A method for purifying a substance in a solution in a simple streamlined process using a magnetic porous particle. For easy small scale purification of a substance, the magnetic porous particle is coated with either a hydrophilic or hydrophobic liquid and transferred into a second liquid containing the substance under conditions which allow said substance to partition into the first liquid within said magnetic porous particle. Finally the magnetic porous particle is removed from said second liquid, wherein the first and second liquid are substantially immiscible and the partition coefficient P of the substance between the first and second liquid is greater than 1.

Bi-structured matrix for purification and handling of solid reagents, which comprises at least a polymer solid carrier coated with at least one hydrosoluble polymer, and manufacturing processes. The solid carrier may be, among others, cross-linked polyurethane foam or a micropipette tip. The hydrosoluble polymer may be, among others, polyvinylalcohol, agarose, hydroxyethylcellulose or combinations thereof. The matrix may further comprise a polymer produced from monomers of glycidyl metacrylate (GMA), dimethyl acrylamide (DMAAm), 2-hydroxyethyl metacrylate, metacrylic acid, or combinations thereof.

Methods, kits and devices for separating phospholipids and proteins from small molecules in biochemical samples can feature an apparatus having a wetting barrier, at least one fit and a separation media. For example, an apparatus can include at least one wall defining a chamber having an exit and an entrance; a wetting barrier disposed between the exit and entrance, so as to define a separation media space located between the wetting barrier and the exit and a sample receiving area located between the wetting barrier and the entrance; and a separation media disposed adjacent to the wetting barrier and having a specific affinity for phospholipids. The wetting barrier is adapted to (i) retain the liquid sample and a protein precipitating agent in the sample receiving area under a first force, thereby facilitating the formation of a protein precipitate and a processed sample, and (ii) flow the processed sample through the wetting barrier and separation media under a second force, wherein the second force is greater than the first force, thereby retaining the protein precipitate in the sample receiving area, retaining phospholipids in the separation media, and eluting small molecules.

Certain aspects and examples are directed to sorbent devices and methods of using them. In certain embodiments, a sorbent device comprising a body comprising a sampling inlet, a sampling outlet and a cavity between the inlet and the outlet, the cavity comprising a serial arrangement of at least four different sorbent materials is described. In some embodiments, the sorbent materials are arranged from a material with a weakest sorbent strength to a material with a strongest sorbent strength with the weakest sorbent strength material adjacent to the sampling inlet.

Proposed are an extracellular vehicle-isolating composition including a homobifunctional hydrazide and a solid support functionalized with amine groups, and a method of isolating extracellular vesicles by using the composition. The method may isolate highly enriched high-purity EVs from a biological sample and also enable simultaneous operation for EVs isolation and extraction of EV-derived proteins or EV-derived nucleic acids. The method is low-cost and simple, does not require special equipment other than a syringe filter, and allows for high-throughput experiments due to the use of large-volume samples. The method may reduce the time required for protein and nucleic acid extraction by simplifying the experiments. Additionally, this EV enrichment may increase the sensitivity of biomarker detection for disease diagnosis and treatment.

The invention relates to membranes for separation, removal, and/or concentration purposes. The object of the invention is the simple and reliable adsorption of the molecules and to simplify the desorption of target molecules that are adsorbed and chromatographically bonded on membranes, preferably without the addition of substances with a high ion content, such as acids, alkalis or salts. The object of the invention is also to develop a value that can be easily measured, which allows for an indication of the current and/or remaining binding capacity of the membrane during the adsorption process and/or the control thereof. The adsorption takes place on a charged membrane and desorption is achieved using physical, electromagnetic and/or the generation of electrical fields. This is carried out with a thin metal layer being applied to one or both sides of a positively or negatively charged membrane and a voltage is applied for desorption.

The present disclosure is directed to surface modified materials such as stationary phase materials for performing size exclusion chromatography. Aspects of the present disclosure feature materials surface modified with a moiety including a polyethylene glycol (PEG) functionality and a moiety comprising a diol functionality. Such surface modified materials exhibit a reduced propensity for ionic and hydrophobic secondary interactions.