A fluidic chip comprising: a sealing layer having an upper surface and a lower surface; and a formed part comprising a generally planar body having a lower surface sealed with the upper surface of the sealing layer, the generally planar body having a number of through holes and a number of wells in fluid communication with the number of through holes, wherein together with the upper surface of the sealing layer, the number of through holes and the number of wells respectively define a number of fluid inlets and a number of fluid chambers in fluid connection with each other in the fluidic chip.

A receptacle holder having a base body, a first perimeter wall which at least partially protrudes from the base body, and a plurality of resilient elements, which are distributed about a first axis of the receptacle holder and are detachably connected with the first perimeter wall, and wherein each resilient element comprises a first end portion and a second end portion, and wherein each second end portion rests hooked over an edge of a portion of the first perimeter wall, and wherein the receptacle holder is configured to allow sliding of each second end portion in a direction perpendicular to the first axis.

An object is to provide a fluid handling device which can be easily manufactured and which makes it possible for a valve to be easily opened and closed with a small force. A fluid handling device for achieving the above object includes a first region; a second region; and a valve disposed between the first region and the second region, in which the valve includes: a groove-shaped valve seat disposed in a board, and a flexible layer covering the groove-shaped valve seat, and a surface of the flexible layer facing the groove-shaped valve seat is a flat surface, a protrusion is disposed on a side of the flexible layer opposite to the groove-shaped valve seat and at a position corresponding to the groove-shaped valve seat.

The invention provides systems for handling biofluids including the transport, capture, collection, storage, sensing, and/or evaluation of biofluids released by tissue. Systems of some aspects provide a versatile platform for characterization of a broad range of physical and/or chemical biofluid attributes in real time and over clinically relevant timeframes. Systems of some aspects provide for collection and/or analysis of biofluids from conformal, watertight tissue interfaces over time intervals allowing for quantitative temporal and/or volumetric characterization of biofluid release, such as release rates and release volumes.

An integrated testing device and fluid module are disclosed, as well as a method of manufacture. Fluid module contains a reservoir containing a test fluid, and a control vessel. The reservoir discharges test fluid into the control vessel, which discharges the test fluid in a controlled way to a test component.

Provided herein is a lateral flow diagnostic device and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The first end may further comprise a first region loaded with a detectable ligand, a CRISPR effector system, a detection construct, a first test band comprising a biotin ligand, and a second test band comprising a capture molecule for the detectable ligand. The detection construct may comprise an RNA oligonucleotide, having a first molecule such as FITC on a first end and a second molecule such as FAM on a second end. Contacting the sample loading portion with a sample causes the sample to flow from the sample loading portion of the substrate towards the first and second capture regions, thereby generating a detectable signal, which may be indicative of a disease state.

The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.

There is provided a sample processing cartridge comprising a. a sample entry location; b. a closed sample processing chamber; c. a sample analysis location comprising a sample analysis well; d. a first channel fluidly connecting the sample entry location and the sample processing chamber; e. a second channel connecting the sample analysis location and the sample processing chamber, the second channel comprising a closed or closable second channel valve;
wherein the sample processing chamber comprises a second channel port providing fluid connection between the second channel and the sample processing chamber, the second channel port being positioned in a sample accumulating region of the sample processing chamber.

There is also provided a sample processing system comprising the cartridge, and methods of use of the cartridge and processing system in a sample processing assay.

Microfluidic systems, pumps, valves and applications of the same are provided. The microfluidic system may be a pump or a valve having a fluidic chip and an actuator controlling the opening and closing of the fluidic channel in the fluidic chip. The actuator may be disposed to tilt from the fluidic chip, forming a tilted-rotor peristaltic pump. Alternatively, the actuator may be a rolling ball actuator, and different fluidic chips may be used in different applications. For example, the fluidic chip may be a spiral pump chip having spiral channels, a rotary peristaltic pump chip having multiple output channels, or a multi-port valve chip having one port interconnected with multiple different ports. An analytical valve chip may switchably interconnect bioreactor and rinse/calibration input channels to sensor and waste output channels. The actuator of a random-access valve can move from one valve position to another without opening or closing intermediate ones.

A microfluidic mixer, formed by two parts, a first part being a substrate having formations defining fluid channels on an outer surface that is directed towards a second part, which is a flexible layer. The flexile layer has formations defining a fluid channel which, when the flexible layer is positioned over the substrate so as to cover the fluid channels of the substrate provides a fluid communication path. A section of said communication path comprises at least first and second fluid channels for providing first and second fluids. The first and second fluid channels merge before an inlet of a mixing chamber. The mixing chamber comprises perturbation formations. An outlet of the mixing chamber is connected to an outlet fluid channel. The flexible layer comprises points for compression at the inlet and outlet of the mixing chamber for closing the merged fluid channel. The perturbation formations of the mixing chamber are vertically arranged vertically with respect to an inner surface.