample for analysis by liquid chromatography by liquid extraction; heating the liquid extract in a vial that contains a filter medium or solvent; collecting the terpenes in the medium by the vapor pressure forced through the filter from heating; and eluting the collected terpenes into a vial or directly into a chromatography injector.