Provided are a multi-channel isothermal amplification system and an operation method. The operation method comprises: a first step in which sample tubes are disposed in holes formed in a line in a heating block, respectively, wherein the heating block comprises: a first heating block area in which some of the plurality of holes are formed in a line; and a second heating block area in which the rest of the plurality of holes are formed in a line, and which is disposed to be in a line with the first heating block area, wherein the first heating block area and the second heating block area are spaced apart from each other at an interval as much as one hole area between two holes; and a second step in which an optical system moves in a longitudinal direction of the first heating block area and the second heating block area.

The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.

The present disclosure relates to method and systems for amplifying nucleic acid molecule and quantify amplification thereof, with a polymerase chain reaction (PCR) or a loop-mediated isothermal amplification (LAMP), through bulk heating a biological enzymatic reaction mixture in solution containing nucleic acid templates, polymerase enzyme, and chemically modified nanoparticles. The method and system may comprise quantify amplification by irradiating the biological enzymatic reaction mixture during an annealing and/or elongation steps with an ultraviolet (UV) light source.

This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

Disclosed are methods and apparatus for solar-thermal microfluidic polymerase chain reaction. A device comprises a microfluidic chip including at least one PCR region, an energy absorption layer disposed adjacent to the microfluidic chip, a solar energy concentrator adapted to produce a plurality of temperature profiles on the microfluidic chip adapted to facilitate PCR, and a photomask disposed between the solar energy concentrator and the microfluidic chip.

The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.

A gene amplification chip may include a substrate; a through-hole array including through-holes that extend from an upper surface of the substrate to a lower surface of the substrate and in which a gene amplification reaction occurs; and a photothermal film provided on at least one of the upper surface and the lower surface of the substrate and configured to generate heat using light.

Systems and methods for plasmonic heating by combined use of thin plasmonic film-based 2D and 3D structures and a light-emitting diode (LED) for nucleic acids amplification through fast thermal cycling of polymerase chain reaction (PCR) are described.

A method of collecting resin beads includes first to fourth steps. The first step is a step of preparing a sample on a thin film provided on an upper surface of a substrate. The second step is a step of irradiating the thin film with a laser beam and a laser beam with the laser beam and the laser beam being distant from each other. The third step is a step of producing a microbubble at a position irradiated with the laser beam and producing a microbubble at a position irradiated with the laser beam, by heating the sample by irradiation with the laser beams. The fourth step is a step of collecting a plurality of resin beads in a region between the microbubble and the microbubble by producing convection of the sample in a direction perpendicular to a direction of alignment of the microbubble and the microbubble.