Processes and compositions are provided for performing magnetibuoyant separations of different biomolecules (e.g., cells, organelles, etc.) in a biological sample, as well as compositions and kits for performing such methods. Compositions containing the separated biomolecules, and methods for using the same for in-vitro and in-vivo biomedical applications, are also provided. The magnetibuoyant methods of the invention employ targeted magnetic particles, preferably targeted nanomagnetic particles, and targeted buoyant particles such as buoyant microparticles and microbubbles. Among the benefits of the invention is the ability to combine targeted magnetic particles with differentially targeted buoyant particles to achieve separation of two or more specifically cell targeted populations during the same work flow.

Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

The present invention discloses a tumor molecular detection/diagnostic reagent, which takes excrement as a detection sample and includes an SDC2 gene methylation detection reagent. The methylation level of the SDC2 gene detected in the excrement has an extremely high relevance to the onset of the colorectal cancer. The sensitivity of the SDC2 gene in the excrement is 87 percent and the specificity is up to 98 percent or even higher than that in tissue.

The present invention discloses a tumor molecular detection/diagnostic reagent, which takes excrement as a detection sample and includes an SDC2 gene methylation detection reagent. The methylation level of the SDC2 gene detected in the excrement has an extremely high relevance to the onset of the colorectal cancer. The sensitivity of the SDC2 gene in the excrement is 87 percent and the specificity is up to 98 percent or even higher than that in tissue.

The invention discloses a multi-stage suspension magnetizing roasting-magnetic separation system and method for refractory iron ore, which belongs to the field of mineral processing technology. This system comprises multistage suspension preheater, multistage suspension oxidizer, multistage suspension redactor, on-line grade analyzer, ore-like splitter, magnetic separator, dust remover, roots blower and other components and connection modes. The refractory iron ore treated by the present method can be produced to homogeneous magnetite or maghemite accurately, and through magnetic separation, on-line grade analyzer detection and ore-like splitter, the concentrate powder which reach the set grade can be obtained, and the unqualified ore powder enters the next stage of oxidation-reduction-magnetic separation-split treatment. Through the present system and method, products with different roasting quality can be obtained, and can avoid the phenomenon of over burning or under burning occurring in the past process and equipment.

The invention discloses a multi-stage suspension magnetizing roasting-magnetic separation system and method for refractory iron ore, which belongs to the field of mineral processing technology. This system comprises multistage suspension preheater, multistage suspension oxidizer, multistage suspension redactor, on-line grade analyzer, ore-like splitter, magnetic separator, dust remover, roots blower and other components and connection modes. The refractory iron ore treated by the present method can be produced to homogeneous magnetite or maghemite accurately, and through magnetic separation, on-line grade analyzer detection and ore-like splitter, the concentrate powder which reach the set grade can be obtained, and the unqualified ore powder enters the next stage of oxidation-reduction-magnetic separation-split treatment. Through the present system and method, products with different roasting quality can be obtained, and can avoid the phenomenon of over burning or under burning occurring in the past process and equipment.

A method of depleting a given analyte from a water source is provided. The method is applicable to water used in oil recovery, water used in natural gas recovery, the treatment of water wells, and for water used in hydraulic fluids for fracturing processes, such as water to be used in proppants or fracking fluids. The method involves depleting an analyte from a water source, said method comprising contacting a water source with a superparamagnetic or paramagnetic nanoparticle; complexing the analyte with the particle; and removing the analyte-particle complex by applying a magnetic field so as to provide a water source with depleted analyte content. The depleted water can then be pumped into one or more connecting injection well(s) in an oil field pushing the crude oil 10 towards one or more production well(s) thereby allowing for enhanced oil recovery from the production wells.