Described in several example embodiments herein are NUDIX overexpressing plants and uses thereof. In some embodiments, the NUDIX overexpressing plants are used for phytoremediation.

A fungus having manganese oxidation capacity is provided. The fungus can oxidize Mn.sup.2+ in a water body into a water-insoluble manganese oxide; and the Mn.sup.2+ oxidizing fungus is Cladosporium sp. XM01 strain with the accession number of CGMCC NO. 21083. The Cladosporium sp. XM01 strain is used to oxidize Mn.sup.2+ in a natural water body, and has stable operation within a range of room temperature (15-30 C.) and a range of neutral pH (6.0-7.5) and high Mn.sup.2+ oxidation efficiency; moreover, the XM01 strain may oxidize Mn.sup.2+ cyclically, thereby achieving the in-situ remediation of water bodies or soils polluted by heavy metals or trace organic substances. The manganese oxides generated through oxidization in the growth process of the strain have a good application potential in sewage treatment, water environment restoration, soils and other fields.

An in-situ mycoremediation system and process is provided, including a device with a rod casing having a top end, a bottom end, and a sidewall with one or more perforations, the sidewall defining an internal channel that extends from an intake opening on the top end to the one or more perforations, a sleeve that extends around at least part of the rod casing and that is slidable between at least a first position that covers the one or more perforations and a second position that at least partly uncovers the one or more perforations, and a plumbing line linked to the intake opening and configured to facilitate forcible injection of one more fungal mixtures and/or air via the one or more perforations when the sleeve is in the second position.

Some embodiments of the disclosure are directed to systems and methods to treat citrus greening. In some embodiments, one or more samples are taken from a location where citrus greening occurs or may occur. In some embodiments, native microorganisms are isolated and propagated. In some embodiments, the microorganisms include algae. In some embodiments, propagated native algae is returned to an area affected by citrus greening. In some embodiments, the returned propagated native algae is distributed to a concentration of 10 times or more of the original native algae concentration. In some embodiments, the distributed algae increase natural antibiotics and nutrients in the soil resulting in prevention of citrus greening.

The present disclosure provides a fungus and a microbial agent for treating mercury contamination, use thereof, a mercury removal method, and a method for identifying a fungus capable of treating mercury contamination, and relates to the technical field of biological mercury removal. The present disclosure provides a Metarhizium fungus and 8 species of non-Metarhizium fungi for treating mercury contamination, a method for identifying a fungus capable of treating mercury contamination, and enzymes for removing methylmercury and divalent mercury, which provides a genetic basis for treating mercury contamination with recombinant fungi or bacteria.

An in-situ mycoremediation system and process is provided, including a device with a rod casing having a top end, a bottom end, and a sidewall with one or more perforations, the sidewall defining an internal channel that extends from an intake opening on the top end to the one or more perforations, a sleeve that extends around at least part of the rod casing and that is slidable between at least a first position that covers the one or more perforations and a second position that at least partly uncovers the one or more perforations, and a plumbing line linked to the intake opening and configured to facilitate forcible injection of one more fungal mixtures and/or air via the one or more perforations when the sleeve is in the second position.

An in-situ mycoremediation system and process is provided, including a device with a rod casing having a top end, a bottom end, and a sidewall with one or more perforations, the sidewall defining an internal channel that extends from an intake opening on the top end to the one or more perforations, a sleeve that extends around at least part of the rod casing and that is slidable between at least a first position that covers the one or more perforations and a second position that at least partly uncovers the one or more perforations, and a plumbing line linked to the intake opening and configured to facilitate forcible injection of one more fungal mixtures and/or air via the one or more perforations when the sleeve is in the second position.

An Aspergillus fumigatus for degrading soil microplastics and an application thereof are provided. The Aspergillus fumigatus has a preservation name of Aspergillus fumigatus F and is deposited at the China General Microbiological Culture Collection Center on Sep. 18, 2024 under CGMCC NO. 41513. The Aspergillus fumigatus F may grow in an environment with PE and PET as the only carbon sources, and may be used as a biodegradable microbial inoculum applied to harmless treatment, recycling and environmental restoration of microplastic resources.

An in-situ mycoremediation system and process is provided, including a device with a rod casing having a top end, a bottom end, and a sidewall with one or more perforations, the sidewall defining an internal channel that extends from an intake opening on the top end to the one or more perforations, a sleeve that extends around at least part of the rod casing and that is slidable between at least a first position that covers the one or more perforations and a second position that at least partly uncovers the one or more perforations, and a plumbing line linked to the intake opening and configured to facilitate forcible injection of one more fungal mixtures and/or air via the one or more perforations when the sleeve is in the second position.

The present invention is directed to a phytoremediation system and related methods of forming and using such phytoremediation system. A phytoremediation system may include one or more plants, such as one or more trees, and one or more porous materials. The one or more porous materials may include a glass-based porous material. The one or more porous materials may include a biomolecular porous material.