The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom.

Described herein is a process for producing saccharides and ethanol from biomass feedstock that includes (a) producing an enzyme composition by culturing a fungal strain(s) in the presence of a lignocellulosic medium, (b) using the enzyme composition to saccharify the biomass feedstock, and (c) fermenting the saccharified biomass feedstock to produce ethanol. The process is scalable and, in certain aspects, is capable of being deployed on farms, thereby allowing local production of saccharides and ethanol and resulting in a reduction of energy and other costs for farm operators. Optional steps to improve the biomass-to-fuel conversion efficiency are also contemplated, as are uses for byproducts of the process described herein.

Disclosed herein are compositions and products of Arundo grass plants and methods of producing the same.

A coal refuse remediation process is described. The process includes providing coal refuse and blending a slag with at least a portion of the coal refuse to form a medium. The coal refuse has a particle size. The medium has a pH within a range of about 3.5 and about 10 and the medium facilitates growth of vegetation when applied to a coal refuse pile at a thickness of less than about 24 inches.

Described herein is a process for producing saccharides and ethanol from biomass feedstock that includes (a) producing an enzyme composition by culturing a fungal strain(s) in the presence of a lignocellulosic medium, (b) using the enzyme composition to saccharify the biomass feedstock, and (c) fermenting the saccharified biomass feedstock to produce ethanol. The process is scalable and, in certain aspects, is capable of being deployed on farms, thereby allowing local production of saccharides and ethanol and resulting in a reduction of energy and other costs for farm operators. Optional steps to improve the biomass-to-fuel conversion efficiency are also contemplated, as are uses for byproducts of the process described herein.

A method, for mitigating an environmental condition, may include assessing a geographic area with which the environmental condition is associated; and creating a plan to mitigate the environmental condition. The plan may identify a bio-crop for mitigating the environmental condition and a bio-product to be produced from the bio-crop. The method may also include planting the bio-crop in soil that is located within the geographical area. The bio-crop may be planted in a manner that enables the environmental condition to be mitigated. The method may further include harvesting the bio-crop based on planting the bio-crop; processing the harvested bio-crop to obtain biomass; producing the bio-product based on the biomass; and outputting the bio-product based on producing the bio-product.

A method for treating soil in situ or ex situ to reduce the concentration of contaminating or polluting organic chemical compounds is provided. The method comprises steps of: a) determining a contaminated/polluted area where soil is to be treated; b) adding a fertilizer in the form of warm-composted manure or other compost having a nutrient content corresponding to warm composted manure and in which the nutrients are correspondingly hard bound to the compost to a soil layer in the area, the fertilizer being added in an amount of 10% (weight percentage) or more of soil of the soil layer; c) adding iron, unless the soil of the soil layer already comprises at least 2 kg of iron per ton of soil; d) adjusting soil pH to an acidic pH; and e) keeping the soil of the soil layer moist by adding water as needed for a predetermined period.

The present invention is directed toward a bioremediation product, its method of manufacture and method of use. A blend of microorganisms, cleansers and surfactants is disclosed which is non-toxic and solvent free, and which has the ability to accelerate the breaking down process of oils, materials such as benzene, toluene, and xylene (BTEX); petroleum hydrocarbon fractions F1-F4 (as defined by CCME2000), polycyclic aromatic hydrocarbons (PAH), paraffin waxes, volatile organics, molds, and the like; while simultaneously providing a biological component which is effective to reduce the total petroleum hydrocarbon levels to zero at an accelerated rate. The product is useful for bioremediation and as an oil dispersant composition useful in treating oil spills, and for cleanup of the shoreline, animals, plants, and equipment.

Biocatalytic composition to be used in the agricultural, zootechnical and environmental recovery fields, to transform substrates which is reacted with, to non-polluting substances, including a component with coenzymatic activity including Vitamin A, Vitamin D3, Vitamin E, Propylgallate, Raw fats, Raw proteins; a component with enzymatic activity including Bacillus licheniformis, Bacillus subtilis, Bacillus thuringiensis, Bacillus SPP, Aspergillus Oryzae, Aspergillus Niger, Lactobacillus bifidus, Lactobacillus acidophilus, Amylase, Protease, Lipase, Cellulase, Gumase; and a component including substances regulating pH, including Humic acids, Fulvic acids, Crenic acids, Apocrenic acids, Vitamin A, Vitamin D3, Vitamin PP, Arthrospira maxima, among others.

The present invention provides a method of remediating contaminated soil with heavy metal using a microorganism of Sporosarcina sp. Contaminated soil is inoculated with the Sporosarcina sp. KM-01 (Sporosarcina pasteurii KM-01) strain includes a base sequence of SEQ ID NO. 1, the Sporosarcina sp. KM-07 (Sporosarcina pasteurii KM-07) strain includes a base sequence of SEQ ID NO. 2, and the Sporosarcina sp. KM-12 (Sporosarcina pasteurii KM-12) strain includes a base sequence of SEQ ID NO. 3. The strains are capable of producing urease.