Modular active surface devices for microfluidic systems and methods of making the same including adhesive-free assembly are disclosed. In some embodiments, the presently disclosed modular active surface devices and methods provide adhesive-free assembly processes, such as, but not limited to, laser beam welding (LBW) processes, ultrasonic welding processes, heat welding processes, chemical bonding processes, mechanical compression processes, and the like. In some embodiments, the modular active surface devices and methods provide a reagent hopper or well that is out-of-plane with the reaction chamber.

A method of manufacturing a microfluidic system or microfluidic device having at least one channel includes providing a base sheet, providing a deformable intermediate layer, providing a cover film, and laminating the base sheet, the intermediate layer and the cover film so that a back surface of the intermediate layer is attached to a front surface of the base sheet and a back surface of the cover film is attached to a front surface of the intermediate layer opposite to the back surface thereof, thereby forming a laminate comprising the base sheet, the intermediate layer and the cover film. Further, the method includes applying pressure to the front surface of the intermediate layer through the cover film so as to deform the intermediate layer, thereby forming the at least one channel. The invention also relates to a microfluidic system or microfluidic device) manufactured by this method.

Function fabrication in a microfluidic device manufactured with a custom 3D printer. The functions may include, for example, transporting or routing fluid, fluid mixing through flow and/or diffusion, blocking fluid (valve), pumping fluid, providing chemical reaction regions, providing analyte capture regions, and providing analyte separation regions. The fluid may be a liquid or a gas.

Provided is a microfluidic device capable of removing microbubbles in a channel by using a porous thin film, the microfluidic device comprising: an upper panel comprising a microfluidic channel through which a fluid passes; a porous thin film attached to the bottom surface of the microfluidic channel so as to remove microbubbles included in the fluid that passes through the microfluidic channel; a lower panel contacting the bottom surface of the porous thin film and the upper panel, a path being provided in the lower panel so as to discharge microbubbles, which pass through the porous thin film, to the outside; and a vacuum-suctioning means for vacuum-suctioning the upper panel and the lower panel such that the microfluidic channel, to which the porous thin film is attached, is attached to the lower panel in a vacuum state.

A method for manufacturing a microfluidic device can include providing a base component to define a first portion of the microfluidic device. A cap component of the microfluidic device can be fabricated with a sealing lip extending a first distance from a first side of the cap component and a support portion extending a second distance, less than the first distance, from the first side of the cap component. The method can include positioning the cap component and the base component within a mold to bring the sealing lip of the cap component in contact with the base component. The base component, the support portion of the cap component, and the sealing lip of the cap component together can define a cavity. The method can include injecting a polymer material into the mold to cause the polymer material to fill the cavity.

A method of producing a microfluidic device, including providing at least two solid layers and at least one reagent disc comprising a support disc carrying at least one dry reagent, arranging the reagent disk(s) and stacking the solid layers to form a microfluidic channel arrangement including at least one opening into a channel of the microfluidic channel arrangement and wherein the reagent disk(s) is located in the microfluidic channel arrangement.

A flow cell having at least one storage zone connected to a duct for conducting fluid out of, into or/and through the storage zone. The duct includes a duct section which is delimited by a substrate and a film joined to the substrate and in which the duct is sealed and can be opened at a predetermined breaking point by deflecting the film. The film covers a recess in the substrate which forms the duct section. A sealing wall that seals the duct and is integrally joined to the substrate is placed in the recess. The predetermined breaking point is formed by a breakable joining region between the film and an edge portion of the sealing wall facing the film. The dimensions of a peripheral area of the sealing wall is formed in the edge portion and runs parallel to the film determine the surface area of the joining region.

This present disclosure provides devices, systems, and methods for performing point-of-care analysis of a target analyte in a biological fluid via a binding assay. The present disclosure includes a cartridge for collecting the target analyte contained in a fluid sample and performing an assay. The cartridge includes an assay stack having a first separation layer, a second separation layer, and a detection membrane. The cartridge also includes a plurality of first complexes comprising a capture molecule and a magnetic bead and a plurality of second complexes comprising a detection molecule and a detection label. Further, the detection membrane includes a substrate that interacts with the detection label to elicit a quantifiable response in the presence of the target analyte. The quantifiable response corresponds to an amount of detection antibody present in the detection membrane, and the amount of detection antibody present corresponds to an amount of the target analyte present.

A device enables a user to detect biomarkers, and includes an element that defines a multiplicity of microfluidic channels that communicate between an inlet duct and an outlet duct, the inlet duct communicating with an inlet port into which a user can introduce a drop of body fluid; the outlet duct communicating with an outlet port. A resilient bladder is connected to the outlet port to provide suction. Each microfluidic channel defines a reaction chamber containing a biomarker-sensitive reagent which provides a color or a change of color in the presence of a biomarker, there being a multiplicity of different biomarker-sensitive reagents, one such biomarker-sensitive reagent being provided in each of the multiplicity of different microfluidic channels. At least part of the element is transparent so the color within the reaction chamber can be seen. The device includes a cover with magnifying lenses above the reaction chambers. The device may be used in conjunction with a smart phone.

A fluid device composite member includes: a silicone member that includes a body part which is made of silicone and which has a flow-path-defining section for defining a flow path on one surface of the body part, and that includes barrier layer having hydrophilicity or hydrophobicity disposed in at least a portion of the flow-path-defining section; and a resin substrate disposed on another surface of the body part opposite to the one surface. This method for manufacturing the fluid device composite member includes a layered body manufacturing step in which a liquid silicone material is placed on a surface of the resin substrate, and the liquid silicone material is cured at a temperature of 100° C. or less to obtain a layered body in which a silicone cured product is bonded to the resin substrate.