A modular cassette is provided for separating a composite fluid into at least two component parts thereof during centrifugation. The modular cassette includes: a housing defining a fluid inlet, a fluid outlet, and a chamber for fluid separation; a fluidic channel configured to provide fluid communication between at least two components of the modular cassette; a heat expanding valve including: a flow pathway including undulations configured to facilitate closing of the fluidic channel, wherein the heat expanding valve occludes one or more of the undulations of the flow pathway to close the fluidic channel; and a heating element configured to actuate the heat expanding valve.

A fluid separation device includes a centrifuge in which a fluid is separated into at least two components, with an interface therebetween. At least a portion of one of the separated fluid components is removed from the centrifuge and flows through a vessel. Light is reflected off of the separated fluid component in the vessel and received and analyzed to determine its main wavelength. If the main wavelength is higher than a maximum value, a target location of the interface is changed. If the main wavelength is less than the maximum value, then the location of the interface is compared to the target location. When the interface is sufficiently close to the target location, the optical density of the separated fluid component in the vessel is compared to a minimum value. If the optical density is less than the minimum value, the target location of the interface is changed.

Embodiments relate to systems, chambers, and methods for processing particles by washing, concentrating, and/or treating the particles. Some embodiments provide for the processing of cells that may be in a liquid medium (e.g., a liquid in which the cells were grown) and are processed by being washed and concentrated for later use in research or other therapies.

This invention relates to a continuous flow centrifugation device and method of using the device for separation of solids, particles, liquids, or gases from a mixture containing them. The device contains rotating separation chamber in which incoming material in continuously enters the chamber via an inlet and the separated materials continuously flow out via two outlets. Solids or heavier materials exit from an outlet tubing, whose opening is placed in a space that is farthest from the center of rotation and has the highest centrifugal force. The liquid/supernatant outlet is placed in the space that is closer to the center of rotation with the lowest centrifugal force. The reference of placement of inlet to solids outlet is in the same direction as the direction of rotation so that the flow of incoming material, directed from inlet to solids outlet, is in the same direction as the direction of rotation. All product contact surfaces can be disposable and diverse separation methods for different applications can be executed in a fully automated or manual manner by a controller that manages different inputs and outputs of system components.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

Embodiments relate to systems, chambers, and methods for processing particles by washing, concentrating, and/or treating the particles. Some embodiments provide for the processing of cells that may be in a liquid medium (e.g., a liquid in which the cells were grown) and are processed by being washed and concentrated for later use in research or other therapies.

Embodiments are described for separating collecting components from a multi-component fluid such as whole blood. Some embodiments provide for controlling the amount of a component, such as platelets, introduced into a separation chamber to ensure that the density of fluid in the separation chamber does not exceed a particular value. This may provide for collecting purer components. Other embodiments may provide for determining a chamber flow rate based on a concentration of a component in the multi-component fluid, which may then be used to determine a centrifuge speed, to collect purer concentrated components.

A centrifugal field-flow fractionation device includes an annular rotor, an arc-shaped channel member, a rotation drive unit, and a restriction unit. A channel member 16 is provided along an inner peripheral surface of the rotor, has therein a channel 161 for a liquid sample by laminating a plurality of layers, and has an inlet for the liquid sample to the channel 161 and an outlet for the liquid sample from the channel 161. By rotating the rotor, particles in the liquid sample in the channel 161 are classified by centrifugal force. A restriction spacer 64 restricts the channel 161 from being compressed to a height less than a certain height when the channel member 16 is compressed and deformed in a laminating direction.

The present disclosure relates to methods and devices for the separation of blood components including separation by rapid sedimentation, including in an automated fashion.