The Invention discloses an ecological remediation method for controlling sulphur pollution in black and odorous sludge of rivers. Firstly, the sludge from pollution layer of the rivers will be dredged and stacked on the banksides with slope protection built along the outer edge of the sludge storage site; then innocent pretreatment will be conducted for the sludge. Specific steps comprise solarization and ploughing, and mixed ameliorant of certain proportion will be added for modification between first solarization and intermittent ploughing. Then, large emergent aquatic plants will be planted on the stacked sludge after pretreatment. The technical method provided by the Invention may control the release of acid-volatile sulfide in the contaminated sludge on one hand and reduce concentration of volatile organic sulfide in the waters on the other hand. With simple technical process and strong operable technology, the Invention meets the current requirements for controlling sulphur pollution in black and odorous sludge of rivers in China and facilitates realizing the goal of long-term control of sulphur pollution in the deposit of the waters.

A process for constructing a soil-based rhizosphere flow-through system to break down contaminants in contaminated water. The process includes the steps of: providing plants planted in soil in a test bioreactor, the plants providing a rhizosphere; exposing the rhizosphere to the contaminated water, extracting microorganisms from the rhizosphere following their exposure to the contaminated water: preparing a microbial suspension from the extract; subjecting the microbial suspension to growth conditions to increase the concentration of the microorganism, thereby preparing a soil conditioner; adding the soil conditioner to soil in a contained area having a water flow inlet and outlet; and planting a plurality of plants in the soil, the plants being of the same species as the plants of the test bioreactor.

Methods are provided for cultivating fungi for scaled-up production of microbe-based products. Specifically, cultures of fungi, such as, e.g., Pisolithus tinctorius, are grown by aerobic submerged fermentation in liquid medium containing a particulate anchoring carrier to increase mycelial biomass.

A stacked circulatable microbial electrochemical reactor and a degradation method of petroleum hydrocarbon contaminated soil are provided, which belong to the field of microbial electrochemical soil remediation. The stacked circulatable microbial electrochemical reactor of the present disclosure expands influence range of anodes by a stacked microbial electrochemical system, accelerates the movement of the petroleum hydrocarbon molecules in the contaminated soil by a water circulation system, and improves the mass transfer capacity of soil, thereby increasing the degradation efficiency of petroleum hydrocarbon in the contaminated soil with microbial electrochemical technology from different aspects. The degradation method of petroleum hydrocarbon contaminated soil is provided. The degradation method of the present disclosure is simple in operation and has a high degradation efficiency of petroleum hydrocarbon in contaminated soil.

Disclosed is a restoration material, a restoration method for abandoned ion-absorbed rare earth tailings area and use thereof, which belongs to the technical field of ecological restoration. The restore material for the abandoned ion-absorbed rare earth tailings area provided by the present disclosure comprises AM fungi and pioneer plants; the species of AM fungi is selected from one or more of G. intraradices, G. mosseae and P. occultum; the pioneer plants is selected from one or more of paspalum, ramie and awn.

A process for constructing a soil-based rhizosphere flow-through system to break down contaminants in contaminated water. The process includes the steps of: providing plants planted in soil in a test bioreactor, the plants providing a rhizosphere; exposing the rhizosphere to the contaminated water; extracting microorganisms from the rhizosphere following their exposure to the contaminated water; preparing a microbial suspension from the extract; subjecting the microbial suspension to growth conditions to increase the concentration of the microorganisms, thereby preparing a soil conditioner; adding the soil conditioner to soil in a contained area having a water flow inlet and outlet; and planting a plurality of plants in the soil, the plants being of the same species as the plants of the test bioreactor.

A nanobioremediation method is for soil in a high-concentration polycyclic aromatic hydrocarbon (PAH)-contaminated site. The method includes adding a carbon nanomaterial and a base fertilizer to soil in a high-concentration PAH-contaminated site, evenly broadcast sowing alfalfa seeds to the contaminated soil, and keeping the soil moisture content at 60% of the field moisture capacity, and when the alfalfa grows to the flowering or maturity stage, harvesting and removing the plants from the contaminated soil.

The present invention discloses a strain of Lysinibacillus fusiformis with methylamine degradability and the application thereof. This strain, named Lysinibacillus fusiformis GDUTAN2, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University, Wuhan City, Hubei Province with a deposit number of CCTCC NO. M 2017284. This Lysinibacillus fusiformis GDUTAN2 was Grain-positive and rod-shaped, and the colony appeared to be round, white and transparent, having a diameter of 1-2 mm. The Lysinibacillus fusiformis GDUTAN2 of the present invention can be applied to environmental restoration, degrading methylamine in the environment at a high degradation efficiency. When it degraded methylamine for 96 h at a substrate concentration of 130 mg/L, the degradation efficiency could reach 32.8%.

Phytoremediation processes, methods, materials and compositions to remediate soil, sediment and groundwater that is contaminated by per- and polyfluoroalkyl substances (PFAS) via phytoextraction which includes the uptake and translocation of contaminants in the contaminated media by plant roots into the above ground portions of the plants. The plants can be selected from sixteen plants as well as other plants and the invention can include managing soil salinity levels of the plants, manipulating amounts of organic matter in the contaminated site media, managing pH levels of the contaminated sites, utilizing double cropping systems, utilizing double-canopy system, and managing harvest methodology of the plants. Phytoremediation can be enhanced by management of salinity levels using either CaSO4, MgSO4, or a combination of CaSO4 and MgSO4 to be within the range of approximately 360 μS/cm to approximately 5,500 μS/cm.

The phytoremediation process for removing hazardous metals, such as Be, Cr, Co, Mn, and Ra, from contaminated soil and/or coal fly ash, e.g. in a landfill, includes growing plants in the soil and/or fly ash; incorporating a root growth inducing auxin in the soil and/or fly ash in the vicinity of the growing plants and subsequently cultivating the plants until a predetermined increase in root mass of the plant roots has occurred. The preferred auxin is 3-4-deoxy-glucosamine, which was found to increase plant root mass by 200% to 800%. An organic synthesis using glucosamine as starting material or a bioengineering process starting with commercial micronized shrimp meal and/or crab meal moistened with water can provide the 3-4-deoxy-glucosamine. Economical embodiments of the process use plants that produce marketable products and recovers valuable metals.