The invention provides a versatile sensing platform for sensing and analysis of biofluids, particularly well-suited for sensing and analysis of sweat. Systems of the invention allows for sensitive and selective detection of a range of analytes in sweat including metabolites, electrolytes and biomarkers. Systems of the invention provide a noninvasive and accurate means for quantitative characterization of important sweat characteristics including sweat volume, sweat loss and sweat rate. Systems of the invention are compatible with materials and device geometries for important class of conformal tissue mounted electronic devices, including epidermal electronic devices.

A system and method for performing a portable, fast, field assay of a small sample biological analyte includes a microfluidic cartridge and a reader with which the microfluidic cartridge is selectively communicated. A closed microfluidic circuit mixes and recirculates the analyte with a buffer. A shear horizontal surface acoustic wave (SAW) detector communicates with the microfluidic circuit and has a plurality of channels including at least one functionalized sensing channel in which the mixed analyte and buffer is recirculated and sensed. Capture of the analyte is amplified by recirculation of the analyte and buffer, and detection is amplified by use of an all-purpose endospore display mass amplification.

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

A detection kit for quickly detecting a lead content lead a sample is provided, and belongs to the technical field of medical in vitro immunoassay. The kit includes a detection card and a quality control; the detection card includes a bottom plate, and a sample pad, a glass fiber membrane, a nitrocellulose membrane and an absorbent paper which are arranged on the surface of the bottom plate sequentially from a loading end. The provided kit has advantages of having simple preprocessing, having no need of digestion, and being economic, fast and convenient, and the like, can realize storage at room temperature, a rapid speed, a high throughout, a low instrument cost, simple and convenient operations, single-person packaging, and detection at any time, which greatly improves the simplicity and convenience of clinical use.

A system for detecting analytes in a test sample, and a method for processing the same, is provided. The system includes a cartridge reader unit that has a control unit and a pneumatic system, and a cartridge assembly that prepares the samples with mixing material(s) through communication channels. The assembly has a memory chip with parameters for preparing the sample and at least one sensor (GMR sensor) for detecting analytes in the sample. The assembly is pneumatically and electronically mated with the reader unit via a pneumatic interface and an electronic interface such that the parameters may be implemented via the control unit. The pneumatic system is contained within the unit and has pump(s) and valve(s) for selectively applying fluid pressure to the pneumatic interface of the assembly, and thus through the communication channels, to move the sample and mixing material(s) through and to sensor. The control unit activates the pneumatic system to prepare the sample and provide it to the sensor for detecting analytes, and also processes measurements from the sensor to generate test results.

Methods of detecting the presence of an analyte in a query sample include providing a sensor that includes biomolecules disposed on a polymer-coated surface of a giant magnetoresistance (GMR) sensor. Modes of operation remove or add magnetic beads from the vicinity of the sensor surface by interactions with the biomolecules. The methods feature detecting the presence of the analyte in the query sample by measuring resistance change of the GMR sensor based on determining resistance before and after passing magnetic particles over the sensor.

A portable isothermal amplification device with a portable heat source and a thermal sensor linking to a cloud environment via mobile devices with a mobile application of the present invention for determining the DNA information of samples with a given primer in a manner of point-of-collection (in the field). The cloud environment with mobile devices can provide isothermal amplification information that may include isothermal amplification related data (e.g., genetic information of organism, cancer cells or viruses of interest), analysis methods, solutions provided by experts for access, storage, analysis and consulting. The information may also include gene expression levels of interest, DNA identity of samples, as well as treatment suggestion and professional lists for consulting. In addition, the cloud environment can provide further solution, which is based on the sequence presence information generated from the devices for the end users.

A portable system for extracting, optionally amplifying, and detecting nucleic acids or proteins using a compact integrated chip in combination with a mobile device system for analyzing detected signals, and comparing and distributing the results via a wireless network. Related systems and methods are provided.

Provided are microfluidic systems for monitoring a biofluid property and related methods. Specially configured microfluidic networks and associated structural support and functional elements, including flexible substrates, capping layers, and fluidic conduits and controllers, provide reliable biofluid collection. Optical components and indicators provide a reliable and readily observable readout, including of any of a number of biofluid properties, including directly from biofluid collected in the microfluidic network.

A smart instrument for mixing (100), a microfluidic chip (50), and a system wherein they are used to prepare formulations is provided. The microfluidic chip comprises microchannels and a programmable data component. The system achieves optimal formulations for RNA, antisense, peptides and small molecules in the hands of even novice users.