A microfluidic, chip-based assay device has been developed for measuring physical properties of an analyte (particularly, whole blood or whole blood derivatives). The technologies can be applied to measure clotting times of whole blood or blood derivatives, determine the effects of anticoagulant drugs on the kinetics of clotting/coagulation, as well as evaluate the effect of anticoagulant reversal agents. These technologies can additionally be used to optimize the dosage of anticoagulation drugs and/or their reversal agents. The assay is independent of the presence of anticoagulant; clotting is activated by exposure of the blood sample in the device to a glass (or other negatively charged material such as oxidized silicon) surface, which activates the intrinsic pathway and can be further hastened by the application of shear flow across the activating materials surface. The absence of chemical activating agents and highly controlled and reproducible micro-environment yields a point of care universal clotting assay.

A microfluidic component package that is readily integratable within a microfluidic system.

An apparatus for performing microfluidic-based biochemical assays, the apparatus includes a microfluidic device, wherein the microfluidic device comprises at least a microfluidic feature comprising at least a reservoir configured to contain at least a fluid, and at least an alignment feature for positioning and attaching a sensor device, wherein the at least an alignment feature is not contacting the at least a microfluidic feature, at least a sensor device configured to be in sensed communication with the at least a fluid and detect at least a sensed property, and at least a flow component fluidically connected to the at least a microfluidic feature configured to flow the at least a fluid through the at least a sensor device.

Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.

Lysis devices, methods, and systems are disclosed including a lysis device comprising a sample vessel having an outer surface, a microchannel within the confines of the outer surface, a first port extending through the outer surface to the microchannel, and a second port extending through the outer surface to the microchannel; and an acoustic transducer bonded to the outer surface of the sample vessel to form a monolithic structure, the acoustic transducer configured to emit ultrasonic acoustic waves into and/or to induce shear forces into a blood sample within the microchannel, thereby rupturing the blood cells.

A modular fluidic chip includes a body configured to have at least one flow channel formed in an inside thereof and be connected to another modular fluidic chip to allow the at least one flow channel to communicate with a flow channel provided in the other modular fluidic chip. A fluidic chip capable of performing one function is formed in the form of a module, whereby a fluidic flow system of various structures can be implemented without restriction in shape or size by connecting a plurality of fluidic chips capable of performing different functions as necessary. Through this, various and accurate experimental data can be obtained, and when a specific portion is deformed or damaged, only the fluidic chip corresponding thereto can be replaced, thereby reducing manufacture and maintenance costs.

This specimen treatment chip includes: a first fluid module having a first flow channel for performing a first treatment step on an object component in a specimen; a second fluid module having a second flow channel for performing a second treatment step on the object component subjected to the first treatment step; a substrate; and a connection flow channel for connecting the first fluid module and the second fluid module disposed on the substrate.

An analysis device includes a guide-in section, a placement section, an illumination member, a pressing member, and a measurement member. The guide-in section is configured to guide a rectangular block shaped analysis kit containing a sample. The analysis kit is placed on the placement section in the guide-in section. The illumination member is inserted into an insertion hole formed in the analysis kit, contacts a bottom of the insertion hole, and illuminates light onto the sample. The pressing member is configured by a separate body to the illumination member and presses the analysis kit such that the analysis kit placed on the placement section is sandwiched between the pressing member and the placement section and retained at a predetermined position. The measurement section measures a component present in the sample using light illuminated from the illumination member onto the sample in the analysis kit placed on the placement section.

Embodiments include systems for sequencing a biological sample. The system may include a reusable subsystem and a removable subsystem. The reusable subsystem may actuate and operate the removable subsystem to automate the sequencing. A base unit of the reusable subsystem may form a fluidic connection between an integrated reagent cartridge and an integrated sensor cartridge of the removable subsystem. The integrated reagent cartridge may be configured to hold reagents and the integrated sensor cartridge may be configured with a biosensor for sequencing the biological sample.

Assay cartridges are described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain embodiments, these cartridges are adapted to receive and analyze a sample collected on an applicator stick. Also described are kits including such cartridges and a cartridge reader configured to analyze an assay conducted using an assay cartridge.