A method for removing pollutants by using a mixed microbial solution of beneficial microorganisms to enhance electrokinetic force. It includes a mixed microbial solution; an anode tank and a cathode tank are connected to an external power supply. A soil tank contains contaminant soil and the soil tank is positioned between the anode tank and the cathode tank which are parallel to each other. A soil-retaining net is disposed over the soil. The external direct current is used to allow the mixed microbial solution to transfer in the soil tank.

A growing unit for biological hydrosynthesis, energy generation and storage and/or topsoil restoration, the growing unit comprising: a container configured for growing plants and containing a growth media located therein; a reservoir located in a lower portion of the container and associated with an outlet portion of the container, and a substantially vertical liquid inlet pipe associated with the reservoir, wherein the growth media comprises a mixture including a first catalyst, wherein the first catalyst stimulates formation of a humified soil and wherein the growth media is amended with an irrigation liquid which stimulates biological activity in the growth media and in and adjacent to the reservoir.

A method of treating a heavy metal contaminated soil including contacting the contaminated soil with a bioremediation mixture for a predetermined time such that the contaminated soil is anaerobically digested, wherein the contaminated soil contains one or more heavy metals, and wherein the anaerobically digested soil contains a lesser amount of the one or more heavy metals than the contaminated soil.

This invention relates to a method of in-situ remediation of arsenic-contaminated soil, comprising the following steps: inoculating a bacterial strain, Pseudomonas putida MnB1 in a culture medium where an addition amount of the bacterial strain accounts for 2-10% (v/v) of the culture medium; shaking the culture medium with the bacterial strain at a rotation speed of 100-180 rpm at 15-35° C. for 1-5 days under an aerobic condition, thereby yielding an enriched bacterial strain; and adding manganese carbonate, ammonium ferrous sulfate, sodium citrate, a yeast extract and the enriched bacterial strain to arsenic contaminated soil; adding water to the soil until the soil has a moisture content of 50-70%, stirring the soil for 5-30 minutes, and culturing the bacterial strain in the soil under an aerobic/microaerobic condition at 10-40° C. for 2-6 weeks.

Some embodiments of the disclosure are directed to systems and methods to treat citrus greening. In some embodiments, one or more samples are taken from a location where citrus greening occurs or may occur. In some embodiments, native microorganisms are isolated and propagated. In some embodiments, the microorganisms include algae. In some embodiments, propagated native algae is returned to an area affected by citrus greening. In some embodiments, the returned propagated native algae is distributed to a concentration of 10 times or more of the original native algae concentration. In some embodiments, the distributed algae increase natural antibiotics and nutrients in the soil resulting in prevention of citrus greening.

Disclosed is a method for preparing a solid material including manganese, the method including the following steps: a. bringing into contact an aqueous effluent including manganese, for example at least 5 mg/L, typically at least 5 to 50 mg/L, and preferably 7 to 25 mg/L of manganese, with an oxidizing agent, manganese, preferably at a temperature between 10° C. and 50° C., and obtaining an oxidized aqueous solution; b. adding a base to the oxidized aqueous solution obtained at the end of step a) until a pH of between 8 and 12, preferably greater than 9, and preferably from 9 to 10.5, and obtaining a solution including a precipitate; c. filtration of the solution obtained at the end of step b); and d. obtaining a solid material including manganese, and especially manganese (IV) and/or Mn (III).

A method for treating chemicals below ground is provided. The system includes contacting the chemicals with a rhizosphere to maximize transpiration rate and minimize over-saturation of the rhizosphere. Also provided is a method for using contaminated groundwater as a sole source of irrigation. The method uses extracting the contaminated water and pumping the extracted water to an irrigation zone under the control of an autonomous irrigation system. Each zone includes some trees and the root system for each tree is modified to function as a treatment cell. The invention also provides a system for treating contaminated water, using a contaminated water source situated below ground surface. The system uses a pump for extracting contaminated water from the water source and prevents the contaminated water from directly contacting the atmosphere above the ground surface. Vegetation which defines an underground rhizosphere is adapted to directly receive the water.

Phytoremediation processes, methods, materials and compositions to remediate soil, sediment and groundwater that is contaminated by per- and polyfluoroalkyl substances (PFAS) via phytoextraction which includes the uptake and translocation of contaminants in the contaminated media by plant roots into the above ground portions of the plants. The plants can be selected from sixteen plants as well as other plants and the invention can include managing soil salinity levels of the plants, manipulating amounts of organic matter in the contaminated site media, managing pH levels of the contaminated sites, utilizing double cropping systems, utilizing double-canopy system, and managing harvest methodology of the plants. Phytoremediation can be enhanced by management of salinity levels using either CaSO4, MgSO4, or a combination of CaSO4 and MgSO4 to be within the range of approximately 360 μS/cm to approximately 5,500 μS/cm.

A decontamination unit (1) for decontaminating soil of an area (19) of the ground (16) from harmful substances, wherein the decontamination unit (1) comprises: a body (2) comprising an outer coating (3) of a fluid permeable material and an absorbent material (4), for absorbing of the harmful substances, enclosed within the outer coating (3) and a hose (5) having an intermediate perforated part (6) arranged in the absorbent material (4) inside the body (2), and a first end part (7) and a second end part (8) protruding from a respective first end (9) and second end (10) of the body (2), wherein the first end part (9) and the second end part (10) are connectable to another decontamination unit or to a fluid handling device to permit fluid communication to and from the hose (5). A method for decontaminating soil of an area (19) of the ground (16) is also described herein.

The present disclosure provides a woody plant seeding formulation for phytoremediation, ecological restoration, land reclamation, environmental rehabilitation, revitalization, revegetation, and reforestation of disturbed and degraded lands. The seeding formulation includes a mix of woody trees or shrubs seeds, organic mulch, beneficial microbial populations, mineral fertilizer, adhesive, absorbent polymer, and water. The present disclosure also provides for pre-treatment of surfaces by spreading or mixing with organic biomass with the surface. Methods of preparing and applying the seeding formulation and pre-treatment are also disclosed.