The microfluidic device capable of initiating sequential flow according to the present invention includes: a main flow path in which a suction port for sucking the fluid with a negative pressure is formed at one end; a plurality of reservoirs that supply a fluid stored therein to the main flow path through an outlet by the negative pressure applied to the suction port, and are connected to a plurality of different points of the main flow path; and a blocking element that blocks the inflow of external air to the main flow path through the outlet when all the fluid in the reservoir flows out, wherein the fluid stored in a plurality of the reservoirs may flow sequentially.

A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

The present invention relates to a lab-on-a-chip (LOAC)-system for the rapid detection of e.g. pathogens. The system comprises a tabletop detection apparatus and a portable optical detection cartridge for being received in the inner of the detection apparatus, the cartridges comprising a plurality of test wells for detecting a desired chemical reaction taking place within a respective test well. In embodiments of the invention, the optical detection cartridge is pre-loaded with suitable respective reagents selective for a disease pathogen such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

An automatic chemical solution formulating device combines and mixes stored solids and liquids into user specified formulations and dispenses those formulations into containers. Chemical solids are stored in cartridges of material separated into predetermined dosages (for example in reeled blister packs), avoiding the need for weighing during formulation. Elements include user interface, computer-controlled automated loading and unloading port for reagent-containing cartridges, cartridge conveyor system, reader for identifying cartridges, blister-pack strip drive system, punching mechanism to release reagents, portioning chamber to mix solvent with solids or liquids with optional portioning, accommodating formulation delivery port, position sensors, liquid flow measuring devices, liquid and gas pumps and valves, and label printer. The combination of these elements allows high-speed formulation and dispensing of user-specified formulations.

A substrate has a plurality of microdots positioned thereon. Each microdot contains one or more primers for gene amplification for a particular target gene. The microdots are placed on the substrate and the substrate is positioned in a housing. The housing has a sample fluid to be tested introduced therein covering the microdot array. While the sample fluid is overlying the substrate, the amplification of the target gene is carried out if it is present within the sample. If the target gene that matches the primers is not present, then amplification will not take place. The fluid also contains fluorophores which will be fixed into the gene as it increases in size as it clearly detects if gene amplification has occurred by detecting the amount of light detected for a particular microdot. In a preferred embodiment, the sample fluid is placed on top of a sealing layer that is less dense then water, such as wax or mineral oil. During a heating of the sample fluid and sealing layer, the sample fluid will sink to the bottom of the sealing layer so that it is fully encased and protected.

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.

The invention relates to a standard solution for inverse gas chromatography and/or surface energy analysis; a volumetric container for preparing such a standard solution; a method of preparing such a standard solution for inverse gas chromatography and/or a surface energy analysis and a method of probing a solid sample. The standard solution comprises a series of three or more compounds of increasing carbon chain length of general formula (I): R—X wherein: for the three or more compounds R is a series of alkyl, a series of alkenyl or a series of alkynyl groups of increasing carbon chain length; and for all three or more compounds X is H, OH, CO.sub.2H, C(O)H, C(O)CH.sub.3, NH.sub.2, SH or halogen; and the relationship between carbon chain length and volume of the compounds of increasing carbon chain length of general formula (I) is determined by the following formula.

A biological material manufacturing device according to an embodiment of the inventive concept includes a main body and a head unit that is rotatable on the main body. The main body includes a main groove and a first container groove connected to the main groove. The head unit includes a pillar provided in the main groove and a protruding part that protrudes from the pillar.

A liquid handling device includes a plurality of first wells configured for a first sample; a first channel connected to the plurality of first wells; a plurality of second wells configured for a second sample; a second channel connected to the plurality of second wells; a plurality of processing agent wells configured for a processing agent configured to process the first sample and the second sample; a processing agent channel connected to the plurality of processing agent wells; and a common channel connected to the first channel, the second channel and the processing agent channel.

A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir, (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.